ID   S2R+-Pten-KO-1A4
AC   CVCL_A5UJ
SY   S2R+-Pten KO-1A4
DR   DGRC; 307
DR   FlyBase_Cell_line; FBtc0000307
DR   Wikidata; Q107116825
CC   Group: Insect cell line.
CC   Characteristics: Transfected with a pMT-FLAG-Cas9 construct where the expression of N-terminally FLAG-tagged Cas9 is driven by a copper-inducible metallothionein promoter (from parent cell line).
CC   Knockout cell: Method=CRISPR/Cas9; FlyBase_Gene; FBgn0026379; Pten.
CC   Genetic integration: Method=Protein trap; Gene=FPbase; ZERB6; mCherry (Note=Red fluorescent protein derived from drFP583 (dsRed)).
CC   Genetic integration: Method=Transfection; Gene=UniProtKB; P00557; Escherichia coli hygromycin-B 4-O-kinase (hph) (HygR).
CC   Genetic integration: Method=Transfection; Gene=UniProtKB; Q99ZW2; Streptomyces pyogenes Cas9 (Note=With a N-termial FLAG tag).
CC   Derived from site: In situ; Whole embryo; UBERON=UBERON_0000922.
CC   Breed/subspecies: Oregon-R; FlyBase_Strain=FBsn0000276.
OX   NCBI_TaxID=7227; ! Drosophila melanogaster (Fruit fly)
HI   CVCL_UD30 ! S2R+-MT::Cas9
SX   Male
AG   Late embryonic stage
CA   Spontaneously immortalized cell line
DT   Created: 20-05-21; Last updated: 14-08-25; Version: 7
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