ID   CFS414_1G10
AC   CVCL_A7PF
SY   CFS414-pMB952 clone 1G10; CFS414_1G10-952; CFS414_FLEx-SaCas9-P2A-mCherry
DR   Wikidata; Q107114643
RX   PubMed=35288582;
CC   Group: Bird cell line.
CC   Characteristics: Constitutively expresses nuclear GFP (nGFP) and pac and conditionally expresses S.aureus Cas9 and mCherry in the presence of Cre recombinase.
CC   Characteristics: A FLEx-SaCas9-P2A-mCherry construct was transferred using a PiggyBac transposon vector.
CC   Genetic integration: Method=PiggyBac transposition; Gene=FPbase; ZERB6; mCherry (Note=Red fluorescent protein derived from drFP583 (dsRed)).
CC   Genetic integration: Method=PiggyBac transposition; Gene=UniProtKB; J7RUA5; Staphylococcus aureus Cas9.
CC   Genetic integration: Method=Transduction; Gene=FPbase; R9NL8; eGFP (Note=Enhanced GFP).
CC   Genetic integration: Method=Transduction; Gene=UniProtKB; P13249; S.alboniger pac (PuroR).
CC   Genetic integration: Method=Transduction; Gene=UniProtKB; P03070; SV40 large T antigen.
CC   Transformant: NCBI_TaxID; 1891767; Simian virus 40 (SV40).
CC   Derived from site: In situ; Embryo; UBERON=UBERON_0000922.
CC   Cell type: Fibroblast; CL=CL_0000057.
CC   Breed/subspecies: Continental Chestnut Flanked White; VBO=VBO_0017485.
OX   NCBI_TaxID=59729; ! Taeniopygia guttata (Zebra finch)
HI   CVCL_A7AT ! CFS414
SX   Male
AG   Hamburger-Hamilton stage 28 embryo
CA   Transformed cell line
DT   Created: 20-05-21; Last updated: 10-04-25; Version: 8
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RX   PubMed=35288582; DOI=10.1038/s41598-022-07434-7; PMCID=PMC8921232;
RA   Biegler M.T., Fedrigo O., Collier P., Mountcastle J., Haase B.,
RA   Tilgner H.U., Jarvis E.D.;
RT   "Induction of an immortalized songbird cell line allows for gene
RT   characterization and knockout by CRISPR-Cas9.";
RL   Sci. Rep. 12:4369.1-4369.17(2022).
//