ID   HaCaT II-4
AC   CVCL_3868
SY   HaCaT-ras clone II-4; HaCaT-ras II-4; II-4
DR   BTO; BTO_0005111
DR   CLS; 300495
DR   PRIDE; PXD013113
DR   Wikidata; Q54872312
RX   PubMed=11583982;
RX   PubMed=17056607;
RX   PubMed=20097197;
CC   Population: Caucasian.
CC   Characteristics: Tumorigenic.
CC   Sequence variation: Mutation; HGNC; HGNC:11998; TP53; Simple; p.His179Tyr (c.535C>T); ClinVar=VCV000127815; Zygosity=Heterozygous (from parent cell line).
CC   Sequence variation: Mutation; HGNC; HGNC:11998; TP53; Simple; p.Arg282Trp (c.843_844CC>TT); Zygosity=Heterozygous (from parent cell line).
CC   Genetic integration: Method=Transfection/transduction; Gene=HGNC; HGNC:5173; HRAS.
CC   Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; P00552; Transposon Tn5 neo.
CC   Derived from site: In situ; Back, skin, epidermis; UBERON=UBERON_0001068+UBERON_0001003.
CC   Cell type: Keratinocyte; CL=CL_0000312.
ST   Source(s): CLS=300495
ST   Amelogenin: X
ST   CSF1PO: 9,11
ST   D10S1248: 14,15
ST   D12S391: 18,23
ST   D13S317: 10,12
ST   D16S539: 9,12
ST   D18S51: 12
ST   D19S433: 13,14
ST   D1S1656: 11,12
ST   D21S11: 28,30.2
ST   D22S1045: 15,16
ST   D2S1338: 17,25
ST   D2S441: 11
ST   D3S1358: 16
ST   D5S818: 12
ST   D7S820: 9,11
ST   D8S1179: 14
ST   FGA: 24,27.2
ST   SE33: 15,18.2
ST   TH01: 9.3
ST   TPOX: 11,12
ST   vWA: 16,17
OX   NCBI_TaxID=9606; ! Homo sapiens (Human)
HI   CVCL_0038 ! HaCaT
SX   Male
AG   62Y
CA   Spontaneously immortalized cell line
DT   Created: 04-04-12; Last updated: 19-12-24; Version: 19
//
RX   PubMed=11583982; DOI=10.1016/S0002-9440(10)62541-2; PMCID=PMC1850484;
RA   Mueller M.M., Peter W., Mappes M., Huelsen A., Steinbauer H.,
RA   Boukamp P., Vaccariello M.A., Garlick J.A., Fusenig N.E.;
RT   "Tumor progression of skin carcinoma cells in vivo promoted by clonal
RT   selection, mutagenesis, and autocrine growth regulation by granulocyte
RT   colony-stimulating factor and granulocyte-macrophage
RT   colony-stimulating factor.";
RL   Am. J. Pathol. 159:1567-1579(2001).
//
RX   PubMed=17056607; DOI=10.1093/carcin/bgl188;
RA   Willhauck M.J., Mirancea N., Vosseler S., Pavesio A., Boukamp P.,
RA   Mueller M.M., Fusenig N.E., Stark H.-J.;
RT   "Reversion of tumor phenotype in surface transplants of skin SCC cells
RT   by scaffold-induced stroma modulation.";
RL   Carcinogenesis 28:595-610(2007).
//
RX   PubMed=20097197; DOI=10.1016/j.yexcr.2010.01.020;
RA   Rasanen K., Vaheri A.;
RT   "Proliferation and motility of HaCaT keratinocyte derivatives is
RT   enhanced by fibroblast nemosis.";
RL   Exp. Cell Res. 316:1739-1747(2010).
//