ID   SCSe001-A-3
AC   CVCL_A4WX
SY   Mel1 INS-GFP SST-RFP; CHOPe002-A-3
DR   hPSCreg; SCSe001-A-3
DR   Wikidata; Q107116863
RX   PubMed=33316598;
CC   From: Children's Hospital of Philadelphia; Philadelphia; USA.
CC   Characteristics: Using homologous recombination an EGFP reporter gene was introduced in the INS locus and using CRISPR/Cas9 a tandem-dimer RFP was introduced in the SST gene (PubMed=33316598).
CC   Transfected with: UniProtKB; Q9U6Y8; Discosoma sp. red fluorescent protein drFP583 (DsRed, RFP).
CC   Transfected with: UniProtKB; P42212; GFP (with p.Phe64Leu, p.Ser65Thr and p.His231Leu = EGFP).
CC   Derived from site: In situ; Blastocyst; UBERON=UBERON_0000358.
CC   Cell type: Embryonic stem cell; CL=CL_0002322.
OX   NCBI_TaxID=9606; ! Homo sapiens (Human)
HI   CVCL_XA16 ! MEL-1 INSGFP/w
SX   Male
AG   Blastocyst stage
CA   Embryonic stem cell
DT   Created: 20-05-21; Last updated: 05-10-23; Version: 5
//
RX   PubMed=33316598; DOI=10.1016/j.scr.2020.102112;
RA   Leavens K.F., Liao C.-M., Gagne A.L., Kishore S., Cardenas-Diaz F.L.,
RA   French D.L., Gadue P.;
RT   "Generation of a double insulin and somatostatin reporter line,
RT   SCSe001-A-3, for the advancement of stem cell-derived pancreatic
RT   islets.";
RL   Stem Cell Res. 50:102112-102112(2021).
//