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Cellosaurus MCF-7/PacR (CVCL_B7P7)

[Text version]
Cell line name MCF-7/PacR
Synonyms MCF7/PacR; MCF-7/PAC
Accession CVCL_B7P7
Resource Identification Initiative To cite this cell line use: MCF-7/PacR (RRID:CVCL_B7P7)
Comments Population: Caucasian.
Characteristics: Generated by step-increasing drug concentration to 300 nm of paclitaxel. Overexpresses ABCB1 which is amplified and inserted in chromosome 3 (PubMed=27664577; Direct_author_submission).
Doubling time: ~25-30 hours (Direct_author_submission).
Selected for resistance to: ChEBI; CHEBI:45863; Paclitaxel (Taxol).
Omics: Mitochondrial proteome analysis by 2D-DE/MS.
Miscellaneous: Doubling time, ABCB1 status and STR profile from personal communication of Daniel P.
Derived from site: Metastatic; Pleural effusion; UBERON=UBERON_0000175.
Sequence variations
  • Gene deletion; HGNC; 1787; CDKN2A; Zygosity=Homozygous (from parent cell line).
  • Mutation; HGNC; 4172; GATA3; Simple; p.Asp336Glyfs*17 (c.1006dupG); Zygosity=Heterozygous (from parent cell line).
  • Mutation; HGNC; 8975; PIK3CA; Simple; p.Glu545Lys (c.1633G>A); ClinVar=VCV000013655; Zygosity=Heterozygous (from parent cell line).
Disease Invasive breast carcinoma of no special type (NCIt: C4194)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_0031 (MCF-7)
Sex of cell Female
Age at sampling 69Y
Category Cancer cell line
STR profile Source(s): Direct_author_submission

Markers:
AmelogeninX
CSF1PO10
D2S133821,23
D3S135816
D5S81811,12
D7S8209
D8S117910,14
D13S31711
D16S53911,12
D18S5114
D19S43313,14
D21S1130
FGA23,25
TH016
TPOX9,12
vWA14,15

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Publications

PubMed=27664577; DOI=10.1016/j.taap.2016.09.020
Nemcova-Furstova V., Kopperova D., Balusikova K., Ehrlichova M., Brynychova V., Vaclavikova R., Daniel P., Soucek P., Kovar J.
Characterization of acquired paclitaxel resistance of breast cancer cells and involvement of ABC transporters.
Toxicol. Appl. Pharmacol. 310:215-228(2016)

PubMed=29625142; DOI=10.1016/j.taap.2018.04.002
Jelinek M., Balusikova K., Daniel P., Nemcova-Furstova V., Kirubakaran P., Jacek M., Wei L.-F., Wang X., Vondrasek J., Ojima I., Kovar J.
Substituents at the C3' and C3'N positions are critical for taxanes to overcome acquired resistance of cancer cells to paclitaxel.
Toxicol. Appl. Pharmacol. 347:79-91(2018)

PubMed=31248089; DOI=10.3390/ijms20122986
Daniel P., Halada P., Jelinek M., Balusikova K., Kovar J.
Differentially expressed mitochondrial proteins in human MCF7 breast cancer cells resistant to paclitaxel.
Int. J. Mol. Sci. 20:2986.1-2986.20(2019)

Cross-references
Cell line databases/resources cancercelllines; CVCL_B7P7
Encyclopedic resources Wikidata; Q112930090
Entry history
Entry creation23-Jun-2022
Last entry update05-Oct-2023
Version number5