ID   SWI3a-26
AC   CVCL_A5ZP
DR   Wikidata; Q107116990
RX   PubMed=22174749;
RX   PubMed=25804355;
CC   Biotechnology: Cell line for recombinase mediated cassette exchange (RMCE) that was generated by random integration of a FRT-flanked GFP reporter. It contains a single copy of the GFP reporter transgene at a genetic locus that is protected from silencing. The integrated GFP cassette contains a selection trap that allows for selection of recombinant cells upon RMCE (PubMed=25804355).
CC   Characteristics: Transduced with the pEFFS-EGFP-dneo vector that contains eGFP under the control of the EF1a promoter. The eGFP gene is flanked by a wild type FRT site and a synthetic variant (F3). A selection trap, an ATG-deleted, promoterless neo (Dneo) gene is located downstream of eGFP. When eGFP is exchanged by Flp-mediated recombination against a cassette including a promoter and start codon, Dneo becomes complemented and the cell becomes resistant against the antibiotic G418, thus allowing for selection of recombinant cells (PubMed=22174749).
CC   Genetic integration: Method=Transduction; Gene=FPbase; R9NL8; eGFP (Note=Enhanced GFP).
CC   Genetic integration: Method=Transduction; Gene=UniProtKB; P00552; Transposon Tn5 neo.
CC   Derived from site: In situ; Ovary; UBERON=UBERON_0000992.
CC   Cell type: Epithelial cell of ovary; CL=CL_2000064.
OX   NCBI_TaxID=10029; ! Cricetulus griseus (Chinese hamster)
HI   CVCL_K173 ! Lec3.2.8.1
SX   Female
AG   Adult
CA   Spontaneously immortalized cell line
DT   Created: 20-05-21; Last updated: 10-09-24; Version: 7
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RX   PubMed=22174749; DOI=10.1371/journal.pone.0027829; PMCID=PMC3235087;
RA   Wilke S., Groebe L., Maffenbeier V., Jager V., Gossen M., Josewski J.,
RA   Duda A., Polle L., Owens R.J., Wirth D., Heinz D.W.,
RA   van den Heuvel J., Bussow K.;
RT   "Streamlining homogeneous glycoprotein production for biophysical and
RT   structural applications by targeted cell line development.";
RL   PLoS ONE 6:e27829.1-e27829.8(2011).
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RX   PubMed=25804355; DOI=10.1016/j.sbi.2015.03.002;
RA   Bussow K.;
RT   "Stable mammalian producer cell lines for structural biology.";
RL   Curr. Opin. Struct. Biol. 32:81-90(2015).
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