ID   WAe001-A-58
AC   CVCL_A7IJ
SY   H1-SARS-CoV-2-N-Teton; GIBHe007-A-58
DR   hPSCreg; WAe001-A-58
DR   Wikidata; Q107117412
RX   PubMed=33771478;
CC   Group: SARS-CoV-2 research cell line.
CC   Characteristics: Transduced with a pB-TRE-[SARS-CoV-2-N]-EF1a-rtTA-2A-puroR-2A-EGFP construct. In the presence of doxycycline rtTA undergo conformational change, binds to the TRE and activates SARS-CoV-2 N protein expression (PubMed=33771478).
CC   Genetic integration: Method=Transduction; Gene=FPbase; 8DNLG; YFP (Note=EYFP, yellow fluorescent protein derived from GFP).
CC   Genetic integration: Method=Transduction; Gene=UniProtKB; P04483; Escherichia coli Tn10 tetR.
CC   Genetic integration: Method=Transduction; Gene=UniProtKB; P06492; Human herpesvirus 1 (HHV-1) VP16 (UL28) (Note=With p.Met1_Arg360del).
CC   Genetic integration: Method=Transduction; Gene=UniProtKB; P0DTC9; Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) nucleoprotein (N).
CC   Genetic integration: Method=Transduction; Gene=UniProtKB; P13249; Streptomyces alboniger pac (PuroR).
CC   Derived from site: In situ; Blastocyst; UBERON=UBERON_0000358.
CC   Cell type: Embryonic stem cell; CL=CL_0002322.
OX   NCBI_TaxID=9606; ! Homo sapiens (Human)
HI   CVCL_9771 ! WA01
SX   Male
AG   Blastocyst stage
CA   Embryonic stem cell
DT   Created: 20-05-21; Last updated: 14-08-25; Version: 9
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RX   PubMed=33771478; DOI=10.1016/j.scr.2021.102197; PMCID=PMC7835078;
RA   Wu, Fei-Ma
RA   Chen, Yan
RA   You, Kai
RA   Tan, Sheng-Lin
RA   Xu, Ying-Ying
RA   Wang, Peng-Hui
RA   Liu, Shi-Ming
RA   Li, Yin-Xiong
RT   "Generation of WAe001-A-58 human embryonic stem cell line with
RT   inducible expression of the SARS-CoV-2 nucleocapsid protein.";
RL   Stem Cell Res. 53:102197-102197(2021).
//