ID   HMEEC-1
AC   CVCL_0E44
SY   HEI-HMEEC-1; HEI-HMEEC; Human Middle Ear Epithelial Cell line-1
DR   ATCC; CRL-13012
DR   BioSample; SAMN03471351
DR   Wikidata; Q54889922
RX   Patent=US6358688;
RX   PubMed=12090706;
RX   PubMed=21901615;
RX   PubMed=32433794;
CC   Group: Patented cell line.
CC   Registration: International Depositary Authority, American Type Culture Collection (ATCC); PTA-81.
CC   Doubling time: 23.8 hours (PubMed=12090706).
CC   Genetic integration: Method=Transduction; Gene=UniProtKB; P03126; Human papillomavirus type 16 protein E6.
CC   Genetic integration: Method=Transduction; Gene=UniProtKB; P03129; Human papillomavirus type 16 protein E7.
CC   Genetic integration: Method=Transduction; Gene=UniProtKB; P00552; Klebsiella pneumoniae transposon Tn5 neo.
CC   Transformant: NCBI_TaxID; 333760; Human papillomavirus type 16 (HPV16).
CC   Discontinued: ATCC; CRL-13012; true.
CC   Derived from site: In situ; Ear, middle ear; UBERON=UBERON_0001756.
CC   Cell type: Epithelial cell; CL=CL_0000066.
ST   Source(s): ATCC=CRL-13012
ST   Amelogenin: X,Y
ST   CSF1PO: 11
ST   D13S317: 10,11
ST   D16S539: 12,13
ST   D5S818: 10,13
ST   D7S820: 10,11
ST   TH01: 6,7
ST   TPOX: 9,11
ST   vWA: 16,17
OX   NCBI_TaxID=9606; ! Homo sapiens (Human)
SX   Male
AG   Age unspecified
CA   Transformed cell line
DT   Created: 10-04-15; Last updated: 14-08-25; Version: 16
//
RX   PubMed=32433794; DOI=10.1002/lary.28687;
RA   Espahbodi, Mana
RA   Samuels, Tina L.
RA   McCormick, Caroline
RA   Khampang, Pawjai
RA   Yan, Ke
RA   Marshall, Samuel
RA   McCormick, Michael E.
RA   Chun, Robert H.
RA   Harvey, Steven A.
RA   Friedland, David Richard
RA   Johnston, Nikki
RA   Kerschner, Joseph E.
RT   "Analysis of inflammatory signaling in human middle ear cell culture
RT   models of pediatric otitis media.";
RL   Laryngoscope 131:410-416(2021).
//
RX   Patent=US6358688;
RA   Lim, David J.
RA   Chun, Young-Myoung
RA   Rhim, Johng Sik
RT   "Immortalized human middle ear epithelial cell lines.";
RL   Patent number US6358688, 19-Mar-2002.
//
RX   PubMed=12090706; DOI=10.1177/000348940211100606;
RA   Chun, Young-Myoung
RA   Moon, Sung-Kyun
RA   Lee, Haa-Yung
RA   Webster, Paul
RA   Brackmann, Derald Elmer
RA   Rhim, Johng Sik
RA   Lim, David J.
RT   "Immortalization of normal adult human middle ear epithelial cells
RT   using a retrovirus containing the E6/E7 genes of human papillomavirus
RT   type 16.";
RL   Ann. Otol. Rhinol. Laryngol. 111:507-517(2002).
//
RX   PubMed=21901615; DOI=10.1007/978-1-4614-0254-1_2;
RA   Lim, David J.
RA   Moon, Sung-Kyun
RT   "Establishment of cell lines from the human middle and inner ear
RT   epithelial cells.";
RL   Adv. Exp. Med. Biol. 720:15-25(2011).
//