ID   RKO-E6
AC   CVCL_3787
SY   RKO E6; RKOE6; RC10-1
DR   BTO; BTO_0001891
DR   CLO; CLO_0008827
DR   EFO; EFO_0002318
DR   ArrayExpress; E-MTAB-38
DR   ArrayExpress; E-MTAB-2706
DR   ATCC; CRL-2578
DR   BioSample; SAMN03471683
DR   BioSample; SAMN03472970
DR   cancercelllines; CVCL_3787
DR   CCRID; 3101HUMTCHu118
DR   Cell_Model_Passport; SIDM01253
DR   CLS; 305135
DR   ColonAtlas; RKOE6
DR   Cosmic; 1184102
DR   Cosmic; 1187317
DR   EGA; EGAS00001000610
DR   IGRhCellID; RKOE6
DR   PharmacoDB; RKOE6_1317_2019
DR   Progenetix; CVCL_3787
DR   Wikidata; Q54950558
RX   PubMed=7700629;
RX   PubMed=8202487;
RX   PubMed=8387205;
RX   PubMed=15161374;
RX   PubMed=20215515;
RX   PubMed=25485619;
RX   PubMed=25877200;
RX   PubMed=26589293;
CC   HLA typing: A*03:01,03:01; B*18:01,37:04; C*07:01,07:01 (PubMed=26589293).
CC   Sequence variation: Mutation; HGNC; HGNC:173; ACVR2A; Simple; p.Lys437Argfs*5 (c.1310delA); dbSNP=rs764719749; Zygosity=Heterozygous (from parent cell line).
CC   Sequence variation: Mutation; HGNC; HGNC:1097; BRAF; Simple; p.Val600Glu (c.1799T>A); ClinVar=VCV000013961; Zygosity=Heterozygous (from parent cell line).
CC   Sequence variation: Mutation; HGNC; HGNC:8975; PIK3CA; Simple; p.His1047Arg (c.3140A>G); ClinVar=VCV000013652; Zygosity=Heterozygous (from parent cell line).
CC   Sequence variation: Mutation; HGNC; HGNC:11773; TGFBR2; Simple; p.Lys128Serfs*35 (c.383delA); ClinVar=VCV000477546; Zygosity=Heterozygous (from parent cell line).
CC   Sequence variation: Mutation; HGNC; HGNC:11773; TGFBR2; Simple; p.Leu452Pro (c.1355T>C); ClinVar=VCV000930524; Zygosity=Heterozygous (from parent cell line).
CC   Genetic integration: Method=Transfection; Gene=UniProtKB; P03126; Human papillomavirus type 16 protein E6.
CC   Genetic integration: Method=Transfection; Gene=UniProtKB; P00552; Klebsiella pneumoniae transposon Tn5 neo.
CC   Omics: Transcriptomics; RNAseq.
CC   Omics: Variations; SNP array analysis.
CC   Caution: We believe that the clone called RKO-E6 by PubMed=7700629 is the clone RC10-1 originally described by PubMed=8387205 but it is also possible that it is RKO RC10-2 (Cellosaurus=CVCL_E7ET) or RKO RC10-3 (Cellosaurus=CVCL_E7EU) or RKO RC10-4 (Cellosaurus=CVCL_E7EV).
CC   Derived from site: In situ; Colon; UBERON=UBERON_0001155.
ST   Source(s): ATCC=CRL-2578; PubMed=25877200
ST   Amelogenin: X
ST   CSF1PO: 8,11
ST   D13S317: 8,12
ST   D16S539: 13,13.1
ST   D18S51: 11,13
ST   D21S11: 28,30
ST   D3S1358: 16,19
ST   D5S818: 12,13
ST   D7S820: 8,10
ST   D8S1179: 9,13,14
ST   FGA: 19,22
ST   Penta D: 10,11
ST   Penta E: 11,13
ST   TH01: 6,10 (PubMed=25877200)
ST   TH01: 6.2,10 (ATCC=CRL-2578)
ST   TPOX: 10,11
ST   vWA: 16
DI   NCIt; C4910; Colon carcinoma
OX   NCBI_TaxID=9606; ! Homo sapiens (Human)
HI   CVCL_0504 ! RKO
SX   Sex unspecified
AG   Age unspecified
CA   Cancer cell line
DT   Created: 04-04-12; Last updated: 14-08-25; Version: 30
//
RX   PubMed=15161374; DOI=10.1667/rr3160;
RA   Franken, Nicolaas A.P.
RA   van Bree, Chris
RA   Haveman, Jaap
RT   "Differential response to radiation of TP53-inactivated cells by
RT   overexpression of dominant-negative mutant TP53 or HPVE6.";
RL   Radiat. Res. 161:504-510(2004).
//
RX   PubMed=25485619; DOI=10.1038/nbt.3080;
RA   Klijn, Christiaan
RA   Durinck, Steffen
RA   Stawiski, Eric W.
RA   Haverty, Peter M.
RA   Jiang, Zhao-Shi
RA   Liu, Han-Bin
RA   Degenhardt, Jeremiah
RA   Mayba, Oleg
RA   Gnad, Florian
RA   Liu, Jin-Feng
RA   Pau, Gregoire
RA   Reeder, Jens
RA   Cao, Yi
RA   Mukhyala, Kiran
RA   Selvaraj, Suresh K.
RA   Yu, Ma-Mie
RA   Zynda, Gregory J.
RA   Brauer, Matthew J.
RA   Wu, Thomas D.
RA   Gentleman, Robert Clifford
RA   Manning, Gerard
RA   Yauch, Robert L.
RA   Bourgon, Richard
RA   Stokoe, David
RA   Modrusan, Zora
RA   Neve, Richard M.
RA   de Sauvage, Frederic J.
RA   Settleman, Jeffrey
RA   Seshagiri, Somasekar
RA   Zhang, Ze-Min
RT   "A comprehensive transcriptional portrait of human cancer cell
RT   lines.";
RL   Nat. Biotechnol. 33:306-312(2015).
//
RX   PubMed=26589293; DOI=10.1186/s13073-015-0240-5; PMCID=PMC4653878;
RA   Scholtalbers, Jelle
RA   Boegel, Sebastian
RA   Bukur, Thomas
RA   Byl, Marius
RA   Goerges, Sebastian
RA   Sorn, Patrick
RA   Loewer, Martin
RA   Sahin, Ugur
RA   Castle, John C.
RT   "TCLP: an online cancer cell line catalogue integrating HLA type,
RT   predicted neo-epitopes, virus and gene expression.";
RL   Genome Med. 7:118.1-118.7(2015).
//
RX   PubMed=20215515; DOI=10.1158/0008-5472.CAN-09-3458; PMCID=PMC2881662;
RA   Rothenberg, S. Michael
RA   Mohapatra, Gayatry
RA   Rivera, Miguel N.
RA   Winokur, Daniel
RA   Greninger, Patricia
RA   Nitta, Mai
RA   Sadow, Peter M.
RA   Sooriyakumar, Gaya
RA   Brannigan, Brian W.
RA   Ulman, Matthew J.
RA   Perera, Rushika M.
RA   Wang, Rui
RA   Tam, Angela
RA   Ma, Xiao-Jun
RA   Erlander, Mark
RA   Sgroi, Dennis C.
RA   Rocco, James W.
RA   Lingen, Mark W.
RA   Cohen, Ezra E.W.
RA   Louis, David Neil
RA   Settleman, Jeffrey
RA   Haber, Daniel Arie
RT   "A genome-wide screen for microdeletions reveals disruption of
RT   polarity complex genes in diverse human cancers.";
RL   Cancer Res. 70:2158-2164(2010).
//
RX   PubMed=7700629;
RA   Smith, Martin L.
RA   Chen, I-Tsuen
RA   Zhan, Qi-Min
RA   O'Connor, Patrick M.
RA   Fornace, Albert J. Jr.
RT   "Involvement of the p53 tumor suppressor in repair of u.v.-type DNA
RT   damage.";
RL   Oncogene 10:1053-1059(1995).
//
RX   PubMed=25877200; DOI=10.1038/nature14397;
RA   Yu, Ma-Mie
RA   Selvaraj, Suresh K.
RA   Liang-Chu, May M.Y.
RA   Aghajani, Sahar
RA   Busse, Matthew
RA   Yuan, Jean
RA   Lee, Genee
RA   Peale, Franklin V.
RA   Klijn, Christiaan
RA   Bourgon, Richard
RA   Kaminker, Joshua S.
RA   Neve, Richard M.
RT   "A resource for cell line authentication, annotation and quality
RT   control.";
RL   Nature 520:307-311(2015).
//
RX   PubMed=8202487; DOI=10.1073/pnas.91.12.5320; PMCID=PMC43986;
RA   Slebos, Robbert J.C.
RA   Lee, Mann H.
RA   Plunkett, Beverly S.
RA   Kessis, Theodore D.
RA   Williams, Bart O.
RA   Jacks, Tyler
RA   Hedrick Ellenson, Lora
RA   Kastan, Michael Barry
RA   Cho, Kathleen R.
RT   "p53-dependent G1 arrest involves pRB-related proteins and is
RT   disrupted by the human papillomavirus 16 E7 oncoprotein.";
RL   Proc. Natl. Acad. Sci. U.S.A. 91:5320-5324(1994).
//
RX   PubMed=8387205; DOI=10.1073/pnas.90.9.3988; PMCID=PMC46431;
RA   Kessis, Theodore D.
RA   Slebos, Robbert J.C.
RA   Nelson, William George
RA   Kastan, Michael Barry
RA   Plunkett, Beverly S.
RA   Han, Sung M.
RA   Lorincz, Attila T.
RA   Hedrick Ellenson, Lora
RA   Cho, Kathleen R.
RT   "Human papillomavirus 16 E6 expression disrupts the p53-mediated
RT   cellular response to DNA damage.";
RL   Proc. Natl. Acad. Sci. U.S.A. 90:3988-3992(1993).
//