ID   Vero-Luc2p
AC   CVCL_A7UI
DR   BEI_Resources; NR-10385
DR   Wikidata; Q107117399
RX   PubMed=20585113;
CC   Group: Non-human primate cell line.
CC   Biotechnology: Used in a quantitative and sensitive luciferase-based assay for bacterial toxins that inhibit protein synthesis (PubMed=20585113).
CC   Characteristics: Stably expresses luciferase Luc2p which is tagged with a PEST sequence which promotes rapid turnover. Therefore, luciferase activity reflects the current level of protein synthesis (PubMed=20585113).
CC   Characteristics: Transduced with a Luc2P-IRES-eGFP construct.
CC   Genetic integration: Method=Transduction; Gene=FPbase; R9NL8; eGFP (Note=Enhanced GFP).
CC   Genetic integration: Method=Transduction; Gene=UniProtKB; P08659; Photinus pyralis firefly-type luciferase (Note=With optimized codon usage for mammalian expression = Luc2).
CC   Derived from site: In situ; Kidney, epithelium; UBERON=UBERON_0004819.
CC   Cell type: Epithelial cell of kidney; CL=CL_0002518.
OX   NCBI_TaxID=60711; ! Chlorocebus sabaeus (Green monkey)
HI   CVCL_0059 ! Vero
SX   Female
AG   Adult
CA   Spontaneously immortalized cell line
DT   Created: 20-05-21; Last updated: 14-08-25; Version: 8
//
RX   PubMed=20585113; DOI=10.1128/AAC.01783-09; PMCID=PMC2935008;
RA   McGannon, Colleen Marie
RA   Fuller, Cynthia Ann
RA   Weiss, Alison Ann
RT   "Different classes of antibiotics differentially influence shiga toxin
RT   production.";
RL   Antimicrob. Agents Chemother. 54:3790-3798(2010).
//