Cellosaurus cell line MCF7/Cas9 AAVS1 (CVCL

Cross-references
Cell line name	MCF7/Cas9 AAVS1
Accession	CVCL_C9CD
Resource Identification Initiative	To cite this cell line use: MCF7/Cas9 AAVS1 (RRID:CVCL_C9CD)
Comments	Population: Caucasian. Characteristics: Stably expresses a CBh-3xFLAG-Cas9-NLS-bGHPolyA-EF1a-copGFP-T2A-HygR-SV40pa construct from the AAVS1 safe locus (GeneCopoeia=SL514). Transfected with: UniProtKB; Q99ZW2; Streptomyces pyogenes Cas9. Transfected with: UniProtKB; Q6WV12; Pontellina plumata GFP2 (copGFP). Transfected with: UniProtKB; P00557; Escherichia coli hygromycin-B 4-O-kinase (hph) (HygR). Derived from site: Metastatic; Pleural effusion; UBERON=UBERON_0000175.
Sequence variations	Gene deletion; HGNC; 1787; CDKN2A; Zygosity=Homozygous (from parent cell line). Mutation; HGNC; 4172; GATA3; Simple; p.Asp336Glyfs*17 (c.1006dupG); Zygosity=Heterozygous (from parent cell line). Mutation; HGNC; 8975; PIK3CA; Simple; p.Glu545Lys (c.1633G>A); ClinVar=VCV000013655; Zygosity=Heterozygous (from parent cell line).
Disease	Invasive breast carcinoma of no special type (NCIt: C4194)
Species of origin	Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy	Parent: CVCL_0031 (MCF-7)
Sex of cell	Female
Age at sampling	69Y
Category	Cancer cell line
Cell line collections (Providers)	GeneCopoeia; SL514
Encyclopedic resources	Wikidata; Q123033008
Entry history
Entry creation	05-Oct-2023
Last entry update	30-Jan-2024
Version number	2