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Cellosaurus ML-DmBG2-c2-attP-25C6 (CVCL_C7G2)

[Text version]
Cell line name ML-DmBG2-c2-attP-25C6
Synonyms ML-DmBG2-c2-attP-25C6-Clone 8
Accession CVCL_C7G2
Resource Identification Initiative To cite this cell line use: ML-DmBG2-c2-attP-25C6 (RRID:CVCL_C7G2)
Comments Group: Insect cell line.
Characteristics: Using CRISPR/Cas9 a Actin5C promoter-dsRed cassette flanked by attP sites was introduced in the 25C6 locus (PubMed=33963853).
Genetic integration: Method=CRISPR/Cas9; Gene=FPbase; PGG5S; tdTomato (Note=Bright red fluorescent protein derived from drFP583 (DsRed)).
Species of origin Drosophila melanogaster (Fruit fly) (NCBI Taxonomy: 7227)
Hierarchy Parent: CVCL_Z719 (ML-DmBG2-c2)
Sex of cell Male
Age at sampling Third instar larval stage
Category Spontaneously immortalized cell line
Publications

PubMed=33963853; DOI=10.1093/g3journal/jkab161; PMCID=PMC8496291
Mariyappa D., Luhur A., Overton D., Zelhof A.C.
Generation of Drosophila attP containing cell lines using CRISPR-Cas9.
G3 (Bethesda) 11:jkab161.1-jkab161.8(2021)

Cross-references
Cell line collections (Providers) DGRC; 319
Cell line databases/resources FlyBase_Cell_line; FBtc0000319
Encyclopedic resources Wikidata; Q117704711
Entry history
Entry creation21-Mar-2023
Last entry update10-Sep-2024
Version number4