ID   HCT 8
AC   CVCL_2478
SY   HCT-8; HCT8
DR   BTO; BTO:0002255
DR   CLO; CLO_0003667
DR   EFO; EFO_0002189
DR   CLDB; cl1577
DR   CLDB; cl1578
DR   CLDB; cl4949
DR   CLDB; cl5197
DR   AddexBio; C0009017/4907
DR   ArrayExpress; E-MTAB-38
DR   ArrayExpress; E-MTAB-2706
DR   BCRC; 60303
DR   BioSample; SAMN03473138
DR   cancercelllines; CVCL_2478
DR   CCRID; 1101HUM-PUMC000104
DR   CCRID; 3101HUMTCHu18
DR   CCRID; 4201HUM-CCTCC00201
DR   CCTCC; GDC0201
DR   Cell_Model_Passport; SIDM00786
DR   ChEMBL-Cells; CHEMBL3308035
DR   ChEMBL-Targets; CHEMBL613510
DR   CLS; 300210
DR   ColonAtlas; HCT8
DR   Cosmic; 887226
DR   Cosmic; 948853
DR   Cosmic; 983738
DR   Cosmic; 988873
DR   Cosmic; 1043814
DR   Cosmic; 1310946
DR   Cosmic; 1479593
DR   Cosmic; 1609484
DR   Cosmic; 1676735
DR   Cosmic; 2267316
DR   Cosmic; 2301980
DR   Cosmic; 2651867
DR   DepMap; ACH-001084
DR   ECACC; 90032006
DR   EGA; EGAS00001000610
DR   GEO; GSM206504
DR   GEO; GSM274709
DR   GEO; GSM274710
DR   GEO; GSM274723
DR   GEO; GSM513910
DR   GEO; GSM514294
DR   GEO; GSM827344
DR   GEO; GSM1346876
DR   GEO; GSM1374523
DR   GEO; GSM1374524
DR   GEO; GSM1448162
DR   ICLC; HTL99005
DR   IGRhCellID; HCT8
DR   IZSLER; BS TCL 97
DR   KCB; KCB 2011103YJ
DR   KCLB; 10244
DR   LINCS_LDP; LCL-1198
DR   MetaboLights; MTBLS227
DR   PharmacoDB; HCT8_519_2019
DR   Progenetix; CVCL_2478
DR   PubChem_Cell_line; CVCL_2478
DR   Wikidata; Q54882008
RX   PubMed=77569;
RX   PubMed=8422623;
RX   PubMed=9809040;
RX   PubMed=10674020;
RX   PubMed=20215515;
RX   PubMed=20570890;
RX   PubMed=24755471;
RX   PubMed=25485619;
RX   PubMed=25877200;
RX   PubMed=25926053;
RX   PubMed=25944804;
RX   PubMed=26537799;
RX   PubMed=26589293;
RX   PubMed=28196595;
RX   PubMed=31068700;
RX   PubMed=33389257;
WW   https://tcpaportal.org/mclp/
CC   Part of: Cancer Dependency Map project (DepMap) (includes Cancer Cell Line Encyclopedia - CCLE).
CC   Part of: MD Anderson Cell Lines Project.
CC   Population: Caucasian.
CC   Virology: Not susceptible to infection by SARS coronavirus 2 (SARS-CoV-2) (COVID-19) (PubMed=33389257).
CC   Doubling time: 27.49 hours (PubMed=25944804).
CC   HLA typing: A*03,09; B*08 (PubMed=77569).
CC   HLA typing: A*02:01,24:02; B*08:01,35:01; C*04:01,07:01 (PubMed=26589293).
CC   Microsatellite instability: Instable (MSI) (PubMed=10674020; PubMed=24755471; PubMed=25926053).
CC   Sequence variation: Mutation; HGNC; 583; APC; Simple; p.Arg727Met (c.2180G>T); Zygosity=Unspecified (PubMed=24755471).
CC   Sequence variation: Mutation; HGNC; 583; APC; Simple; p.Lys993Asn (c.2979G>T); Zygosity=Unspecified (PubMed=24755471).
CC   Sequence variation: Mutation; HGNC; 583; APC; Simple; p.Ile1417Leufs*2 (c.4248delC); Zygosity=Unspecified (PubMed=24755471).
CC   Sequence variation: Mutation; HGNC; 583; APC; Simple; p.Arg2166Ter (c.6496C>T); ClinVar=VCV000438883; Zygosity=Unspecified (PubMed=24755471).
CC   Sequence variation: Mutation; HGNC; 2514; CTNNB1; Simple; p.Val79Ile (c.235G>A); Zygosity=Unspecified (PubMed=24755471).
CC   Sequence variation: Mutation; HGNC; 6407; KRAS; Simple; p.Gly13Asp (c.38G>A); ClinVar=VCV000012580; Zygosity=Unspecified (PubMed=20570890; PubMed=24755471).
CC   Sequence variation: Mutation; HGNC; 8975; PIK3CA; Simple; p.Glu545Lys (c.1633G>A); ClinVar=VCV000013655; Zygosity=Unspecified (PubMed=20570890; PubMed=24755471).
CC   Sequence variation: Mutation; HGNC; 8975; PIK3CA; Simple; p.Asp549Asn (c.1645G>A); ClinVar=VCV000375900; Zygosity=Unspecified (PubMed=20570890; PubMed=24755471).
CC   Sequence variation: Mutation; HGNC; 6768; SMAD2; Simple; p.Pro164fs*8 (c.490_491insA); Zygosity=Unspecified (PubMed=24755471).
CC   Sequence variation: Mutation; HGNC; 6769; SMAD3; Simple; p.Val244Ile (c.730G>A); ClinVar=VCV000919044; Zygosity=Unspecified (PubMed=24755471).
CC   Omics: N-glycan profiling.
CC   Omics: Protein expression by reverse-phase protein arrays.
CC   Omics: SNP array analysis.
CC   Omics: Transcriptome analysis by microarray.
CC   Omics: Transcriptome analysis by RNAseq.
CC   Misspelling: HCT 18; Note=Occasionally.
ST   Source(s): AddexBio; CCRID; CLS; KCLB; PubMed=25877200; PubMed=25926053
ST   Amelogenin: X (CCRID; PubMed=25877200)
ST   Amelogenin: X,Y (AddexBio; CLS; KCLB; PubMed=25926053)
ST   CSF1PO: 12
ST   D13S317: 8,11
ST   D16S539: 12,13
ST   D18S51: 11,17
ST   D19S433: 14,16
ST   D21S11: 29,32.2
ST   D2S1338: 17,25
ST   D3S1358: 17
ST   D5S818: 13
ST   D7S820: 10,11.3 (CCRID; PubMed=25877200)
ST   D7S820: 10,11.3,12 (AddexBio)
ST   D7S820: 10,12 (CLS; KCLB; PubMed=25926053)
ST   D8S1179: 15
ST   FGA: 22
ST   Penta D: 9,14
ST   Penta E: 7,14
ST   TH01: 7,9.3
ST   TPOX: 8,11
ST   vWA: 18,19
DI   NCIt; C4349; Colon adenocarcinoma
OX   NCBI_TaxID=9606; ! Homo sapiens (Human)
HI   CVCL_3449 ! __Parent_cell_line_of_DLD-1/HCT 8/HCT 15/HRT-18
SX   Male
AG   67Y
CA   Cancer cell line
DT   Created: 04-04-12; Last updated: 05-10-23; Version: 38
//
RX   PubMed=77569; DOI=10.1111/j.1399-0039.1978.tb01259.x;
RA   Espmark J.A., Ahlqvist-Roth L., Sarne L., Persson A.;
RT   "Tissue typing of cells in culture. III. HLA antigens of established
RT   human cell lines. Attempts at typing by the mixed hemadsorption
RT   technique.";
RL   Tissue Antigens 11:279-286(1978).
//
RX   PubMed=8422623; DOI=10.1002/1097-0142(19930115)71:2<315::AID-CNCR2820710208>3.0.CO;2-B;
RA   Tibbetts L.M., Doremus C.M., Tzanakakis G.N., Vezeridis M.P.;
RT   "Liver metastases with 10 human colon carcinoma cell lines in nude
RT   mice and association with carcinoembryonic antigen production.";
RL   Cancer 71:315-321(1993).
//
RX   PubMed=9809040; DOI=10.1016/S0165-4608(98)00081-8;
RA   Vermeulen S.J., Chen T.-R., Speleman F., Nollet F., Van Roy F.M.,
RA   Mareel M.M.;
RT   "Did the four human cancer cell lines DLD-1, HCT-15, HCT-8, and HRT-18
RT   originate from one and the same patient?";
RL   Cancer Genet. Cytogenet. 107:76-79(1998).
//
RX   PubMed=10674020; DOI=10.1016/S0959-8049(99)00206-3;
RA   Ku J.-L., Yoon K.-A., Kim D.-Y., Park J.-G.;
RT   "Mutations in hMSH6 alone are not sufficient to cause the
RT   microsatellite instability in colorectal cancer cell lines.";
RL   Eur. J. Cancer 35:1724-1729(1999).
//
RX   PubMed=20215515; DOI=10.1158/0008-5472.CAN-09-3458;
RA   Rothenberg S.M., Mohapatra G., Rivera M.N., Winokur D., Greninger P.,
RA   Nitta M., Sadow P.M., Sooriyakumar G., Brannigan B.W., Ulman M.J.,
RA   Perera R.M., Wang R., Tam A., Ma X.-J., Erlander M., Sgroi D.C.,
RA   Rocco J.W., Lingen M.W., Cohen E.E.W., Louis D.N., Settleman J.,
RA   Haber D.A.;
RT   "A genome-wide screen for microdeletions reveals disruption of
RT   polarity complex genes in diverse human cancers.";
RL   Cancer Res. 70:2158-2164(2010).
//
RX   PubMed=20570890; DOI=10.1158/0008-5472.CAN-10-0192;
RA   Janakiraman M., Vakiani E., Zeng Z.-S., Pratilas C.A., Taylor B.S.,
RA   Chitale D., Halilovic E., Wilson M., Huberman K., Ricarte Filho J.C.M.,
RA   Persaud Y., Levine D.A., Fagin J.A., Jhanwar S.C., Mariadason J.M.,
RA   Lash A., Ladanyi M., Saltz L.B., Heguy A., Paty P.B., Solit D.B.;
RT   "Genomic and biological characterization of exon 4 KRAS mutations in
RT   human cancer.";
RL   Cancer Res. 70:5901-5911(2010).
//
RX   PubMed=24755471; DOI=10.1158/0008-5472.CAN-14-0013;
RA   Mouradov D., Sloggett C., Jorissen R.N., Love C.G., Li S.,
RA   Burgess A.W., Arango D., Strausberg R.L., Buchanan D., Wormald S.,
RA   O'Connor L., Wilding J.L., Bicknell D.C., Tomlinson I.P.M., Bodmer W.F.,
RA   Mariadason J.M., Sieber O.M.;
RT   "Colorectal cancer cell lines are representative models of the main
RT   molecular subtypes of primary cancer.";
RL   Cancer Res. 74:3238-3247(2014).
//
RX   PubMed=25485619; DOI=10.1038/nbt.3080;
RA   Klijn C., Durinck S., Stawiski E.W., Haverty P.M., Jiang Z.-S.,
RA   Liu H.-B., Degenhardt J., Mayba O., Gnad F., Liu J.-F., Pau G.,
RA   Reeder J., Cao Y., Mukhyala K., Selvaraj S.K., Yu M.-M., Zynda G.J.,
RA   Brauer M.J., Wu T.D., Gentleman R.C., Manning G., Yauch R.L.,
RA   Bourgon R., Stokoe D., Modrusan Z., Neve R.M., de Sauvage F.J.,
RA   Settleman J., Seshagiri S., Zhang Z.-M.;
RT   "A comprehensive transcriptional portrait of human cancer cell
RT   lines.";
RL   Nat. Biotechnol. 33:306-312(2015).
//
RX   PubMed=25877200; DOI=10.1038/nature14397;
RA   Yu M., Selvaraj S.K., Liang-Chu M.M.Y., Aghajani S., Busse M.,
RA   Yuan J., Lee G., Peale F.V., Klijn C., Bourgon R., Kaminker J.S.,
RA   Neve R.M.;
RT   "A resource for cell line authentication, annotation and quality
RT   control.";
RL   Nature 520:307-311(2015).
//
RX   PubMed=25926053; DOI=10.1038/ncomms8002;
RA   Medico E., Russo M., Picco G., Cancelliere C., Valtorta E., Corti G.,
RA   Buscarino M., Isella C., Lamba S., Martinoglio B., Veronese S.,
RA   Siena S., Sartore-Bianchi A., Beccuti M., Mottolese M.,
RA   Linnebacher M., Cordero F., Di Nicolantonio F., Bardelli A.;
RT   "The molecular landscape of colorectal cancer cell lines unveils
RT   clinically actionable kinase targets.";
RL   Nat. Commun. 6:7002.1-7002.10(2015).
//
RX   PubMed=25944804; DOI=10.1158/1078-0432.CCR-14-2457;
RA   Bazzocco S., Dopeso H., Carton-Garcia F., Macaya I., Andretta E.,
RA   Chionh F., Rodrigues P., Garrido M., Alazzouzi H., Nieto R.,
RA   Sanchez A., Schwartz S. Jr., Bilic J., Mariadason J.M., Arango D.;
RT   "Highly expressed genes in rapidly proliferating tumor cells as new
RT   targets for colorectal cancer treatment.";
RL   Clin. Cancer Res. 21:3695-3704(2015).
//
RX   PubMed=26537799; DOI=10.1074/mcp.M115.051235;
RA   Holst S., Deuss A.J.M., van Pelt G.W., van Vliet S.J.,
RA   Garcia-Vallejo J.J., Koeleman C.A.M., Deelder A.M., Mesker W.E.,
RA   Tollenaar R.A., Rombouts Y., Wuhrer M.;
RT   "N-glycosylation profiling of colorectal cancer cell lines reveals
RT   association of fucosylation with differentiation and caudal type
RT   homebox 1 (CDX1)/villin mRNA expression.";
RL   Mol. Cell. Proteomics 15:124-140(2016).
//
RX   PubMed=26589293; DOI=10.1186/s13073-015-0240-5;
RA   Scholtalbers J., Boegel S., Bukur T., Byl M., Goerges S., Sorn P.,
RA   Loewer M., Sahin U., Castle J.C.;
RT   "TCLP: an online cancer cell line catalogue integrating HLA type,
RT   predicted neo-epitopes, virus and gene expression.";
RL   Genome Med. 7:118.1-118.7(2015).
//
RX   PubMed=28196595; DOI=10.1016/j.ccell.2017.01.005;
RA   Li J., Zhao W., Akbani R., Liu W.-B., Ju Z.-L., Ling S.-Y., Vellano C.P.,
RA   Roebuck P., Yu Q.-H., Eterovic A.K., Byers L.A., Davies M.A., Deng W.-L.,
RA   Gopal Y.N.V., Chen G., von Euw E.M., Slamon D.J., Conklin D.,
RA   Heymach J.V., Gazdar A.F., Minna J.D., Myers J.N., Lu Y.-L., Mills G.B.,
RA   Liang H.;
RT   "Characterization of human cancer cell lines by reverse-phase protein
RT   arrays.";
RL   Cancer Cell 31:225-239(2017).
//
RX   PubMed=31068700; DOI=10.1038/s41586-019-1186-3;
RA   Ghandi M., Huang F.W., Jane-Valbuena J., Kryukov G.V., Lo C.C.,
RA   McDonald E.R. III, Barretina J.G., Gelfand E.T., Bielski C.M., Li H.-X.,
RA   Hu K., Andreev-Drakhlin A.Y., Kim J., Hess J.M., Haas B.J., Aguet F.,
RA   Weir B.A., Rothberg M.V., Paolella B.R., Lawrence M.S., Akbani R.,
RA   Lu Y.-L., Tiv H.L., Gokhale P.C., de Weck A., Mansour A.A., Oh C.,
RA   Shih J., Hadi K., Rosen Y., Bistline J., Venkatesan K., Reddy A.,
RA   Sonkin D., Liu M., Lehar J., Korn J.M., Porter D.A., Jones M.D.,
RA   Golji J., Caponigro G., Taylor J.E., Dunning C.M., Creech A.L.,
RA   Warren A.C., McFarland J.M., Zamanighomi M., Kauffmann A.,
RA   Stransky N., Imielinski M., Maruvka Y.E., Cherniack A.D.,
RA   Tsherniak A., Vazquez F., Jaffe J.D., Lane A.A., Weinstock D.M.,
RA   Johannessen C.M., Morrissey M.P., Stegmeier F., Schlegel R.,
RA   Hahn W.C., Getz G., Mills G.B., Boehm J.S., Golub T.R., Garraway L.A.,
RA   Sellers W.R.;
RT   "Next-generation characterization of the Cancer Cell Line
RT   Encyclopedia.";
RL   Nature 569:503-508(2019).
//
RX   PubMed=33389257; DOI=10.1007/s10096-020-04106-0;
RA   Wurtz N., Penant G., Jardot P., Duclos N., La Scola B.;
RT   "Culture of SARS-CoV-2 in a panel of laboratory cell lines,
RT   permissivity, and differences in growth profile.";
RL   Eur. J. Clin. Microbiol. Infect. Dis. 40:477-484(2021).
//