ID   RAW-Difluo mLC3
AC   CVCL_A8CB
DR   InvivoGen; rawdf-mlc3
DR   Wikidata; Q107116715
CC   Characteristics: Allows monitoring of autophagic flux in real time by detecting the appearance of dual red and green RFP:GFP:LC3 puncta or single red RFP:LC3 puncta by fluorescence microscopy. Early in autophagy, both RFP and GFP signals are detected. As the fusion of the autophagosomes with the lysosomes progresses, the GFP fluorescence diminishes, leaving only the RFP fluorescence visible. The percentages of RFP-GFP positive and of RFP positive cells can be determined and these values can be used to assess autophagic flux (InvivoGen=rawdf-mlc3).
CC   Characteristics: Stably expresses a RFP-GFP-Map1lc3b construct (InvivoGen=rawdf-mlc3).
CC   Genetic integration: Method=Transfection; Gene=FPbase; EGE3L; DsRed (Note=drFP583, red fluorescent protein from Discosoma sp.).
CC   Genetic integration: Method=Transfection; Gene=FPbase; R9NL8; eGFP (Note=Enhanced GFP).
CC   Genetic integration: Method=Transfection; Gene=MGI; MGI:1914693; Map1lc3b.
CC   Genetic integration: Method=Transfection; Gene=UniProtKB; P17493; Streptoalloteichus hindustanus ble (BleoR).
CC   Transformant: NCBI_TaxID; 11788; Abelson mouse leukemia virus (AbMuLV).
CC   Derived from site: In situ; Ascites; UBERON=UBERON_0007795.
CC   Cell type: Macrophage; CL=CL_0000235.
CC   Breed/subspecies: BALB/c.
DI   NCIt; C21604; Mouse leukemia
OX   NCBI_TaxID=10090; ! Mus musculus (Mouse)
HI   CVCL_0493 ! RAW 264.7
SX   Male
AG   Adult
CA   Cancer cell line
DT   Created: 20-05-21; Last updated: 10-04-25; Version: 9
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