ID DU4475 AC CVCL_1183 SY Du4475; DU-4475; Du-4475; DU 4475; Du 4475; Duke University 4475 DR CLO; CLO_0002842 DR EFO; EFO_0002168 DR ArrayExpress; E-MTAB-38 DR ArrayExpress; E-MTAB-783 DR ArrayExpress; E-MTAB-2706 DR ArrayExpress; E-MTAB-2770 DR ArrayExpress; E-MTAB-3610 DR ATCC; CRL-7921 DR ATCC; HTB-123 DR BioGRID_ORCS_Cell_line; 684 DR BioSample; SAMN03471772 DR BioSample; SAMN03473595 DR BioSample; SAMN05292453 DR BioSample; SAMN10987660 DR cancercelllines; CVCL_1183 DR CCRID; 1101HUM-PUMC000342 DR Cell_Model_Passport; SIDM01001 DR ChEMBL-Cells; CHEMBL3308261 DR ChEMBL-Targets; CHEMBL2366246 DR CLS; 300371 DR Cosmic; 685863 DR Cosmic; 871141 DR Cosmic; 904355 DR Cosmic; 906844 DR Cosmic; 921975 DR Cosmic; 934523 DR Cosmic; 997915 DR Cosmic; 1046952 DR Cosmic; 1047716 DR Cosmic; 1091340 DR Cosmic; 1152520 DR Cosmic; 1287936 DR Cosmic; 1289387 DR Cosmic; 1609476 DR Cosmic; 2164988 DR Cosmic-CLP; 906844 DR DepMap; ACH-000258 DR DSMZ; ACC-427 DR DSMZCellDive; ACC-427 DR EGA; EGAS00001000610 DR EGA; EGAS00001000978 DR EGA; EGAS00001002554 DR GDSC; 906844 DR GEO; GSM217568 DR GEO; GSM274659 DR GEO; GSM344345 DR GEO; GSM344395 DR GEO; GSM421865 DR GEO; GSM783961 DR GEO; GSM886989 DR GEO; GSM888058 DR GEO; GSM1053707 DR GEO; GSM1669746 DR IARC_TP53; 21043 DR IGRhCellID; DU4475 DR LiGeA; CCLE_395 DR LINCS_HMS; 50577 DR LINCS_LDP; LCL-1323 DR PharmacoDB; DU4475_310_2019 DR PRIDE; PXD008222 DR PRIDE; PXD030304 DR Progenetix; CVCL_1183 DR PubChem_Cell_line; CVCL_1183 DR SLKBase; 3415 DR Wikidata; Q54831578 RX DOI=10.1016/B978-0-12-333530-2.50009-5; RX PubMed=221108; RX PubMed=10862037; RX PubMed=11343771; RX PubMed=11414198; RX PubMed=12068308; RX PubMed=15677628; RX PubMed=16397213; RX PubMed=16541312; RX PubMed=18397470; RX PubMed=19593635; RX PubMed=20164919; RX PubMed=20215515; RX PubMed=22460905; RX PubMed=23601657; RX PubMed=24094812; RX PubMed=25485619; RX PubMed=25877200; RX PubMed=25892236; RX PubMed=25984343; RX PubMed=26589293; RX PubMed=27397505; RX PubMed=28196595; RX PubMed=30894373; RX PubMed=31068700; RX PubMed=35839778; WW Info; LINCS; ICBP43; https://lincs.hms.harvard.edu/resources/reagents/icbp43/ WW Info; MCLP; -; https://tcpaportal.org/mclp/ WW Info; Pawefish; -; https://www.cellosaurus.org/pawefish/BreastCellLineDescriptions/DU4475.html WW Info; Synapse; JWGray panel; https://www.synapse.org/#!Synapse:syn2346643/wiki/62255 WW Info; Synapse; PS-ON; https://www.synapse.org/#!Synapse:syn31544500 CC Group: Triple negative breast cancer (TNBC) cell line. CC Part of: BRAF genetic alteration cell panel (ATCC TCP-1032). CC Part of: Cancer Dependency Map project (DepMap) (includes Cancer Cell Line Encyclopedia - CCLE). CC Part of: COSMIC cell lines project. CC Part of: ICBP43 breast cancer cell line panel. CC Part of: JWGray breast cancer cell line panel. CC Part of: MD Anderson Cell Lines Project. CC Part of: Naval Biosciences Laboratory (NBL) collection (transferred to ATCC in 1982). CC Population: Caucasian. CC Doubling time: 2.9 days (PubMed=221108); 90 hours (PubMed=25984343); ~80-100 hours (DSMZ=ACC-427); ~32 hours (PBCF). CC HLA typing: A*03:01,31:01; B*07:02,40:01; C*03:04,07:02 (PubMed=26589293). CC Microsatellite instability: Stable (MSS) (Sanger). CC Sequence variation: Gene deletion; HGNC; HGNC:6844; MAP2K4; Zygosity=Homozygous (PubMed=19593635). CC Sequence variation: Gene deletion; HGNC; HGNC:9884; RB1; Zygosity=Homozygous (PubMed=19593635). CC Sequence variation: Mutation; HGNC; HGNC:583; APC; Simple; p.Glu1577Ter (c.4729G>T); Zygosity=Homozygous (PubMed=19593635; ATCC=HTB-123). CC Sequence variation: Mutation; HGNC; HGNC:1097; BRAF; Simple; p.Val600Glu (c.1799T>A); ClinVar=VCV000013961; Zygosity=Heterozygous (PubMed=12068308; PubMed=18397470; PubMed=19593635; ATCC=HTB-123; Cosmic-CLP=906844; DepMap=ACH-000258). CC Omics: Genomics; DNA methylation analysis. CC Omics: Genomics; Whole exome sequencing. CC Omics: Phenotyping; Drug screening. CC Omics: Proteomics; Expression; Reverse-phase protein array. CC Omics: Proteomics; Quantitative. CC Omics: Transcriptomics; Microarray. CC Omics: Transcriptomics; miRNA profiling; Microarray. CC Omics: Transcriptomics; RNAseq. CC Omics: Variations; SNP array analysis. CC Genome ancestry: African=0.87%; Native American=0%; East Asian, North=3.81%; East Asian, South=0%; South Asian=0%; European, North=61.27%; European, South=34.04% (PubMed=30894373). CC Caution: Reported by some sites and publications to originate from a 62 year old woman, but the cell line was established when she was 70 year old. CC Misspelling: DU-475; Note=In text of PubMed 12702766. CC Misspelling: DU447; Cosmic=1609476. CC Discontinued: ATCC; CRL-7921; true. CC Derived from site: Metastatic; Skin; UBERON=UBERON_0002097. ST Source(s): ATCC=HTB-123; CCRID=1101HUM-PUMC000342; CLS=300371; Cosmic-CLP=906844; DSMZ=ACC-427; PubMed=25877200 ST Amelogenin: X ST CSF1PO: 9,12 ST D12S391: 18.3,25 ST D13S317: 11,14 ST D16S539: 11,12 ST D18S51: 14,16 ST D19S433: 14 ST D21S11: 29,31.2 ST D2S1338: 20,25 ST D3S1358: 14,16 ST D5S818: 6,11 (DSMZ=ACC-427) ST D5S818: 11 (ATCC=HTB-123; CCRID=1101HUM-PUMC000342; CLS=300371; Cosmic-CLP=906844; PubMed=25877200) ST D6S1043: 11 ST D7S820: 9,10 ST D8S1179: 10,13 ST FGA: 22,25 ST Penta D: 13,14 ST Penta E: 7,13 ST TH01: 6,8 ST TPOX: 8 ST vWA: 17 DI NCIt; C4872; Breast carcinoma OX NCBI_TaxID=9606; ! Homo sapiens (Human) SX Female AG 70Y CA Cancer cell line DT Created: 04-04-12; Last updated: 10-04-25; Version: 48 // RX DOI=10.1016/B978-0-12-333530-2.50009-5; RA Leibovitz A.; RT "Cell lines from human breast."; RL (In book chapter) Atlas of human tumor cell lines; Hay R.J., Park J.-G., Gazdar A.F. (eds.); pp.161-184; Academic Press; New York; USA (1994). // RX PubMed=221108; RA Langlois A.J., Holder W.D. Jr., Iglehart J.D., Nelson-Rees W.A., RA Wells S.A. Jr., Bolognesi D.P.; RT "Morphological and biochemical properties of a new human breast cancer RT cell line."; RL Cancer Res. 39:2604-2613(1979). // RX PubMed=10862037; DOI=10.1002/1098-2264(200007)28:3<308::AID-GCC9>3.0.CO;2-B; RA Kytola S., Rummukainen J., Nordgren A., Karhu R., Farnebo F., RA Isola J.J., Larsson C.; RT "Chromosomal alterations in 15 breast cancer cell lines by comparative RT genomic hybridization and spectral karyotyping."; RL Genes Chromosomes Cancer 28:308-317(2000). // RX PubMed=11343771; DOI=10.1016/S0165-4608(00)00387-3; RA Rummukainen J., Kytola S., Karhu R., Farnebo F., Larsson C., RA Isola J.J.; RT "Aberrations of chromosome 8 in 16 breast cancer cell lines by RT comparative genomic hybridization, fluorescence in situ RT hybridization, and spectral karyotyping."; RL Cancer Genet. Cytogenet. 126:1-7(2001). // RX PubMed=11414198; DOI=10.1007/s004320000207; RA Lahm H., Andre S., Hoeflich A., Fischer J.R., Sordat B., Kaltner H., RA Wolf E., Gabius H.-J.; RT "Comprehensive galectin fingerprinting in a panel of 61 human tumor RT cell lines by RT-PCR and its implications for diagnostic and RT therapeutic procedures."; RL J. Cancer Res. Clin. Oncol. 127:375-386(2001). // RX PubMed=12068308; DOI=10.1038/nature00766; RA Davies H.R., Bignell G.R., Cox C., Stephens P.J., Edkins S., Clegg S., RA Teague J.W., Woffendin H., Garnett M.J., Bottomley W., Davis N., RA Dicks E., Ewing R., Floyd Y., Gray K., Hall S., Hawes R., Hughes J., RA Kosmidou V., Menzies A., Mould C., Parker A., Stevens C., Watt S., RA Hooper S., Wilson R., Jayatilake H., Gusterson B.A., Cooper C.S., RA Shipley J.M., Hargrave D., Pritchard-Jones K., Maitland N.J., RA Chenevix-Trench G., Riggins G.J., Bigner D.D., Palmieri G., Cossu A., RA Flanagan A.M., Nicholson A., Ho J.W.C., Leung S.Y., Yuen S.T., RA Weber B.L., Seigler H.F., Darrow T.L., Paterson H.F., Marais R., RA Marshall C.J., Wooster R., Stratton M.R., Futreal P.A.; RT "Mutations of the BRAF gene in human cancer."; RL Nature 417:949-954(2002). // RX PubMed=15677628; DOI=10.1093/carcin/bgi032; RA Gorringe K.L., Chin S.-F., Pharoah P.D.P., Staines J.M., Oliveira C., RA Edwards P.A.W., Caldas C.; RT "Evidence that both genetic instability and selection contribute to RT the accumulation of chromosome alterations in cancer."; RL Carcinogenesis 26:923-930(2005). // RX PubMed=16397213; DOI=10.1158/0008-5472.CAN-05-2853; RA Elstrodt F., Hollestelle A., Nagel J.H.A., Gorin M., Wasielewski M., RA van den Ouweland A.M.W., Merajver S.D., Ethier S.P., Schutte M.; RT "BRCA1 mutation analysis of 41 human breast cancer cell lines reveals RT three new deleterious mutants."; RL Cancer Res. 66:41-45(2006). // RX PubMed=16541312; DOI=10.1007/s10549-006-9186-z; RA Wasielewski M., Elstrodt F., Klijn J.G.M., Berns E.M.J.J., Schutte M.; RT "Thirteen new p53 gene mutants identified among 41 human breast cancer RT cell lines."; RL Breast Cancer Res. Treat. 99:97-101(2006). // RX PubMed=18397470; DOI=10.1111/j.1749-0774.2008.00046.x; RA Ueda M., Toji E., Nunobiki O., Izuma S., Okamoto Y., Torii K., RA Noda S.; RT "Mutational analysis of the BRAF gene in human tumor cells."; RL Hum. Cell 21:13-17(2008). // RX PubMed=19593635; DOI=10.1007/s10549-009-0460-8; RA Hollestelle A., Nagel J.H.A., Smid M., Lam S., Elstrodt F., RA Wasielewski M., Ng S.S., French P.J., Peeters J.K., Rozendaal M.J., RA Riaz M., Koopman D.G., ten Hagen T.L.M., de Leeuw B.H.C.G.M., RA Zwarthoff E.C., Teunisse A.F.A.S., van der Spek P.J., Klijn J.G.M., RA Dinjens W.N.M., Ethier S.P., Clevers H.C., Jochemsen A.G., RA den Bakker M.A., Foekens J.A., Martens J.W.M., Schutte M.; RT "Distinct gene mutation profiles among luminal-type and basal-type RT breast cancer cell lines."; RL Breast Cancer Res. Treat. 121:53-64(2010). // RX PubMed=20164919; DOI=10.1038/nature08768; PMCID=PMC3145113; RA Bignell G.R., Greenman C.D., Davies H.R., Butler A.P., Edkins S., RA Andrews J.M., Buck G., Chen L., Beare D., Latimer C., Widaa S., RA Hinton J., Fahey C., Fu B.-Y., Swamy S., Dalgliesh G.L., Teh B.T., RA Deloukas P., Yang F.-T., Campbell P.J., Futreal P.A., Stratton M.R.; RT "Signatures of mutation and selection in the cancer genome."; RL Nature 463:893-898(2010). // RX PubMed=20215515; DOI=10.1158/0008-5472.CAN-09-3458; PMCID=PMC2881662; RA Rothenberg S.M., Mohapatra G., Rivera M.N., Winokur D., Greninger P., RA Nitta M., Sadow P.M., Sooriyakumar G., Brannigan B.W., Ulman M.J., RA Perera R.M., Wang R., Tam A., Ma X.-J., Erlander M., Sgroi D.C., RA Rocco J.W., Lingen M.W., Cohen E.E.W., Louis D.N., Settleman J., RA Haber D.A.; RT "A genome-wide screen for microdeletions reveals disruption of RT polarity complex genes in diverse human cancers."; RL Cancer Res. 70:2158-2164(2010). // RX PubMed=22460905; DOI=10.1038/nature11003; PMCID=PMC3320027; RA Barretina J.G., Caponigro G., Stransky N., Venkatesan K., Margolin A.A., RA Kim S., Wilson C.J., Lehar J., Kryukov G.V., Sonkin D., Reddy A., RA Liu M., Murray L., Berger M.F., Monahan J.E., Morais P., Meltzer J., RA Korejwa A., Jane-Valbuena J., Mapa F.A., Thibault J., Bric-Furlong E., RA Raman P., Shipway A., Engels I.H., Cheng J., Yu G.-Y.K., Yu J.-J., RA Aspesi P. Jr., de Silva M., Jagtap K., Jones M.D., Wang L., Hatton C., RA Palescandolo E., Gupta S., Mahan S., Sougnez C., Onofrio R.C., RA Liefeld T., MacConaill L.E., Winckler W., Reich M., Li N.-X., Mesirov J.P., RA Gabriel S.B., Getz G., Ardlie K., Chan V., Myer V.E., Weber B.L., RA Porter J., Warmuth M., Finan P., Harris J.L., Meyerson M.L., Golub T.R., RA Morrissey M.P., Sellers W.R., Schlegel R., Garraway L.A.; RT "The Cancer Cell Line Encyclopedia enables predictive modelling of RT anticancer drug sensitivity."; RL Nature 483:603-607(2012). // RX PubMed=23601657; DOI=10.1186/bcr3415; PMCID=PMC3672661; RA Riaz M., van Jaarsveld M.T.M., Hollestelle A., RA Prager-van der Smissen W.J.C., Heine A.A.J., Boersma A.W.M., Liu J.-J., RA Helmijr J.C.A., Ozturk B., Smid M., Wiemer E.A.C., Foekens J.A., RA Martens J.W.M.; RT "miRNA expression profiling of 51 human breast cancer cell lines RT reveals subtype and driver mutation-specific miRNAs."; RL Breast Cancer Res. 15:R33.1-R33.17(2013). // RX PubMed=24094812; DOI=10.1016/j.ccr.2013.08.020; PMCID=PMC3931310; RA Timmerman L.A., Holton T., Yuneva M., Louie R.J., Padro M., Daemen A., RA Hu M., Chan D.A., Ethier S.P., van 't Veer L.J., Polyak K., RA McCormick F., Gray J.W.; RT "Glutamine sensitivity analysis identifies the xCT antiporter as a RT common triple-negative breast tumor therapeutic target."; RL Cancer Cell 24:450-465(2013). // RX PubMed=25485619; DOI=10.1038/nbt.3080; RA Klijn C., Durinck S., Stawiski E.W., Haverty P.M., Jiang Z.-S., RA Liu H.-B., Degenhardt J., Mayba O., Gnad F., Liu J.-F., Pau G., RA Reeder J., Cao Y., Mukhyala K., Selvaraj S.K., Yu M.-M., Zynda G.J., RA Brauer M.J., Wu T.D., Gentleman R.C., Manning G., Yauch R.L., RA Bourgon R., Stokoe D., Modrusan Z., Neve R.M., de Sauvage F.J., RA Settleman J., Seshagiri S., Zhang Z.-M.; RT "A comprehensive transcriptional portrait of human cancer cell RT lines."; RL Nat. Biotechnol. 33:306-312(2015). // RX PubMed=25877200; DOI=10.1038/nature14397; RA Yu M., Selvaraj S.K., Liang-Chu M.M.Y., Aghajani S., Busse M., RA Yuan J., Lee G., Peale F.V., Klijn C., Bourgon R., Kaminker J.S., RA Neve R.M.; RT "A resource for cell line authentication, annotation and quality RT control."; RL Nature 520:307-311(2015). // RX PubMed=25892236; DOI=10.1016/j.celrep.2015.03.050; PMCID=PMC4425736; RA Lawrence R.T., Perez E.M., Hernandez D., Miller C.P., Haas K.M., RA Irie H.Y., Lee S.-I., Blau C.A., Villen J.; RT "The proteomic landscape of triple-negative breast cancer."; RL Cell Rep. 11:630-644(2015). // RX PubMed=25984343; DOI=10.1038/sdata.2014.35; PMCID=PMC4432652; RA Cowley G.S., Weir B.A., Vazquez F., Tamayo P., Scott J.A., Rusin S., RA East-Seletsky A., Ali L.D., Gerath W.F.J., Pantel S.E., Lizotte P.H., RA Jiang G.-Z., Hsiao J., Tsherniak A., Dwinell E., Aoyama S., Okamoto M., RA Harrington W., Gelfand E.T., Green T.M., Tomko M.J., Gopal S., RA Wong T.C., Li H.-B., Howell S., Stransky N., Liefeld T., Jang D., RA Bistline J., Meyers B.H., Armstrong S.A., Anderson K.C., RA Stegmaier K., Reich M., Pellman D., Boehm J.S., Mesirov J.P., RA Golub T.R., Root D.E., Hahn W.C.; RT "Parallel genome-scale loss of function screens in 216 cancer cell RT lines for the identification of context-specific genetic RT dependencies."; RL Sci. Data 1:140035.1-140035.12(2014). // RX PubMed=26589293; DOI=10.1186/s13073-015-0240-5; PMCID=PMC4653878; RA Scholtalbers J., Boegel S., Bukur T., Byl M., Goerges S., Sorn P., RA Loewer M., Sahin U., Castle J.C.; RT "TCLP: an online cancer cell line catalogue integrating HLA type, RT predicted neo-epitopes, virus and gene expression."; RL Genome Med. 7:118.1-118.7(2015). // RX PubMed=27397505; DOI=10.1016/j.cell.2016.06.017; PMCID=PMC4967469; RA Iorio F., Knijnenburg T.A., Vis D.J., Bignell G.R., Menden M.P., RA Schubert M., Aben N., Goncalves E., Barthorpe S., Lightfoot H., RA Cokelaer T., Greninger P., van Dyk E., Chang H., de Silva H., Heyn H., RA Deng X.-M., Egan R.K., Liu Q.-S., Mironenko T., Mitropoulos X., RA Richardson L., Wang J.-H., Zhang T.-H., Moran S., Sayols S., RA Soleimani M., Tamborero D., Lopez-Bigas N., Ross-Macdonald P., RA Esteller M., Gray N.S., Haber D.A., Stratton M.R., Benes C.H., RA Wessels L.F.A., Saez-Rodriguez J., McDermott U., Garnett M.J.; RT "A landscape of pharmacogenomic interactions in cancer."; RL Cell 166:740-754(2016). // RX PubMed=28196595; DOI=10.1016/j.ccell.2017.01.005; PMCID=PMC5501076; RA Li J., Zhao W., Akbani R., Liu W.-B., Ju Z.-L., Ling S.-Y., Vellano C.P., RA Roebuck P., Yu Q.-H., Eterovic A.K., Byers L.A., Davies M.A., Deng W.-L., RA Gopal Y.N.V., Chen G., von Euw E.M., Slamon D.J., Conklin D., RA Heymach J.V., Gazdar A.F., Minna J.D., Myers J.N., Lu Y.-L., Mills G.B., RA Liang H.; RT "Characterization of human cancer cell lines by reverse-phase protein RT arrays."; RL Cancer Cell 31:225-239(2017). // RX PubMed=30894373; DOI=10.1158/0008-5472.CAN-18-2747; PMCID=PMC6445675; RA Dutil J., Chen Z.-H., Monteiro A.N.A., Teer J.K., Eschrich S.A.; RT "An interactive resource to probe genetic diversity and estimated RT ancestry in cancer cell lines."; RL Cancer Res. 79:1263-1273(2019). // RX PubMed=31068700; DOI=10.1038/s41586-019-1186-3; PMCID=PMC6697103; RA Ghandi M., Huang F.W., Jane-Valbuena J., Kryukov G.V., Lo C.C., RA McDonald E.R. 3rd, Barretina J.G., Gelfand E.T., Bielski C.M., Li H.-X., RA Hu K., Andreev-Drakhlin A.Y., Kim J., Hess J.M., Haas B.J., Aguet F., RA Weir B.A., Rothberg M.V., Paolella B.R., Lawrence M.S., Akbani R., RA Lu Y.-L., Tiv H.L., Gokhale P.C., de Weck A., Mansour A.A., Oh C., RA Shih J., Hadi K., Rosen Y., Bistline J., Venkatesan K., Reddy A., RA Sonkin D., Liu M., Lehar J., Korn J.M., Porter D.A., Jones M.D., RA Golji J., Caponigro G., Taylor J.E., Dunning C.M., Creech A.L., RA Warren A.C., McFarland J.M., Zamanighomi M., Kauffmann A., RA Stransky N., Imielinski M., Maruvka Y.E., Cherniack A.D., RA Tsherniak A., Vazquez F., Jaffe J.D., Lane A.A., Weinstock D.M., RA Johannessen C.M., Morrissey M.P., Stegmeier F., Schlegel R., RA Hahn W.C., Getz G., Mills G.B., Boehm J.S., Golub T.R., Garraway L.A., RA Sellers W.R.; RT "Next-generation characterization of the Cancer Cell Line RT Encyclopedia."; RL Nature 569:503-508(2019). // RX PubMed=35839778; DOI=10.1016/j.ccell.2022.06.010; PMCID=PMC9387775; RA Goncalves E., Poulos R.C., Cai Z.-X., Barthorpe S., Manda S.S., Lucas N., RA Beck A., Bucio-Noble D., Dausmann M., Hall C., Hecker M., Koh J., RA Lightfoot H., Mahboob S., Mali I., Morris J., Richardson L., RA Seneviratne A.J., Shepherd R., Sykes E., Thomas F., Valentini S., RA Williams S.G., Wu Y.-X., Xavier D., MacKenzie K.L., Hains P.G., Tully B., RA Robinson P.J., Zhong Q., Garnett M.J., Reddel R.R.; RT "Pan-cancer proteomic map of 949 human cell lines."; RL Cancer Cell 40:835-849.e8(2022). //