ID   RAW-Difluo mLC3
AC   CVCL_A8CB
DR   Wikidata; Q107116715
WW   https://www.invivogen.com/raw-difluo-mlc3
CC   Characteristics: Allows monitoring of autophagic flux in real time by detecting the appearance of dual red and green RFP:GFP:LC3 puncta or single red RFP:LC3 puncta by fluorescence microscopy. Early in autophagy, both RFP and GFP signals are detected. As the fusion of the autophagosomes with the lysosomes progresses, the GFP fluorescence diminishes, leaving only the RFP fluorescence visible. The percentages of RFP-GFP positive and of RFP positive cells can be determined and these values can be used to assess autophagic flux (InvivoGen).
CC   Characteristics: Stably expresses a RFP-GFP-Map1lc3b construct.
CC   Transfected with: MGI; MGI:1914693; Map1lc3b.
CC   Transfected with: UniProtKB; Q9U6Y8; Discosoma sp. red fluorescent protein drFP583 (DsRed, RFP).
CC   Transfected with: UniProtKB; P42212; GFP (with p.Phe64Leu, p.Ser65Thr and p.His231Leu = EGFP).
CC   Transfected with: UniProtKB; P17493; Streptoalloteichus hindustanus ble (BleoR).
CC   Transformant: NCBI_TaxID; 11788; Abelson mouse leukemia virus (AbMuLV).
CC   Derived from site: In situ; Ascites; UBERON=UBERON_0007795.
CC   Cell type: Macrophage; CL=CL_0000235.
CC   Breed/subspecies: BALB/c.
DI   NCIt; C21604; Mouse leukemia
OX   NCBI_TaxID=10090; ! Mus musculus (Mouse)
HI   CVCL_0493 ! RAW 264.7
SX   Male
AG   Adult
CA   Cancer cell line
DT   Created: 20-05-21; Last updated: 30-01-24; Version: 7
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