ID   FM3A G258
AC   CVCL_A5LA
SY   G258
DR   Wikidata; Q107115030
RX   PubMed=1995072;
RX   PubMed=6725415;
RX   PubMed=10410677;
CC   Characteristics: Temperature-sensitive. Deficient in cell growth, asparagine glycosylation and synthesis of oligosaccharide-lipid. Selection is effected by tritium suicide selection. Mutagenized cells are bathed in tritiated mannose and stored so as to allow time for cells that had taken up the mannose to be killed by radiation damage (PubMed=1995072; PubMed=6725415).
CC   Transformant: ChEBI; CHEBI:21759; N-methyl-N'-nitro-N-nitrosoguanidine (MNNG).
CC   Derived from site: In situ; Mammary gland; UBERON=UBERON_0001911.
CC   Breed/subspecies: C3H/He.
DI   NCIt; C21678; Malignant neoplasms of the mouse mammary gland
OX   NCBI_TaxID=10090; ! Mus musculus (Mouse)
HI   CVCL_D687 ! FM3A F28-7
SX   Female
CA   Cancer cell line
DT   Created: 20-05-21; Last updated: 29-06-23; Version: 4
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RX   PubMed=1995072; DOI=10.1016/0167-4889(91)90052-y;
RA   Nishikawa Y.;
RT   "An FM3A mutant, G258, with a mutation that affects both cell growth
RT   and oligosaccharide-lipid synthesis.";
RL   Biochim. Biophys. Acta 1091:135-140(1991).
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RX   PubMed=6725415; DOI=10.1002/jcp.1041190303;
RA   Nishikawa Y.;
RT   "Isolation of a temperature-sensitive FM3A mutant deficient in
RT   asparagine-linked glycosylation by selecting for resistance to
RT   tritiated mannose suicide.";
RL   J. Cell. Physiol. 119:260-266(1984).
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RX   PubMed=10410677; DOI=10.1023/B:SCAM.0000007125.41715.8d;
RA   Kataoka K., Takahashi T., Ayusawa D., Nishikawa Y.;
RT   "Characterization of a human genomic DNA fragment which rescues
RT   defective lipid-linked oligosaccharide synthesis in a mutant G258 cell
RT   line isolated from the FM3A mouse mammary carcinoma cell line.";
RL   Somat. Cell Mol. Genet. 24:235-243(1998).
//