ID   MCF 10A Ecadherin EmGFP
AC   CVCL_A4BW
SY   MCF 10A ECAD-EmGFP
DR   ATCC; CRL-10317EMT
DR   Wikidata; Q105510065
CC   Characteristics: Using CRISPR/Cas9 CDH1 has been tagged in one allele at the C-terminus with EmGFP. Allows to monitor the epithelial-to-mesenchymal transition (EMT) status of cells in vitro by monitoring EmGFP expression.
CC   Sequence variation: Gene deletion; HGNC; HGNC:1787; CDKN2A; Zygosity=Homozygous (from parent cell line).
CC   Sequence variation: Gene deletion; HGNC; HGNC:1788; CDKN2B; Zygosity=Homozygous (from parent cell line).
CC   Genetic integration: Method=CRISPR/Cas9; Gene=FPbase; GLAD6; Emerald (Note=EmGFP, green fluorescent protein derived from GFP).
CC   Derived from site: In situ; Breast, epithelium; UBERON=UBERON_0008367.
ST   Source(s): ATCC=CRL-10317EMT
ST   Amelogenin: X
ST   CSF1PO: 10,12
ST   D13S317: 8,9
ST   D16S539: 11,12
ST   D5S818: 10,13
ST   D7S820: 10,11
ST   TH01: 8,9.3
ST   TPOX: 9,11
ST   vWA: 15,17
OX   NCBI_TaxID=9606; ! Homo sapiens (Human)
HI   CVCL_0598 ! MCF-10A
SX   Female
AG   36Y
CA   Spontaneously immortalized cell line
DT   Created: 12-01-21; Last updated: 19-12-24; Version: 10
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