ID   A549-Dual
AC   CVCL_5I73
DR   cancercelllines; CVCL_5I73
DR   InvivoGen; a549d-nfis
DR   Wikidata; Q54607014
CC   Population: Caucasian.
CC   Characteristics: Stably expresses two reporter genes: SEAP (secreted ALPP) and the Lucia luciferase. In this cell line SEAP is under the control of an IFN-beta minimal promoter fused to 5 NF-kappaB binding sites. The Lucia luciferase reporter gene is under the control of an ISG54 minimal promoter in conjunction with 5 IFN-stimulated response elements (ISRE) (InvivoGen=a549d-nfis).
CC   Sequence variation: Mutation; HGNC; HGNC:6407; KRAS; Simple; p.Gly12Ser (c.34G>A); ClinVar=VCV000012584; Zygosity=Homozygous (from parent cell line).
CC   Sequence variation: Mutation; HGNC; HGNC:11389; STK11; Simple; p.Gln37Ter (c.109C>T); ClinVar=VCV000376334; Zygosity=Homozygous (from parent cell line).
CC   Genetic integration: Method=Transfection; Gene=HGNC; HGNC:439; ALPP.
CC   Genetic integration: Method=Transfection; Gene=UniProtKB; P33967; B.cereus Bsr.
CC   Genetic integration: Method=Transfection; Gene=UniProtKB; P17493; Streptoalloteichus hindustanus ble (BleoR).
CC   Genetic integration: Method=Transfection; Gene=Lucia luciferase (Note=Synthetic gene designed on natural secreted luciferase genes from marine copepods).
CC   Derived from site: In situ; Lung; UBERON=UBERON_0002048.
DI   NCIt; C3512; Lung adenocarcinoma
OX   NCBI_TaxID=9606; ! Homo sapiens (Human)
HI   CVCL_0023 ! A-549
SX   Male
AG   58Y
CA   Cancer cell line
DT   Created: 14-12-15; Last updated: 10-04-25; Version: 15
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