ID   HeLa/Fucci(SA)5
AC   CVCL_A7AV
SY   HeLa/Fucci(SA)5 #16; HeLa/Fucci(SA)5#16
DR   RCB; RCB4917
DR   Wikidata; Q107115325
RX   DOI=10.1101/2020.03.30.015156;
CC   Population: African American.
CC   Characteristics: Used for study of cell cycle progression thanks to the inclusion of a Fucci(SA5) (Fluorescent Ubiquitination-based Cell Cycle Indicator) construct.
CC   Characteristics: Transfected with the Fucci(SA)5 probe which consist of the fusion of AzaleaB5 to the ubiquitination domain of human CDT1 (AA 30-120) and of h2-3 to the ubiquitination domain of human geminin (AA 1-110).
CC   Genetic integration: Method=Transfection; Gene=HGNC; 24576; CDT1 (Note=With p.Met1_Thr29del and p.Ser121_Leu546del).
CC   Genetic integration: Method=Transfection; Gene=HGNC; 17493; GMNN (Note=With p.Tyr111_Ile209del).
CC   Genetic integration: Method=Transfection; Gene=FPbase; 886AH; AzaleaB5 (Note=Red fluorescent protein from Montipora monasteriata).
CC   Genetic integration: Method=Transfection; Gene=FPbase; YQ25V; h2-3 (Note=Bright green/yellow fluorescent protein from Ricordea sp. FP2-3).
CC   Transformant: NCBI_TaxID; 333761; Human papillomavirus type 18 (HPV18).
CC   Derived from site: In situ; Uterus, cervix; UBERON=UBERON_0000002.
DI   NCIt; C27677; Human papillomavirus-related cervical adenocarcinoma
OX   NCBI_TaxID=9606; ! Homo sapiens (Human)
HI   CVCL_0058 ! HeLa S3
SX   Female
AG   30Y6M
CA   Cancer cell line
DT   Created: 20-05-21; Last updated: 10-09-24; Version: 7
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RX   DOI=10.1101/2020.03.30.015156;
RA   Ando R., Sakaue-Sawano A., Shoda K., Miyawaki A.;
RT   "Two new coral fluorescent proteins of distinct colors for sharp
RT   visualization of cell-cycle progression.";
RL   bioRxiv 2020:03.30.015156-03.30.015156(2020).
//