ID   Sf-9ET
AC   CVCL_1D71
SY   SF-9ET; Sf-9 Easy Titer; Sf-9 ET; Sf9-ET; Sf9-Easy Titer; Sf9 Easy Titer
DR   ATCC; CRL-3357
DR   Kerafast; ENH022-FP
DR   Wikidata; Q54952876
RX   PubMed=19852765;
WW   Provider; NCI TTC; TAB-4244/E-009-2008-0; https://techtransfer.cancer.gov/available-technologies?abstract=TAB-4244
WW   Provider; NIH OTT; E-009-2008/0-55; https://web.archive.org/web/20150922071005/http://www.ott.nih.gov/erma/development-sf-9et-easy-titer-inset-determining-baculovirus-titers
CC   Group: Insect cell line.
CC   Group: Recombinant protein production insect cell line.
CC   Characteristics: The expression of the eGFP gene is under the control of the baculovirus polyhedrin promoter.
CC   Doubling time: 28-32 hours (ATCC=CRL-3357).
CC   Genetic integration: Method=Transfection/transduction; Gene=FPbase; R9NL8; eGFP (Note=Enhanced GFP).
CC   Derived from site: In situ; Ovary; UBERON=UBERON_0000992.
OX   NCBI_TaxID=7108; ! Spodoptera frugiperda (Fall armyworm)
HI   CVCL_0549 ! Sf9
SX   Female
AG   Pupal stage
CA   Spontaneously immortalized cell line
DT   Created: 08-07-15; Last updated: 10-04-25; Version: 11
//
RX   PubMed=19852765; DOI=10.2144/000113238;
RA   Hopkins R.F. 3rd, Esposito D.;
RT   "A rapid method for titrating baculovirus stocks using the Sf-9 Easy
RT   Titer cell line.";
RL   BioTechniques 47:785-788(2009).
//