ID   HeLa-SHMT2-shSHMT2
AC   CVCL_A1AR
SY   HeLa-Ss
DR   MetaboLights; MTBLS405
DR   PRIDE; PXD004629
DR   PRIDE; PXD004954
DR   Wikidata; Q102114111
RX   PubMed=32903271;
CC   Population: African American.
CC   Characteristics: Allows both a Tet-inducible over-expression of mouse Shmt2 and isopropyl beta-D-1-thiogalactopyranoside (IPTG)-inducible shRNA against SHMT2.
CC   Knockout cell: Method=shRNA knockdown; HGNC; HGNC:10852; SHMT2.
CC   Genetic integration: Method=Transfection/transduction; Gene=MGI; MGI:1277989; Shmt2.
CC   Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; P00557; Escherichia coli hygromycin-B 4-O-kinase (hph) (HygR).
CC   Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; P04483; Escherichia coli Tn10 tetR.
CC   Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; P13249; S.alboniger pac (PuroR).
CC   Transformant: NCBI_TaxID; 333761; Human papillomavirus type 18 (HPV18).
CC   Derived from site: In situ; Uterus, cervix; UBERON=UBERON_0000002.
DI   NCIt; C27677; Human papillomavirus-related endocervical adenocarcinoma
OX   NCBI_TaxID=9606; ! Homo sapiens (Human)
HI   CVCL_D587 ! T-REx-HeLa
SX   Female
AG   30Y6M
CA   Cancer cell line
DT   Created: 29-10-20; Last updated: 10-04-25; Version: 10
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RX   PubMed=32903271; DOI=10.1371/journal.pone.0237981; PMCID=PMC7480864;
RA   Tong J.-F., Krieger J.R., Taylor P., Bagshaw R.D., Kang J., Jeedigunta S.,
RA   Wybenga-Groot L.E., Zhang W., Badr H., Mirhadi S., Pham N.-A.,
RA   Coyaud E., Yu M., Li M., Cabanero M., Raught B., Maynes J.T.,
RA   Hawkins C., Tsao M.-S., Moran M.F.;
RT   "Cancer proteome and metabolite changes linked to SHMT2.";
RL   PLoS ONE 15:e0237981.1-e0237981.25(2020).
//