ID   TEX
AC   CVCL_A5CF
DR   BTO; BTO_0006359
DR   GEO; GSM838425
DR   GEO; GSM838426
DR   GEO; GSM838427
DR   Wikidata; Q107117030
RX   PubMed=16094415;
RX   PubMed=22160482;
CC   Characteristics: Expresses a transfected FUS-ERG (TLS-ERG) fusion protein. Mimics the features of primary human acute myeloid leukemia and of leukemia-initiating cells (LIC) and are more than 90% CD34+. Underwent step-wise transformation and immortalization through acquisition of additional genetic changes, such as expression of TERT, expression of LIC markers CD34, CD44 and CD123 and the stem cell gene BMI1 (PubMed=16094415; PubMed=22160482).
CC   Genetic integration: Method=Transfection/transduction; Gene=HGNC; HGNC:3446; ERG (Note=FUS-ERG fusion).
CC   Genetic integration: Method=Transfection/transduction; Gene=HGNC; HGNC:4010; FUS (Note=FUS-ERG fusion).
CC   Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; P00552; Transposon Tn5 neo.
CC   Omics: Transcriptomics; Microarray.
CC   Derived from site: In situ; Umbilical cord blood; UBERON=UBERON_0012168.
OX   NCBI_TaxID=9606; ! Homo sapiens (Human)
SX   Sex unspecified
AG   <1D
CA   Transformed cell line
DT   Created: 20-05-21; Last updated: 10-04-25; Version: 8
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RX   PubMed=16094415; DOI=10.1038/sj.leu.2403917;
RA   Warner J.K., Wang J.C.Y., Takenaka K., Doulatov S., McKenzie J.L.,
RA   Harrington L., Dick J.E.;
RT   "Direct evidence for cooperating genetic events in the leukemic
RT   transformation of normal human hematopoietic cells.";
RL   Leukemia 19:1794-1805(2005).
//
RX   PubMed=22160482; DOI=10.1182/blood-2011-01-330019;
RA   McDermott S.P., Eppert K., Notta F., Isaac M., Datti A., Al-awar R.,
RA   Wrana J.L., Minden M.D., Dick J.E.;
RT   "A small molecule screening strategy with validation on human leukemia
RT   stem cells uncovers the therapeutic efficacy of kinetin riboside.";
RL   Blood 119:1200-1207(2012).
//