ID   EndoC-betaH21-PGF2AOF
AC   CVCL_A1TV
DR   Wikidata; Q105506836
RX   PubMed=25259951;
CC   Characteristics: The lentiviral vector insert containing the SV40 LT and hTERT can be excised by the Cre recombinase upon addition of tamixofen. Following excision of immortalizing transgenes, cell proliferation sharply decreases and in parallel there is a massive enhancement of beta cell-specific features such as increased INS gene expression.
CC   Characteristics: Transduced with the Fucci probe which consist of the fusion of monomeric Kusabira-Orange2 (mKO2) to the ubiquitination domain of human CDT1 (AA 30-120) and monomeric AzamiGreen (mAG) to the ubiquitination domain of human geminin (AA 1-110).
CC   Characteristics: In this cell line the Fucci probe was produced by transducing with a construct LTR-PuroR-IRES-mAG/GMNN-2A-mKO2/CDT1-LTR.
CC   Virology: Contains an integrated xenotropic MuLV-related virus (XMRV) Bxv-1 (from parent cell line).
CC   Genetic integration: Method=Transduction; Gene=FPbase; HC91W; AzamiGreen (Note=Green fluorescent protein from G.fascicularis).
CC   Genetic integration: Method=Transduction; Gene=FPbase; 134NN; mKusabira-Orange2 (Note=mKO2, orange fluorescent protein derived from F.concinna Kusabira-Orange (KO)).
CC   Genetic integration: Method=Transduction; Gene=HGNC; HGNC:24576; CDT1 (Note=With p.Met1_Thr29del and p.Ser121_Leu546del).
CC   Genetic integration: Method=Transduction; Gene=HGNC; HGNC:17493; GMNN (Note=With p.Tyr111_Ile209del).
CC   Genetic integration: Method=Transduction; Gene=HGNC; HGNC:11730; TERT.
CC   Genetic integration: Method=Transduction; Gene=MGI; MGI:1352467; Esr1 (Note=Ligand binding domain; with p.Gly525Arg. Fused to Cre = CreERT2).
CC   Genetic integration: Method=Transduction; Gene=UniProtKB; P06956; Escherichia phage P1 cre (Note=Fused to ligand domain of mouse Esr1 = CreERT2).
CC   Genetic integration: Method=Transduction; Gene=UniProtKB; P13249; S.alboniger pac (PuroR).
CC   Genetic integration: Method=Transduction; Gene=UniProtKB; P03070; SV40 large T antigen.
CC   Transformant: NCBI_TaxID; 1891767; Simian virus 40 (SV40).
CC   Derived from site: In situ; Fetal pancreas, islets of Langerhans; UBERON=UBERON_0000006.
CC   Cell type: Pancreatic beta cell; CL=CL_0000169.
OX   NCBI_TaxID=9606; ! Homo sapiens (Human)
HI   CVCL_IS71 ! EndoC-betaH2
SX   Sex unspecified
AG   9FW
CA   Conditionally immortalized cell line
DT   Created: 12-01-21; Last updated: 10-04-25; Version: 11
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RX   PubMed=25259951; DOI=10.1371/journal.pone.0108202; PMCID=PMC4178124;
RA   Carlier G., Maugein A., Cordier C., Pechberty S., Garfa-Traore M.,
RA   Martin P., Scharfmann R., Albagli-Curiel O.;
RT   "Human Fucci pancreatic beta cell lines: new tools to study beta cell
RT   cycle and terminal differentiation.";
RL   PLoS ONE 9:e108202.1-e108202.14(2014).
//