ID   MC3T3-E1 Subclone 4
AC   CVCL_5440
SY   MC3T3, E1 subgroup-4 clone; MC3T3-E1(C4); MC-4
DR   BTO; BTO:0004473
DR   CLO; CLO_0007586
DR   ATCC; CRL-2593
DR   IZSLER; BS CL 181
DR   TOKU-E; 2372
DR   Wikidata; Q54904205
RX   CelloPub=CLPUB00625;
RX   PubMed=10352097;
RX   PubMed=23430347;
CC   Characteristics: Exhibits high levels of osteoblast differentiation after growth in ascorbic acid and 3 to 4 mM inorganic phosphate. Forms a well mineralized extracellular matrix (ECM) after 10 days (ATCC=CRL-2593).
CC   Doubling time: 51.6 hours (CelloPub=CLPUB00625); ~38 hours (ATCC=CRL-2593).
CC   Miscellaneous: STR profile from personal communication of Almeida J.L.; PubMed=23430347 has a different value for STR 6-4 (17) than that submitted (18) due to a change in the marker motif.
CC   Derived from site: In situ; Bone, calvaria; UBERON=UBERON_0004339.
CC   Cell type: Osteoblast; CL=CL_0000062.
CC   Breed/subspecies: C57BL/6.
ST   Source(s): Direct_author_submission; PubMed=23430347
ST   Mouse STR 1-1: 16,17
ST   Mouse STR 1-2: 19
ST   Mouse STR 11-2: 16
ST   Mouse STR 12-1: 17
ST   Mouse STR 13-1: 16
ST   Mouse STR 15-3: 22.3
ST   Mouse STR 17-2: 16
ST   Mouse STR 18-3: 15
ST   Mouse STR 19-2: 13
ST   Mouse STR 2-1: 16
ST   Mouse STR 3-2: 14
ST   Mouse STR 4-2: 20.3
ST   Mouse STR 5-5: 17
ST   Mouse STR 6-4: 18
ST   Mouse STR 6-7: 17
ST   Mouse STR 7-1: 26.2
ST   Mouse STR 8-1: 16
ST   Mouse STR X-1: 28
OX   NCBI_TaxID=10090; ! Mus musculus (Mouse)
HI   CVCL_0409 ! MC3T3-E1
SX   Sex unspecified
AG   <1D
CA   Spontaneously immortalized cell line
DT   Created: 04-04-12; Last updated: 29-06-23; Version: 18
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RX   CelloPub=CLPUB00625;
RA   Lau P., Hellweg C.E., Kirchner S., Arenz A., Baumstark-Khan C.,
RA   Horneck G.;
RT   "Induction of DNA-strand breaks after X-irradiation in murine bone
RT   cells of various differentiation capacities.";
RL   (In) Proceedings of 35th COSPAR Scientific Assembly; pp.1960-1960; Paris (2004).
//
RX   PubMed=10352097; DOI=10.1359/jbmr.1999.14.6.893;
RA   Wang D., Christensen K., Chawla K., Xiao G.-Z., Krebsbach P.H.,
RA   Franceschi R.T.;
RT   "Isolation and characterization of MC3T3-E1 preosteoblast subclones
RT   with distinct in vitro and in vivo differentiation/mineralization
RT   potential.";
RL   J. Bone Miner. Res. 14:893-903(1999).
//
RX   PubMed=23430347; DOI=10.1007/s10616-013-9545-7;
RA   Almeida J.L., Hill C.R., Cole K.D.;
RT   "Mouse cell line authentication.";
RL   Cytotechnology 66:133-147(2014).
//