ID   HepaRG
AC   CVCL_9720
SY   Hepa-RG
DR   BTO; BTO_0005736
DR   EFO; EFO_0001186
DR   ChEMBL-Cells; CHEMBL4630773
DR   ChEMBL-Targets; CHEMBL4630781
DR   Cosmic; 2162524
DR   MetaboLights; MTBLS78
DR   MetaboLights; MTBLS4063
DR   Millipore; C103485
DR   PRIDE; PXD001107
DR   PRIDE; PXD004752
DR   PRIDE; PXD006267
DR   PRIDE; PXD010304
DR   PubChem_Cell_line; CVCL_9720
DR   Wikidata; Q54882834
RX   Patent=US7456018;
RX   PubMed=12432097;
RX   PubMed=17241619;
RX   PubMed=17393521;
RX   PubMed=20228232;
RX   PubMed=20645056;
RX   PubMed=21414303;
RX   PubMed=22568886;
RX   PubMed=22594799;
RX   PubMed=22643240;
RX   PubMed=22857383;
RX   PubMed=23887712;
RX   PubMed=26160117;
RX   PubMed=26694163;
RX   PubMed=27027780;
RX   PubMed=27169750;
RX   PubMed=27780834;
RX   PubMed=27975304;
RX   PubMed=28904299;
RX   PubMed=30795634;
RX   PubMed=31063779;
RX   PubMed=33441761;
WW   Info; LCCL; -; https://lccl.zucmanlab.com/hcc/cellLines/HepaRG
WW   Info; Wikipedia; -; https://en.wikipedia.org/wiki/HepaRG
WW   Provider; Biopredic; -; https://web.archive.org/web/20221228183303/http://www.heparg.com/
WW   Provider; Biopredic; -; https://www.biopredicadvancells.com/product/cryopreserved-heparg-116-cells/
WW   Provider; Thermo Fisher; HPRGC10; https://www.thermofisher.com/order/catalog/product/HPRGC10
WW   Provider; TRL; -; https://web.archive.org/web/20171123042648/http://triangleresearchlabs.net/products-hepatocytes/nospin-heparg/
CC   Group: Patented cell line.
CC   Registration: International Depositary Authority, Pasteur Institute Collection Nationale de Cultures de Micro-organismes (CNCM); I-2652.
CC   Characteristics: Can be induced to differentiate into hepatocyte-like cells by exposure to DMSO.
CC   Characteristics: Cell line main applications are sugar and lipid metabolism, physiopathology; drug metabolism and toxicology and viral infection.
CC   Doubling time: 24 hours (PubMed=12432097).
CC   Omics: Genomics; Whole exome sequencing.
CC   Omics: Proteomics.
CC   Omics: Proteomics; Expression; Reverse-phase protein array.
CC   Omics: Proteomics; Subcellular; Membrane.
CC   Omics: Proteomics; Subcellular; Secretome.
CC   Omics: Transcriptomics; Microarray.
CC   Omics: Transcriptomics; miRNA profiling; Microarray.
CC   Anecdotal: The 'RG' in the cell name stands for 'Rumin' and 'Gripon', the two scientists that were influential in the establishment of this cell line.
CC   Miscellaneous: This cell line is exclusively produced and controlled by Biopredic International and owned by Inserm. BioPredic sells undifferentiated cell line (HPR101) that can be expanded, passaged, and frozen in-house to prepare a cell bank and differentiated ready-to-use cells, HPR116 (Cellosaurus=CVCL_WL73) that can be used immediately or in a few days after thawing, as a single-use disposable product (Biopredic).
CC   Discontinued: Millipore; C103485; true.
CC   Derived from site: In situ; Liver; UBERON=UBERON_0002107.
DI   NCIt; C3098; Hepatitis C infection
DI   NCIt; C3099; Hepatocellular carcinoma
DI   ORDO; Orphanet_88673; Hepatocellular carcinoma
OX   NCBI_TaxID=9606; ! Homo sapiens (Human)
SX   Female
AG   Age unspecified
CA   Cancer cell line
DT   Created: 06-06-12; Last updated: 27-11-25; Version: 34
CH   CVCL_FA14 ! Hepa-SC
CH   CVCL_B6A6 ! HepaRG 5F
CH   CVCL_JJ68 ! HepaRG CYP3A4G/7R clone 3
CH   CVCL_XI49 ! HepaRG-CAR
CH   CVCL_JY39 ! HepaRG-hNTCP
CH   CVCL_C0QZ ! HepaRG(VC)
CH   CVCL_WL73 ! HPR116
//
RX   PubMed=28904299; DOI=10.2131/jts.42.641;
RA   Tomida, Takafumi
RA   Ishimura, Masakazu
RA   Iwaki, Masahiro
RT   "A cell-based assay using HepaRG cells for predicting drug-induced
RT   phospholipidosis.";
RL   J. Toxicol. Sci. 42:641-650(2017).
//
RX   PubMed=23887712; DOI=10.1038/ncomms3218; PMCID=PMC3731665;
RA   Nault, Jean-Charles
RA   Mallet, Maxime
RA   Pilati, Camilla
RA   Calderaro, Julien
RA   Bioulac-Sage, Paulette
RA   Laurent, Christophe
RA   Laurent, Alexis
RA   Cherqui, Daniel
RA   Balabaud, Charles
RA   Zucman-Rossi, Jessica
RT   "High frequency of telomerase reverse-transcriptase promoter somatic
RT   mutations in hepatocellular carcinoma and preneoplastic lesions.";
RL   Nat. Commun. 4:2218.1-2218.7(2013).
//
RX   PubMed=27169750; DOI=10.1038/srep24709; PMCID=PMC4867683;
RA   Sharanek, Ahmad
RA   Burban, Audrey
RA   Burbank, Matthew
RA   Le Guevel, Remy
RA   Li, Ruo-Ya
RA   Guillouzo, Andre
RA   Guguen-Guillouzo, Christiane
RT   "Rho-kinase/myosin light chain kinase pathway plays a key role in the
RT   impairment of bile canaliculi dynamics induced by cholestatic drugs.";
RL   Sci. Rep. 6:24709.1-24709.18(2016).
//
RX   Patent=US7456018;
RA   Gripon, Philippe
RA   Guguen-Guillouzo, Christiane
RA   Trepo, Christian
RA   Rumin, Sylvie
RT   "Human hepatoma lines, methods for obtaining same and uses thereof.";
RL   Patent number US7456018, 25-Nov-2008.
//
RX   PubMed=26160117; DOI=10.1093/toxsci/kfv136; PMCID=PMC4583060;
RA   Sison-Young, Rowena L.C.
RA   Mitsa, Dimitra
RA   Jenkins, Rosalind E.
RA   Mottram, David
RA   Alexandre, Eliane
RA   Richert, Lysiane
RA   Aerts, Helene
RA   Weaver, Richard J.
RA   Jones, Robert P.
RA   Johann, Esther
RA   Hewitt, Philip G.
RA   Ingelman-Sundberg, Magnus
RA   Goldring, Christopher E.P.
RA   Kitteringham, Neil R.
RA   Park, Brian Kevin
RT   "Comparative proteomic characterization of 4 human liver-derived
RT   single cell culture models reveals significant variation in the
RT   capacity for drug disposition, bioactivation, and detoxication.";
RL   Toxicol. Sci. 147:412-424(2015).
//
RX   PubMed=22857383; DOI=10.1186/1477-5956-10-47; PMCID=PMC3527237;
RA   Sokolowska, Izabela
RA   Dorobantu, Cristina M.
RA   Woods, Alisa G.
RA   Macovei, Alina
RA   Branza-Nichita, Norica
RA   Darie, Costel C.
RT   "Proteomic analysis of plasma membranes isolated from undifferentiated
RT   and differentiated HepaRG cells.";
RL   Proteome Sci. 10:47.1-47.13(2012).
//
RX   PubMed=27027780; DOI=10.1007/s10565-016-9316-2;
RA   Wu, Yu
RA   Geng, Xing-Chao
RA   Wang, Ju-Feng
RA   Miao, Yu-Fa
RA   Lu, Yan-Li
RA   Li, Bo
RT   "The HepaRG cell line, a superior in vitro model to L-02, HepG2 and
RT   hiHeps cell lines for assessing drug-induced liver injury.";
RL   Cell Biol. Toxicol. 32:37-59(2016).
//
RX   PubMed=27780834; DOI=10.1124/dmd.116.072603;
RA   van der Mark, Vincent A.
RA   de Waart, Dirk Rudi
RA   Shevchenko, Valery
RA   Oude Elferink, Ronald P.J.
RA   Chamuleau, Robert A.F.M.
RA   Hoekstra, Ruurdtje
RT   "Stable overexpression of the constitutive androstane receptor reduces
RT   the requirement for culture with dimethyl sulfoxide for high drug
RT   metabolism in HepaRG cells.";
RL   Drug Metab. Dispos. 45:56-67(2017).
//
RX   PubMed=20645056; DOI=10.1007/978-1-60761-688-7_13;
RA   Marion, Marie-Jeanne
RA   Hantz, Olivier
RA   Durantel, David
RT   "The HepaRG cell line: biological properties and relevance as a tool
RT   for cell biology, drug metabolism, and virology studies.";
RL   Methods Mol. Biol. 640:261-272(2010).
//
RX   PubMed=31063779; DOI=10.1053/j.gastro.2019.05.001;
RA   Caruso, Stefano
RA   Calatayud, Anna-Line
RA   Pilet, Jill
RA   La Bella, Tiziana
RA   Rekik, Samia
RA   Imbeaud, Sandrine
RA   Letouze, Eric
RA   Meunier, Lea
RA   Bayard, Quentin
RA   Rohr-Udilova, Nataliya
RA   Peneau, Camille
RA   Grasl-Kraupp, Bettina
RA   de Koning, Leanne
RA   Ouine, Berengere
RA   Bioulac-Sage, Paulette
RA   Couchy, Gabrielle
RA   Calderaro, Julien
RA   Nault, Jean-Charles
RA   Zucman-Rossi, Jessica
RA   Rebouissou, Sandra
RT   "Analysis of liver cancer cell lines identifies agents with likely
RT   efficacy against hepatocellular carcinoma and markers of response.";
RL   Gastroenterology 157:760-776(2019).
//
RX   PubMed=27975304; DOI=10.1007/978-1-4939-6700-1_2;
RA   Ni, Yi
RA   Urban, Stephan
RT   "Hepatitis B virus infection of HepaRG cells, HepaRG-hNTCP cells, and
RT   primary human hepatocytes.";
RL   Methods Mol. Biol. 1540:15-25(2017).
//
RX   PubMed=20228232; DOI=10.1124/dmd.109.031831; PMCID=PMC2879958;
RA   Hart, Steven N.
RA   Li, Ye
RA   Nakamoto, Kaori
RA   Subileau, Eva-Anne
RA   Steen, David
RA   Zhong, Xiao-Bo
RT   "A comparison of whole genome gene expression profiles of HepaRG cells
RT   and HepG2 cells to primary human hepatocytes and human liver
RT   tissues.";
RL   Drug Metab. Dispos. 38:988-994(2010).
//
RX   PubMed=12432097; DOI=10.1073/pnas.232137699; PMCID=PMC137772;
RA   Gripon, Philippe
RA   Rumin, Sylvie
RA   Urban, Stephan
RA   Le Seyec, Jacques
RA   Glaise, Denise
RA   Cannie, Isabelle
RA   Guyomard, Claire
RA   Lucas, Josette
RA   Trepo, Christian
RA   Guguen-Guillouzo, Christiane
RT   "Infection of a human hepatoma cell line by hepatitis B virus.";
RL   Proc. Natl. Acad. Sci. U.S.A. 99:15655-15660(2002).
//
RX   PubMed=21414303; DOI=10.1016/j.bcp.2011.03.004;
RA   Ceelen, Liesbeth
RA   De Spiegelaere, Ward
RA   David, Michael
RA   De Craene, Jurgen
RA   Vinken, Mathieu
RA   Vanhaecke, Tamara
RA   Rogiers, Vera
RT   "Critical selection of reliable reference genes for gene expression
RT   study in the HepaRG cell line.";
RL   Biochem. Pharmacol. 81:1255-1261(2011).
//
RX   PubMed=30795634; DOI=10.3390/cells8020192; PMCID=PMC6406872;
RA   Tascher, Georg
RA   Burban, Audrey
RA   Camus, Sandrine
RA   Plumel, Marine I.
RA   Chanon, Stephanie
RA   Le Guevel, Remy
RA   Shevchenko, Valery
RA   Van Dorsselaer, Alain
RA   Lefai, Etienne
RA   Guguen-Guillouzo, Christiane
RA   Bertile, Fabrice
RT   "In-depth proteome analysis highlights HepaRG cells as a versatile
RT   cell system surrogate for primary human hepatocytes.";
RL   Cells 8:192.1-192.25(2019).
//
RX   PubMed=17241619; DOI=10.1016/j.cbi.2006.12.003;
RA   Guillouzo, Andre
RA   Corlu, Anne
RA   Aninat, Caroline
RA   Glaise, Denise
RA   Morel, Fabrice
RA   Guguen-Guillouzo, Christiane
RT   "The human hepatoma HepaRG cells: a highly differentiated model for
RT   studies of liver metabolism and toxicity of xenobiotics.";
RL   Chem. Biol. Interact. 168:66-73(2007).
//
RX   PubMed=22594799; DOI=10.3109/13813455.2012.683442;
RA   Huaman Samanez, Carolina
RA   Caron, Sandrine
RA   Briand, Olivier
RA   Dehondt, Helene
RA   Duplan, Isabelle
RA   Kuipers, Folkert
RA   Hennuyer, Nathalie
RA   Clavey, Veronique
RA   Staels, Bart
RT   "The human hepatocyte cell lines IHH and HepaRG: models to study
RT   glucose, lipid and lipoprotein metabolism.";
RL   Arch. Physiol. Biochem. 118:102-111(2012).
//
RX   PubMed=22643240; DOI=10.1016/j.tiv.2012.05.008;
RA   Antherieu, Sebastien
RA   Chesne, Christophe
RA   Li, Ruo-Ya
RA   Guguen-Guillouzo, Christiane
RA   Guillouzo, Andre
RT   "Optimization of the HepaRG cell model for drug metabolism and
RT   toxicity studies.";
RL   Toxicol. In Vitro 26:1278-1285(2012).
//
RX   PubMed=17393521; DOI=10.1002/hep.21536;
RA   Cerec, Virginie
RA   Glaise, Denise
RA   Garnier, Delphine
RA   Morosan, Serban
RA   Turlin, Bruno
RA   Drenou, Bernard
RA   Gripon, Philippe
RA   Kremsdorf, Dina
RA   Guguen-Guillouzo, Christiane
RA   Corlu, Anne
RT   "Transdifferentiation of hepatocyte-like cells from the human hepatoma
RT   HepaRG cell line through bipotent progenitor.";
RL   Hepatology 45:957-967(2007).
//
RX   PubMed=33441761; DOI=10.1038/s41598-020-79952-1; PMCID=PMC7806635;
RA   Heintze, Tamara
RA   Klein, Kathrin
RA   Hofmann, Ute
RA   Zanger, Ulrich M.
RT   "Differential effects on human cytochromes P450 by CRISPR/Cas9-induced
RT   genetic knockout of cytochrome P450 reductase and cytochrome b5 in
RT   HepaRG cells.";
RL   Sci. Rep. 11:1000.1-1000.14(2021).
//
RX   PubMed=26694163; DOI=10.1371/journal.pone.0144924; PMCID=PMC4692263;
RA   Janiszewska, Joanna
RA   Szaumkessel, Marcin
RA   Kostrzewska-Poczekaj, Magdalena
RA   Bednarek, Kinga
RA   Paczkowska, Julia
RA   Jackowska, Joanna
RA   Grenman, Reidar
RA   Szyfter, Krzysztof
RA   Wierzbicka, Malgorzata
RA   Giefing, Maciej
RA   Jarmuz-Szymczak, Malgorzata
RT   "Global miRNA expression profiling identifies miR-1290 as novel
RT   potential oncomiR in laryngeal carcinoma.";
RL   PLoS ONE 10:e0144924.1-e0144924.13(2015).
//
RX   PubMed=22568886; DOI=10.1517/17425255.2012.685159;
RA   Andersson, Tommy B.
RA   Kanebratt, Kajsa P.
RA   Kenna, J. Gerry
RT   "The HepaRG cell line: a unique in vitro tool for understanding drug
RT   metabolism and toxicology in human.";
RL   Expert Opin. Drug Metab. Toxicol. 8:909-920(2012).
//