ID   293-Dual hSTING-R232
AC   CVCL_A8BN
DR   InvivoGen; 293d-r232
DR   Wikidata; Q107113190
CC   Characteristics: Stably expresses two reporter genes: SEAP (secreted ALPP) and the Lucia luciferase. ALPP expression is under the control of an ISG54 minimal promoter in conjunction with 5 IFN-stimulated response elements (ISRE). The Lucia luciferase reporter gene expression depends on the activation of the endogenous IFNB1 promoter as the IFNB1 gene has been replaced by Lucia (InvivoGen=293d-r232).
CC   Knockout cell: Method=Homologous recombination; HGNC; HGNC:5434; IFNB1.
CC   Genetic integration: Method=Homologous recombination; Gene=Lucia luciferase (Note=Synthetic gene designed on natural secreted luciferase genes from marine copepods).
CC   Genetic integration: Method=Transfection; Gene=HGNC; HGNC:439; ALPP.
CC   Genetic integration: Method=Transfection; Gene=HGNC; HGNC:27962; STING1.
CC   Genetic integration: Method=Transfection; Gene=UniProtKB; P33967; B.cereus Bsr.
CC   Genetic integration: Method=Transfection; Gene=UniProtKB; P00557; Escherichia coli hygromycin-B 4-O-kinase (hph) (HygR).
CC   Genetic integration: Method=Transfection; Gene=UniProtKB; P17493; Streptoalloteichus hindustanus ble (BleoR).
CC   Transformant: NCBI_TaxID; 28285; Adenovirus 5.
CC   Derived from site: In situ; Fetal kidney; UBERON=UBERON_0002113.
OX   NCBI_TaxID=9606; ! Homo sapiens (Human)
HI   CVCL_A8BK ! 293-Dual Null
SX   Female
AG   Fetus
CA   Transformed cell line
DT   Created: 20-05-21; Last updated: 10-04-25; Version: 10
//