ID   MDA-kb2
AC   CVCL_6421
SY   MDA-Kb2
DR   CLO; CLO_0007630
DR   ATCC; CRL-2713
DR   cancercelllines; CVCL_6421
DR   CLS; 305108
DR   PubChem_Cell_line; CVCL_6421
DR   Wikidata; Q54904576
RX   PubMed=11861974;
RX   PubMed=20821581;
CC   Group: Triple negative breast cancer (TNBC) cell line.
CC   Population: Caucasian.
CC   Biotechnology: Can be used in an vitro assay to screen androgen agonist and antagonists and to characterize its specificity and sensitivity to endocrine disrupting chemicals.
CC   Characteristics: Expresses luciferase under the control of a MMTV promoter that contains glucocorticoid receptor and androgen receptor response elements.
CC   Sequence variation: Mutation; HGNC; 644; AR; Simple; p.Gln868His (c.2604G>T); Zygosity=Unspecified (from parent cell line).
CC   Sequence variation: Mutation; HGNC; 1748; CDH1; Simple; p.Trp638Ter (c.1913G>A); ClinVar=VCV000599651; Zygosity=Homozygous (from parent cell line).
CC   Sequence variation: Mutation; HGNC; 6407; KRAS; Simple; p.Gly13Asp (c.38G>A); ClinVar=VCV000012580; Zygosity=Unspecified (from parent cell line).
CC   Sequence variation: Mutation; HGNC; 8975; PIK3CA; Simple; p.His1047Arg (c.3140A>G); ClinVar=VCV000013652; Zygosity=Heterozygous (from parent cell line).
CC   Sequence variation: Mutation; HGNC; 9588; PTEN; Simple; p.Glu307Lys (c.919G>A); Zygosity=Heterozygous (from parent cell line).
CC   Sequence variation: Mutation; HGNC; 11998; TP53; Unexplicit; Ex10-11del; Zygosity=Homozygous (from parent cell line).
CC   Transfected with: UniProtKB; P08659; Firefly luciferase.
CC   Transfected with: UniProtKB; P00552; Transposon Tn5 neo.
CC   Derived from site: Metastatic; Pericardial effusion; UBERON=UBERON_0002409.
ST   Source(s): ATCC=CRL-2713
ST   Amelogenin: X
ST   CSF1PO: 10,12
ST   D13S317: 12
ST   D16S539: 9
ST   D18S51: 15,20
ST   D19S433: 13,14
ST   D21S11: 29,31
ST   D2S1338: 23,24
ST   D3S1358: 15
ST   D5S818: 11
ST   D7S820: 10
ST   D8S1179: 10,12
ST   FGA: 18,23
ST   Penta D: 9,10
ST   Penta E: 11
ST   TH01: 6
ST   TPOX: 10
ST   vWA: 17,18
DI   NCIt; C5214; Breast adenocarcinoma
OX   NCBI_TaxID=9606; ! Homo sapiens (Human)
HI   CVCL_0418 ! MDA-MB-453
SX   Female
AG   48Y
CA   Cancer cell line
DT   Created: 04-04-12; Last updated: 02-05-24; Version: 25
//
RX   PubMed=11861974; DOI=10.1093/toxsci/66.1.69;
RA   Wilson V.S., Bobseine K., Lambright C.R., Gray L.E. Jr.;
RT   "A novel cell line, MDA-kb2, that stably expresses an androgen- and
RT   glucocorticoid-responsive reporter for the detection of hormone
RT   receptor agonists and antagonists.";
RL   Toxicol. Sci. 66:69-81(2002).
//
RX   PubMed=20821581; DOI=10.1002/etc.166;
RA   Blake L.S., Martinovic D., Gray L.E. Jr., Wilson V.S., Regal R.R.,
RA   Villeneuve D.L., Ankley G.T.;
RT   "Characterization of the androgen-sensitive MDA-kb2 cell line for
RT   assessing complex environmental mixtures.";
RL   Environ. Toxicol. Chem. 29:1367-1376(2010).
//