Cellosaurus Sua-5B-IE8-Act::Cas9-2A-Neo (CVCL_B3N3)
Cell line name | Sua-5B-IE8-Act::Cas9-2A-Neo |
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Accession | CVCL_B3N3 |
Resource Identification Initiative | To cite this cell line use: Sua-5B-IE8-Act::Cas9-2A-Neo (RRID:CVCL_B3N3) |
Comments | Group: Insect cell line. Group: Malaria research cell line. Characteristics: Modified using a Minos mediated integration cassette (MiMIC) to deliver a recombination-mediated cassette (RMCE) acceptor, to add a docking site for recombination via the bacteriophage FC31 integrase. Characteristics: A N-3xFLAG-spCas9-T2A-Neo construct was integrated in the Act5C locus (PubMed=34819517). Doubling time: ~20 hours (DGRC=334). Genetic integration: Method=Transfection; Gene=FPbase; ZERB6; mCherry (Note=Red fluorescent protein derived from drFP583 (dsRed)). Genetic integration: Method=Transfection; Gene=UniProtKB; Q99ZW2; Streptomyces pyogenes Cas9. Genetic integration: Method=Transfection; Gene=UniProtKB; P00552; Transposon Tn5 neo. |
Species of origin | Anopheles coluzzii (African malaria mosquito)
(NCBI Taxonomy: 1518534)
Breed/subspecies: Suakoko 2La. |
Hierarchy | Parent: CVCL_B3N2 (Sua-5B-IE8) |
Sex of cell | Sex unspecified |
Age at sampling | Neonate larva |
Category | Spontaneously immortalized cell line |
Publications | PubMed=34819517; DOI=10.1038/s41467-021-27129-3; PMCID=PMC8613219 |
Cross-references | |
Cell line collections (Providers) | DGRC; 334 |
Encyclopedic resources | Wikidata; Q110433264 |
Entry history | |
Entry creation | 16-Dec-2021 |
Last entry update | 10-Sep-2024 |
Version number | 7 |