ID   HT29-18-N2
AC   CVCL_5942
SY   HT29-18N2; HT-29-18 N2; N2
DR   cancercelllines; CVCL_5942
DR   Wikidata; Q54896589
RX   PubMed=2154122;
RX   PubMed=3360261;
RX   PubMed=3611191;
RX   PubMed=8589882;
RX   PubMed=12388101;
CC   Population: Caucasian.
CC   Characteristics: Mucus-secreting clone of parent cell line. When grown in the absence of glucose they form homogeneous epithelial monolayers of columnar cells with typical goblet cell morphology (PubMed=3360261).
CC   Sequence variation: Mutation; HGNC; 583; APC; Simple; p.Glu853Ter (c.2557G>T); ClinVar=VCV001071632; Zygosity=Heterozygous (from parent cell line).
CC   Sequence variation: Mutation; HGNC; 583; APC; Simple; p.Thr1556Asnfs*3 (c.4666dupA) (c.4666_4667insA); ClinVar=VCV000428112; Zygosity=Heterozygous (from parent cell line).
CC   Sequence variation: Mutation; HGNC; 1097; BRAF; Simple; p.Val600Glu (c.1799T>A); ClinVar=VCV000013961; Zygosity=Heterozygous (from parent cell line).
CC   Sequence variation: Mutation; HGNC; 8975; PIK3CA; Simple; p.Pro449Thr (c.1345C>A); ClinVar=VCV001333250; Zygosity=Heterozygous (from parent cell line).
CC   Sequence variation: Mutation; HGNC; 6770; SMAD4; Simple; p.Gln311Ter (c.931C>T); ClinVar=VCV000230663; Zygosity=Homozygous (from parent cell line).
CC   Sequence variation: Mutation; HGNC; 11998; TP53; Simple; p.Arg273His (c.818G>A); ClinVar=VCV000012366; Zygosity=Homozygous (from parent cell line).
CC   Derived from site: In situ; Colon; UBERON=UBERON_0001155.
DI   NCIt; C4349; Colon adenocarcinoma
OX   NCBI_TaxID=9606; ! Homo sapiens (Human)
HI   CVCL_5940 ! HT29-18
SX   Female
AG   44Y
CA   Cancer cell line
DT   Created: 04-04-12; Last updated: 05-10-23; Version: 23
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RX   PubMed=2154122; DOI=10.1152/ajpgi.1990.258.1.G96;
RA   Lencer W.I., Reinhart F.D., Neutra M.R.;
RT   "Interaction of cholera toxin with cloned human goblet cells in
RT   monolayer culture.";
RL   Am. J. Physiol. 258:G96-102(1990).
//
RX   PubMed=3360261; DOI=10.1016/0016-5085(88)90678-6;
RA   Phillips T.E., Huet C., Bilbo P.R., Podolsky D.K., Louvard D.,
RA   Neutra M.R.;
RT   "Human intestinal goblet cells in monolayer culture: characterization
RT   of a mucus-secreting subclone derived from the HT29 colon
RT   adenocarcinoma cell line.";
RL   Gastroenterology 94:1390-1403(1988).
//
RX   PubMed=3611191; DOI=10.1083/jcb.105.1.345;
RA   Huet C., Sahuquillo-Merino C., Coudrier E., Louvard D.;
RT   "Absorptive and mucus-secreting subclones isolated from a multipotent
RT   intestinal cell line (HT-29) provide new models for cell polarity and
RT   terminal differentiation.";
RL   J. Cell Biol. 105:345-357(1987).
//
RX   PubMed=8589882; DOI=10.1007/BF02634251;
RA   Phillips T.E., Ramos R., Duncan S.L.;
RT   "HT29-18N2 differentiation in a protein-free medium.";
RL   In Vitro Cell. Dev. Biol. Anim. 31:421-423(1995).
//
RX   PubMed=12388101; DOI=10.1152/ajpcell.00010.2002;
RA   Steinbrecher K.A., Rudolph J.A., Luo G.-J., Cohen M.B.;
RT   "Coordinate upregulation of guanylin and uroguanylin expression by
RT   hypertonicity in HT29-18-N2 cells.";
RL   Am. J. Physiol. Cell Physiol. 283:C1729-C1737(2002).
//