ID   Vero-Luc2p
DR   BEI_Resources; NR-10385
DR   Wikidata; Q107117399
RX   PubMed=20585113;
CC   Group: Non-human primate cell line.
CC   Biotechnology: Used in a quantitative and sensitive luciferase-based assay for bacterial toxins that inhibit protein synthesis (PubMed=20585113).
CC   Characteristics: Stably expresses luciferase Luc2p which is tagged with a PEST sequence which promotes rapid turnover. Therefore, luciferase activity reflects the current level of protein synthesis (PubMed=20585113).
CC   Characteristics: Transduced with a Luc2P-IRES-eGFP construct.
CC   Transfected with: UniProtKB; P08659; Firefly luciferase (with optimized codon usage for mammalian expression).
CC   Transfected with: UniProtKB; P42212; GFP (with p.Phe64Leu, p.Ser65Thr and p.His231Leu = EGFP).
CC   Derived from site: In situ; Kidney, epithelium; UBERON=UBERON_0004819.
CC   Cell type: Epithelial cell of kidney; CL=CL_0002518.
OX   NCBI_TaxID=60711; ! Chlorocebus sabaeus (Green monkey)
HI   CVCL_0059 ! Vero
SX   Female
AG   Adult
CA   Spontaneously immortalized cell line
DT   Created: 20-05-21; Last updated: 05-10-23; Version: 5
RX   PubMed=20585113; DOI=10.1128/AAC.01783-09;
RA   McGannon C.M., Fuller C.A., Weiss A.A.;
RT   "Different classes of antibiotics differentially influence shiga toxin
RT   production.";
RL   Antimicrob. Agents Chemother. 54:3790-3798(2010).