ID   THP1-Difluo hLC3
AC   CVCL_A8BZ
DR   cancercelllines; CVCL_A8BZ
DR   InvivoGen; thpdf-hlc3
DR   Wikidata; Q107117044
CC   Population: Japanese.
CC   Characteristics: Allows monitoring of autophagic flux in real time by detecting the appearance of dual red and green RFP:GFP:LC3 puncta or single red RFP:LC3 puncta by fluorescence microscopy. Early in autophagy, both RFP and GFP signals are detected. As the fusion of the autophagosomes with the lysosomes progresses, the GFP fluorescence diminishes, leaving only the RFP fluorescence visible. The percentages of RFP-GFP positive and of RFP positive cells can be determined and these values can be used to assess autophagic flux (InvivoGen=thpdf-hlc3).
CC   Characteristics: Stably expresses a RFP-GFP-MAP1LC3B construct (InvivoGen=thpdf-hlc3).
CC   Sequence variation: Gene fusion; HGNC; HGNC:2457; CSNK2A1 + HGNC; HGNC:13917; DDX39B; Name(s)=CSNK2A1-DDX39B (from parent cell line).
CC   Sequence variation: Gene fusion; HGNC; HGNC:7132; KMT2A + HGNC; HGNC:7136; MLLT3; Name(s)=KMT2A-MLLT3, MLL-MLLT3, MLL-AF9 (from parent cell line).
CC   Sequence variation: Mutation; HGNC; HGNC:7989; NRAS; Simple; p.Gly12Asp (c.35G>A); ClinVar=VCV000039648; Zygosity=Heterozygous (from parent cell line).
CC   Sequence variation: Mutation; HGNC; HGNC:11998; TP53; Simple; p.Arg174fs*3 (c.520_545del26); Zygosity=Heterozygous (from parent cell line).
CC   Genetic integration: Method=Transfection; Gene=FPbase; EGE3L; DsRed (Note=drFP583, red fluorescent protein from Discosoma sp.).
CC   Genetic integration: Method=Transfection; Gene=FPbase; R9NL8; eGFP (Note=Enhanced GFP).
CC   Genetic integration: Method=Transfection; Gene=HGNC; HGNC:13352; MAP1LC3B.
CC   Genetic integration: Method=Transfection; Gene=UniProtKB; P17493; Streptoalloteichus hindustanus ble (BleoR).
CC   Derived from site: In situ; Peripheral blood; UBERON=UBERON_0000178.
DI   NCIt; C9163; Childhood acute monocytic leukemia
DI   ORDO; Orphanet_514; Acute monoblastic/monocytic leukemia
OX   NCBI_TaxID=9606; ! Homo sapiens (Human)
HI   CVCL_X601 ! THP1-Null
SX   Male
AG   1Y
CA   Cancer cell line
DT   Created: 20-05-21; Last updated: 10-04-25; Version: 9
//