ID   J558
AC   CVCL_3529
DR   CLO; CLO_0007001
DR   ATCC; TIB-6
DR   BioSample; SAMN11397662
DR   Wikidata; Q28243888
RX   PubMed=5483159;
RX   PubMed=6157734;
RX   PubMed=31220119;
CC   Problematic cell line: Potentially contaminated. The stock distributed by ATCC (TIB-6) has a STR profile that matches at 95% with that of BALB/3T3 clone A31 (Cellosaurus=CVCL_0184). As this is the parent cell line to a cell line J558L (Cellosaurus=CVCL_3949) which is used a fusion cell line for the production of hybridomas ICLAC is currently investigating if authentic stocks of J558 still exist.
CC   Part of: Tumor Immunology Bank (TIB) collection from Salk (transferred to ATCC in 1981).
CC   Monoclonal antibody isotype: IgA, lambda.
CC   Monoclonal antibody target: ChEBI; CHEBI_52071; Dextran.
CC   Discontinued: BioSample; SAMN11397662; true.
CC   Cell type: B-cell; CL=CL_0000236.
CC   Breed/subspecies: BALB/c.
ST   Source(s): PubMed=31220119
ST   Mouse STR 1-1: 16
ST   Mouse STR 1-2: 17
ST   Mouse STR 2-1: 16
ST   Mouse STR 3-2: 14
ST   Mouse STR 4-2: 21.3
ST   Mouse STR 5-5: 14
ST   Mouse STR 6-4: 18
ST   Mouse STR 6-7: 12
ST   Mouse STR 7-1: 25.2
ST   Mouse STR 8-1: 13
ST   Mouse STR 11-2: 17
ST   Mouse STR 12-1: 16
ST   Mouse STR 13-1: 16.2
ST   Mouse STR 15-3: 22.3
ST   Mouse STR 17-2: 16
ST   Mouse STR 18-3: 18
ST   Mouse STR 19-2: 14
ST   Mouse STR X-1: 25
DI   NCIt; C125417; Mouse multiple myeloma
OX   NCBI_TaxID=10090; ! Mus musculus (Mouse)
CA   Cancer cell line
DT   Created: 04-04-12; Last updated: 10-04-25; Version: 16
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RX   PubMed=5483159; DOI=10.1038/2281045a0;
RA   Weigert M.G., Cesari I.M., Yonkovich S.J., Cohn M.;
RT   "Variability in the lambda light chain sequences of mouse antibody.";
RL   Nature 228:1045-1047(1970).
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RX   PubMed=6157734; DOI=10.4049/jimmunol.125.4.1454;
RA   Bhoopalam N., Heller P., Meyerstein N., Hall L.;
RT   "Effect of dextran-S (alpha, 1-3 dextran) on the growth of
RT   plasmacytomas MOPC-104E and J558.";
RL   J. Immunol. 125:1454-1458(1980).
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RX   PubMed=31220119; DOI=10.1371/journal.pone.0218412; PMCID=PMC6586308;
RA   Almeida J.L., Dakic A., Kindig K., Kone M., Letham D.L.D., Langdon S.,
RA   Peat R., Holding-Pillai J., Hall E.M., Ladd M., Shaffer M.D., Berg H.,
RA   Li J.-L., Wigger G., Lund S., Steffen C.R., Fransway B.B., Geraghty B.,
RA   Natoli M., Bauer B.A., Gollin S.M., Lewis D.W., Reid Y.A.;
RT   "Interlaboratory study to validate a STR profiling method for
RT   intraspecies identification of mouse cell lines.";
RL   PLoS ONE 14:e0218412.1-e0218412.24(2019).
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