ID   NRel-4
AC   CVCL_C8BV
DR   Wikidata; Q123033197
RX   PubMed=9576878;
CC   Characteristics: Defective in dihydroxyacetone phosphate acyltransferase (PubMed=9576878).
CC   Characteristics: Selected using a two step protocol, first using the 9-(1'-pyrene)nonanol/ultraviolet (P9OH/UV) method and then two rounds of 12-(1'-pyrene)dodecanoic acid/ultraviolter (P12/UV) selection. P9OH acts as a photosensitizer. Normal cells which can incorporate the fluorescent fatty alcohol into lipids are killed by UV, but mutants who incorporate P9OH poorly are less susceptible to photosensitized killing (PubMed=9576878).
CC   Transformant: ChEBI; CHEBI:23994; Ethyl methanesulfonate (Methylsulfonic acid ethyl ester; MSEE).
CC   Derived from site: In situ; Ovary; UBERON=UBERON_0000992.
CC   Cell type: Epithelial cell of ovary; CL=CL_2000064.
OX   NCBI_TaxID=10029; ! Cricetulus griseus (Chinese hamster)
HI   CVCL_0214 ! CHO-K1
SX   Female
AG   Adult
CA   Transformed cell line
DT   Created: 29-06-23; Last updated: 30-01-24; Version: 2
//
RX   PubMed=9576878; DOI=10.1042/bj3320273;
RA   Nagan N., Hajra A.K., Larkins L.K., Lazarow P., Purdue P.E.,
RA   Rizzo W.B., Zoeller R.A.;
RT   "Isolation of a Chinese hamster fibroblast variant defective in
RT   dihydroxyacetonephosphate acyltransferase activity and plasmalogen
RT   biosynthesis: use of a novel two-step selection protocol.";
RL   Biochem. J. 332:273-279(1998).
//