ID   KNS-89
AC   CVCL_2800
SY   KN-S89; KNS89
DR   BioSample; SAMN03151976
DR   cancercelllines; CVCL_2800
DR   CGH-DB; 147-1
DR   Cosmic; 687576
DR   IARC_TP53; 21438
DR   JCRB; IFO50360
DR   Wikidata; Q54900255
RX   CelloPub=CLPUB00152;
RX   PubMed=16232199;
RX   PubMed=20143388;
RX   PubMed=22570425;
WW   Info; ICLAC; -; https://iclac.org/wp-content/uploads/Cross-Contaminations_v13_distribution.xlsx
WW   Info; JCRB; -; https://cellbank.nibiohn.go.jp/legacy/cellbank/qualitycontrol/identification/summary.htm
CC   Problematic cell line: Contaminated. Shown to be a U-251MG derivative (PubMed=20143388; PubMed=22570425). Originally thought to originate from a 66 year old male patient with a gliosarcoma.
CC   Registration: International Cell Line Authentication Committee, Register of Misidentified Cell Lines; ICLAC-00323.
CC   Population: Caucasian.
CC   Doubling time: 42 hours (CelloPub=CLPUB00152).
CC   Sequence variation: Mutation; HGNC; HGNC:9588; PTEN; Simple; p.Glu242Valfs*15 (c.723_724dupTG); ClinVar=VCV000536553; Zygosity=Unspecified (from parent cell line).
CC   Sequence variation: Mutation; HGNC; HGNC:11730; TERT; Simple; c.1-124C>T (c.228C>T) (C228T); Zygosity=Unspecified; Note=In promoter (from parent cell line).
CC   Sequence variation: Mutation; HGNC; HGNC:11998; TP53; Simple; p.Arg273His (c.818G>A); ClinVar=VCV000012366; Zygosity=Unspecified (from parent cell line).
CC   Discontinued: JCRB; IFO50360; true.
CC   Derived from site: In situ; Brain, parietal lobe; UBERON=UBERON_0001872.
ST   Source(s): JCRB=IFO50360
ST   Amelogenin: X
ST   CSF1PO: 11,12
ST   D13S317: 10,11
ST   D16S539: 12
ST   D5S818: 11,12
ST   D7S820: 10,12
ST   TH01: 9.3
ST   TPOX: 8
ST   vWA: 16,18
DI   NCIt; C60781; Astrocytoma
DI   ORDO; Orphanet_94; Astrocytoma
OX   NCBI_TaxID=9606; ! Homo sapiens (Human)
HI   CVCL_0021 ! U-251MG
SX   Male
AG   75Y
CA   Cancer cell line
DT   Created: 04-04-12; Last updated: 10-04-25; Version: 31
//
RX   PubMed=16232199; DOI=10.1111/j.1349-7006.2005.00099.x; PMCID=PMC11159392;
RA   Saigusa, Kuniyasu
RA   Hashimoto, Naoya
RA   Tsuda, Hitoshi
RA   Yokoi, Sana
RA   Maruno, Motohiko
RA   Yoshimine, Toshiki
RA   Aoyagi, Masaru
RA   Ohno, Kikuo
RA   Imoto, Issei
RA   Inazawa, Johji
RT   "Overexpressed Skp2 within 5p amplification detected by array-based
RT   comparative genomic hybridization is associated with poor prognosis of
RT   glioblastomas.";
RL   Cancer Sci. 96:676-683(2005).
//
RX   PubMed=20143388; DOI=10.1002/ijc.25242;
RA   Capes-Davis, Amanda
RA   Theodosopoulos, George
RA   Atkin, Isobel
RA   Drexler, Hans Gunther
RA   Kohara, Arihiro
RA   MacLeod, Roderick A.F.
RA   Masters, John R.W.
RA   Nakamura, Yukio
RA   Reid, Yvonne A.
RA   Reddel, Roger Robert
RA   Freshney, Robert Ian
RT   "Check your cultures! A list of cross-contaminated or misidentified
RT   cell lines.";
RL   Int. J. Cancer 127:1-8(2010).
//
RX   CelloPub=CLPUB00152;
RA   Takeshita, Iwao
RA   Takaki, Tosuke
RA   Nakamura, Takaharu
RA   Maeyama, Ryuutaro
RA   Fukui, Masashi
RA   Kitamura, Katsutoshi
RT   "Established cell lines derived from human gliomas.";
RL   Hum. Cell 3:255-256(1990).
//
RX   PubMed=22570425; DOI=10.1093/neuonc/nos072; PMCID=PMC3367844;
RA   Bady, Pierre
RA   Diserens, Annie-Claire
RA   Castella, Vincent
RA   Kalt, Stefanie
RA   Heinimann, Karl
RA   Hamou, Marie-France
RA   Delorenzi, Mauro
RA   Hegi, Monika E.
RT   "DNA fingerprinting of glioma cell lines and considerations on
RT   similarity measurements.";
RL   Neuro-oncol. 14:701-711(2012).
//