ID   CHO MGAT1- TZ97008-rgp120 3E5
AC   CVCL_A0BU
SY   3E5 MGAT1- CHO TZ97008
DR   Wikidata; Q108820033
RX   PubMed=30344523;
CC   Group: Serum/protein free medium cell line.
CC   Characteristics: Produces a gp120 from a clade C isolate (TZ97008-rgp120) and enriched for mannose-5 glycans. The gp120 construct contains an N-terminal purification tag from HSV-1 glycoprotein D (gD).
CC   Knockout cell: Method=CRISPR/Cas9; UniProtKB; Q924C0; Mgat1.
CC   Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; P00552; Transposon Tn5 neo.
CC   Genetic integration: Method=Transfection/transduction; Gene=HIV-1 isolate TZ97008 env (gp120).
CC   Derived from site: In situ; Ovary; UBERON=UBERON_0000992.
CC   Cell type: Epithelial cell of ovary; CL=CL_2000064.
OX   NCBI_TaxID=10029; ! Cricetulus griseus (Chinese hamster)
HI   CVCL_VP59 ! CHO MGAT1-
SX   Female
AG   Adult
CA   Spontaneously immortalized cell line
DT   Created: 23-09-21; Last updated: 10-04-25; Version: 7
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RX   PubMed=30344523; DOI=10.3389/fimmu.2018.02313; PMCID=PMC6182045;
RA   Doran R.C., Yu B., Wright M., O'Rourke S.M., Yin L., Richardson J.M.,
RA   Byrne G., Mesa K.A., Berman P.W.;
RT   "Development of a stable MGAT1(-) CHO cell line to produce clade C
RT   gp120 with improved binding to broadly neutralizing antibodies.";
RL   Front. Immunol. 9:2313.1-2313.13(2018).
//