ID   HaCaT II-4
AC   CVCL_3868
SY   HaCaT-ras clone II-4; HaCaT-ras II-4; II-4
DR   BTO; BTO_0005111
DR   CLS; 300495
DR   PRIDE; PXD013113
DR   Wikidata; Q54872312
RX   PubMed=11583982;
RX   PubMed=17056607;
RX   PubMed=20097197;
CC   Population: Caucasian.
CC   Characteristics: Tumorigenic.
CC   Sequence variation: Mutation; HGNC; HGNC:11998; TP53; Simple; p.His179Tyr (c.535C>T); ClinVar=VCV000127815; Zygosity=Heterozygous (from parent cell line).
CC   Sequence variation: Mutation; HGNC; HGNC:11998; TP53; Simple; p.Arg282Trp (c.843_844CC>TT); Zygosity=Heterozygous (from parent cell line).
CC   Genetic integration: Method=Transfection; Gene=HGNC; HGNC:5173; HRAS.
CC   Genetic integration: Method=Transfection; Gene=UniProtKB; P00552; Klebsiella pneumoniae transposon Tn5 neo.
CC   Derived from site: In situ; Back, skin, epidermis; UBERON=UBERON_0001068+UBERON_0001003.
CC   Cell type: Keratinocyte; CL=CL_0000312.
ST   Source(s): CLS=300495
ST   Amelogenin: X
ST   CSF1PO: 9,11
ST   D10S1248: 14,15
ST   D12S391: 18,23
ST   D13S317: 10,12
ST   D16S539: 9,12
ST   D18S51: 12
ST   D19S433: 13,14
ST   D1S1656: 11,12
ST   D21S11: 28,30.2
ST   D22S1045: 15,16
ST   D2S1338: 17,25
ST   D2S441: 11
ST   D3S1358: 16
ST   D5S818: 12
ST   D7S820: 9,11
ST   D8S1179: 14
ST   FGA: 24,27.2
ST   SE33: 15,18.2
ST   TH01: 9.3
ST   TPOX: 11,12
ST   vWA: 16,17
OX   NCBI_TaxID=9606; ! Homo sapiens (Human)
HI   CVCL_0038 ! HaCaT
SX   Male
AG   62Y
CA   Spontaneously immortalized cell line
DT   Created: 04-04-12; Last updated: 27-11-25; Version: 21
//
RX   PubMed=17056607; DOI=10.1093/carcin/bgl188;
RA   Willhauck, Michael J.
RA   Mirancea, Nicolae
RA   Vosseler, Silvia
RA   Pavesio, Alessandra
RA   Boukamp, Petra
RA   Mueller, Margareta M.
RA   Fusenig, Norbert E.
RA   Stark, Hans-Jurgen
RT   "Reversion of tumor phenotype in surface transplants of skin SCC cells
RT   by scaffold-induced stroma modulation.";
RL   Carcinogenesis 28:595-610(2007).
//
RX   PubMed=20097197; DOI=10.1016/j.yexcr.2010.01.020;
RA   Rasanen, Kati
RA   Vaheri, Antti
RT   "Proliferation and motility of HaCaT keratinocyte derivatives is
RT   enhanced by fibroblast nemosis.";
RL   Exp. Cell Res. 316:1739-1747(2010).
//
RX   PubMed=11583982; DOI=10.1016/S0002-9440(10)62541-2; PMCID=PMC1850484;
RA   Mueller, Margareta M.
RA   Peter, Wolfgang
RA   Mappes, Marion
RA   Huelsen, Andrea
RA   Steinbauer, Heinrich
RA   Boukamp, Petra
RA   Vaccariello, Michael Anthony
RA   Garlick, Jonathan A.
RA   Fusenig, Norbert E.
RT   "Tumor progression of skin carcinoma cells in vivo promoted by clonal
RT   selection, mutagenesis, and autocrine growth regulation by granulocyte
RT   colony-stimulating factor and granulocyte-macrophage
RT   colony-stimulating factor.";
RL   Am. J. Pathol. 159:1567-1579(2001).
//