ID   46C-Dip2c KO
AC   CVCL_A7IR
SY   IBMSe001-B-1
DR   Wikidata; Q107113284
RX   PubMed=33813174;
CC   Characteristics: Using CRISPR/Cas9 exon 8 of Dip2c was replaced by a PGKp-PuroR-P2A-mCherry construct (PubMed=33813174).
CC   Characteristics: Using homologous recombination the Sox1 reading frame was replaced by a GFP-IRES-PuroR construct. Expresses eGFP under the control of the Sox-1 promoter (from parent cell line).
CC   Knockout cell: Method=CRISPR/Cas9; MGI; MGI:1920179; Dip2c.
CC   Selected for resistance to: ChEBI; CHEBI_9555; Tioguanine (6-thioguanine; 6-TG).
CC   Genetic integration: Method=CRISPR/Cas9; Gene=FPbase; R9NL8; eGFP (Note=Enhanced GFP).
CC   Genetic integration: Method=CRISPR/Cas9; Gene=FPbase; ZERB6; mCherry (Note=Red fluorescent protein derived from drFP583 (dsRed)).
CC   Genetic integration: Method=Homologous recombination; Gene=FPbase; R9NL8; eGFP (Note=Enhanced GFP).
CC   Genetic integration: Method=Homologous recombination; Gene=UniProtKB; P13249; S.alboniger pac (PuroR).
CC   Derived from site: In situ; Blastocyst; UBERON=UBERON_0000358.
CC   Cell type: Embryonic stem cell; CL=CL_0002322.
CC   Breed/subspecies: 129P2/Ola.
OX   NCBI_TaxID=10090; ! Mus musculus (Mouse)
HI   CVCL_Y482 ! 46C
SX   Male
AG   Blastocyst stage
CA   Embryonic stem cell
DT   Created: 20-05-21; Last updated: 19-12-24; Version: 6
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RX   PubMed=33813174; DOI=10.1016/j.scr.2021.102236;
RA   Yao M.-Z., Su P.-F., Li Z.-F., Cui X.-G., Yang Q., Xing X.-Y., Lei Y.,
RA   Lian M.-Q., Ren T.-L., Wang X.-D., Zheng Y.-W., Wu C.-X.;
RT   "Knockout of Dip2c in murine ES cell line IBMSe001-B-1 by CRISPR/Cas9
RT   genome editing technology.";
RL   Stem Cell Res. 53:102236-102236(2021).
//