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Cellosaurus CynESC-OTZ4 (CVCL_WJ35)

[Text version]
Cell line name CynESC-OTZ4
Synonyms OTZ4
Accession CVCL_WJ35
Resource Identification Initiative To cite this cell line use: CynESC-OTZ4 (RRID:CVCL_WJ35)
Comments Group: Non-human primate cell line.
Characteristics: Using CRISPR/Cas9 the stop codon of POU5F1 was replaced with a P2A-tdTomato-IRES-ZeocinR construct.
Genetic integration: Method=CRISPR/Cas9; Gene=FPbase; PGG5S; tdTomato (Note=Bright red fluorescent protein derived from drFP583 (DsRed)).
Genetic integration: Method=CRISPR/Cas9; Gene=UniProtKB; P17493; Streptoalloteichus hindustanus ble (BleoR).
Derived from site: In situ; Blastocyst; UBERON=UBERON_0000358.
Cell type: Embryonic stem cell; CL=CL_0002322.
Species of origin Macaca fascicularis (Crab-eating macaque) (Cynomolgus monkey) (NCBI Taxonomy: 9541)
Hierarchy Parent: CVCL_WJ34 (CynESC-3X)
Sex of cell Female
Age at sampling Blastocyst stage
Category Embryonic stem cell
Publications

PubMed=31005787; DOI=10.1016/j.scr.2019.101439
Kobayashi K., Tsukiyama T., Nakaya M., Kageyama S., Tomita K., Murai R., Yoshida T., Narita M., Kawauchi A., Ema M.
Generation of an OCT3/4 reporter cynomolgus monkey ES cell line using CRISPR/Cas9.
Stem Cell Res. 37:101439-101439(2019)

Cross-references
Encyclopedic resources Wikidata; Q93465204
Entry history
Entry creation24-May-2019
Last entry update10-Sep-2024
Version number7