ID   J558
AC   CVCL_3529
DR   CLO; CLO_0007001
DR   ATCC; TIB-6
DR   BioSample; SAMN11397662
DR   Wikidata; Q28243888
RX   PubMed=5483159;
RX   PubMed=6157734;
RX   PubMed=31220119;
CC   Problematic cell line: Potentially contaminated. The stock distributed by ATCC (TIB-6) has a STR profile that matches at 95% with that of BALB/3T3 clone A31 (Cellosaurus=CVCL_0184). As this is the parent cell line to a cell line J558L (Cellosaurus=CVCL_3949) which is used a fusion cell line for the production of hybridomas ICLAC is currently investigating if authentic stocks of J558 still exist.
CC   Part of: Tumor Immunology Bank (TIB) collection from Salk (transferred to ATCC in 1981).
CC   Monoclonal antibody isotype: IgA, lambda.
CC   Monoclonal antibody target: ChEBI; CHEBI_52071; Dextran.
CC   Discontinued: BioSample; SAMN11397662; true.
CC   Cell type: Plasma cell; CL=CL_0000786.
CC   Breed/subspecies: BALB/c.
ST   Source(s): PubMed=31220119
ST   Mouse STR 1-1: 16
ST   Mouse STR 1-2: 17
ST   Mouse STR 2-1: 16
ST   Mouse STR 3-2: 14
ST   Mouse STR 4-2: 21.3
ST   Mouse STR 5-5: 14
ST   Mouse STR 6-4: 18
ST   Mouse STR 6-7: 12
ST   Mouse STR 7-1: 25.2
ST   Mouse STR 8-1: 13
ST   Mouse STR 11-2: 17
ST   Mouse STR 12-1: 16
ST   Mouse STR 13-1: 16.2
ST   Mouse STR 15-3: 22.3
ST   Mouse STR 17-2: 16
ST   Mouse STR 18-3: 18
ST   Mouse STR 19-2: 14
ST   Mouse STR X-1: 25
DI   NCIt; C125417; Mouse multiple myeloma
OX   NCBI_TaxID=10090; ! Mus musculus (Mouse)
SX   Sex unspecified
AG   Age unspecified
CA   Cancer cell line
DT   Created: 04-04-12; Last updated: 14-08-25; Version: 17
CH   CVCL_E3HM ! HyCyte J558-Luc
CH   CVCL_3949 ! J558L
//
RX   PubMed=6157734; DOI=10.4049/jimmunol.125.4.1454;
RA   Bhoopalam, Nirmala
RA   Heller, Paul
RA   Meyerstein, Naomi
RA   Hall, Lemuel
RT   "Effect of dextran-S (alpha, 1-3 dextran) on the growth of
RT   plasmacytomas MOPC-104E and J558.";
RL   J. Immunol. 125:1454-1458(1980).
//
RX   PubMed=5483159; DOI=10.1038/2281045a0;
RA   Weigert, Martin G.
RA   Cesari, Italo M.
RA   Yonkovich, Shirlee J.
RA   Cohn, Melvin
RT   "Variability in the lambda light chain sequences of mouse antibody.";
RL   Nature 228:1045-1047(1970).
//
RX   PubMed=31220119; DOI=10.1371/journal.pone.0218412; PMCID=PMC6586308;
RA   Almeida, Jamie L.
RA   Dakic, Aleksandra
RA   Kindig, Karin
RA   Kone, Maikan
RA   Letham, Deborah Lee Dormady
RA   Langdon, Scott
RA   Peat, Ruth
RA   Holding-Pillai, Jayamalini
RA   Hall, Erin M.
RA   Ladd, Mark
RA   Shaffer, Megan D.
RA   Berg, Heath
RA   Li, Jin-Liang
RA   Wigger, Georges
RA   Lund, Steve
RA   Steffen, Carolyn R.
RA   Fransway, Barbara B.
RA   Geraghty, Bob
RA   Natoli, Manuela
RA   Bauer, Beth A.
RA   Gollin, Susanne M.
RA   Lewis, Dale W.
RA   Reid, Yvonne A.
RT   "Interlaboratory study to validate a STR profiling method for
RT   intraspecies identification of mouse cell lines.";
RL   PLoS ONE 14:e0218412.1-e0218412.24(2019).
//