Cellosaurus WTC FBL-mEGFP/NPM1-mTagRFPT/CLYBL-dCas9-TagBFP-KRAB-cl61 (CVCL_YU29)
Cell line name | WTC FBL-mEGFP/NPM1-mTagRFPT/CLYBL-dCas9-TagBFP-KRAB-cl61 |
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Synonyms | AICS-0089-061; AICSi002-A-39; UCSFi001-A-39 |
Accession | CVCL_YU29 |
Resource Identification Initiative | To cite this cell line use: WTC FBL-mEGFP/NPM1-mTagRFPT/CLYBL-dCas9-TagBFP-KRAB-cl61 (RRID:CVCL_YU29) |
Comments | Part of: Allen Cell Collection. Population: Japanese. Characteristics: Using CRISPR/Cas9 FBL has been endogenously tagged in one allele at the C-terminus with mEGFP, NPM1 has been endogenously tagged in one allele at the C-terminus with mTagRFP-T and a dCas9-TagBFP-KRAB construct is expressed from a CAGGS promoter edited into the 2nd intron of the CLYBL safe harbor. Genetic integration: Method=CRISPR/Cas9; Gene=FPbase; BFJKS; TagBFP (Note=Blue fluorescent protein derived from eqFP578). Genetic integration: Method=CRISPR/Cas9; Gene=FPbase; LF3LJ; TagRFP-T (Note=Red fluorescent protein derived from eqFP578). Genetic integration: Method=CRISPR/Cas9; Gene=UniProtKB; Q99ZW2; Streptomyces pyogenes Cas9 (Note=With p.Asp10Ala and p.His840Ala and KRAB domain = dCas9-KRAB). Derived from site: In situ; Leg, skin; UBERON=UBERON_0001511. Cell type: Fibroblast of skin; CL=CL_0002620. |
Species of origin | Homo sapiens (Human) (NCBI Taxonomy: 9606) |
Hierarchy | Parent: CVCL_Y803 (GM25256) |
Sex of cell | Male |
Age at sampling | 30Y |
Category | Induced pluripotent stem cell |
Web pages | https://www.allencell.org/cell-catalog.html |
Cross-references | |
Cell line collections (Providers) | Coriell; AICS-0089-061 |
Cell line databases/resources | hPSCreg; UCSFi001-A-39 |
Biological sample resources | BioSamples; SAMEA7655481 |
Encyclopedic resources | Wikidata; Q98135041 |
Entry history | |
Entry creation | 12-Mar-2020 |
Last entry update | 10-Sep-2024 |
Version number | 11 |