ID   MEF (DR4)
AC   CVCL_5277
SY   DR4 MEF; DR-4 MEF
DR   CLO; CLO_0002814
DR   ATCC; SCRC-1045
DR   CCRID; 3101MOUSCSP108
DR   Wikidata; Q54905024
RX   PubMed=9278500;
CC   Characteristics: Expresses transgenes that make this cell line resistant to three different drugs: geneticin (G418), puromycin and hygromycin. KO of Hprt1 makes the cell line resistant to 6-tioguanine (PubMed=9278500).
CC   Knockout cell: Method=KO mouse; MGI; MGI:96217; Hprt1.
CC   Genetic integration: Method=Transgenic mouse; Gene=UniProtKB; P00557; Escherichia coli hygromycin-B 4-O-kinase (hph) (HygR).
CC   Genetic integration: Method=Transgenic mouse; Gene=UniProtKB; P13249; S.alboniger pac (PuroR).
CC   Genetic integration: Method=Transgenic mouse; Gene=UniProtKB; P00552; Transposon Tn5 neo.
CC   Derived from site: In situ; Embryo; UBERON=UBERON_0000922.
CC   Cell type: Fibroblast; CL=CL_0000057.
CC   Breed/subspecies: DR4.
OX   NCBI_TaxID=10090; ! Mus musculus (Mouse)
SX   Mixed sex
AG   14FD
CA   Finite cell line
DT   Created: 04-04-12; Last updated: 10-04-25; Version: 15
//
RX   PubMed=9278500; DOI=10.1093/nar/25.18.3745; PMCID=PMC146932;
RA   Tucker K.L., Wang Y.-K., Dausman J., Jaenisch R.;
RT   "A transgenic mouse strain expressing four drug-selectable marker
RT   genes.";
RL   Nucleic Acids Res. 25:3745-3746(1997).
//