ID   CH-RPE.11a
AC   CVCL_D3WW
SY   RPE.11a
RX   PubMed=494060;
RX   PubMed=7559470;
CC   Characteristics: This is a class II Pseudomonas toxin resistant cell line. The resistance of class II mutants cannot be overcome by increased concentration of the toxin. They possess a mutationally altered EF-2 which does not serve as a substrate for ADP-ribosylation by the Pseudomonas toxin (PubMed=494060).
CC   Selected for resistance to: UniProtKB; P11439; Pseudomonas aeruginosa exotoxin (eta).
CC   Sequence variation: Mutation; UniProtKB; P09445; EEF2; Simple; p.Ser584Gly (c.1750A>G); Zygosity=Heterozygous (PubMed=7559470).
CC   Transformant: ChEBI; CHEBI:23994; Ethyl methanesulfonate (Methylsulfonic acid ethyl ester; MSEE).
CC   Derived from site: In situ; Ovary; UBERON=UBERON_0000992.
CC   Cell type: Epithelial cell of ovary; CL=CL_2000064.
OX   NCBI_TaxID=10029; ! Cricetulus griseus (Chinese hamster)
HI   CVCL_0214 ! CHO-K1
SX   Female
AG   Adult
CA   Spontaneously immortalized cell line
DT   Created: 30-01-24; Last updated: 30-01-24; Version: 1
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RX   PubMed=494060; DOI=10.1007/BF01538880;
RA   Moehring J.M., Moehring T.J.;
RT   "Characterization of the diphtheria toxin-resistance system in Chinese
RT   hamster ovary cells.";
RL   Somatic Cell Genet. 5:453-468(1979).
//
RX   PubMed=7559470; DOI=10.1074/jbc.270.39.23218;
RA   Foley B.T., Moehring J.M., Moehring T.J.;
RT   "Mutations in the elongation factor 2 gene which confer resistance to
RT   diphtheria toxin and Pseudomonas exotoxin A. Genetic and biochemical
RT   analyses.";
RL   J. Biol. Chem. 270:23218-23225(1995).
//