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Cellosaurus M4A4 LM3-2 GFP (CVCL_B059)

[Text version]
Cell line name M4A4 LM3-2 GFP
Accession CVCL_B059
Resource Identification Initiative To cite this cell line use: M4A4 LM3-2 GFP (RRID:CVCL_B059)
Comments Problematic cell line: Contaminated. Ancestral cell line (MDA-MB-435) has been shown to be a M14 derivative.
Registration: International Cell Line Authentication Committee, Register of Misidentified Cell Lines; ICLAC-00562.
Population: Caucasian.
Transfected with: UniProtKB; P42212; GFP.
Transfected with: UniProtKB; P00552; Transposon Tn5 neo.
Derived from site: Metastatic; Right buttock, hypodermis; UBERON=UBERON_0013691+UBERON_0002072.
Sequence variations
  • Mutation; HGNC; 1097; BRAF; Simple; p.Val600Glu (c.1799T>A); ClinVar=VCV000013961; Zygosity=Heterozygous (from parent cell line).
  • Mutation; HGNC; 1787; CDKN2A; Simple; c.150+2T>C (IVS1+2T>C); ClinVar=VCV000406712; Zygosity=Heterozygous; Note=Splice donor mutation (from parent cell line).
  • Mutation; HGNC; 1787; CDKN2A; Simple; c.455insCdel26; Zygosity=Heterozygous (from parent cell line).
  • Mutation; HGNC; 11998; TP53; Simple; p.Gly266Glu (c.797G>A); ClinVar=VCV000161516; Zygosity=Heterozygous (from parent cell line).
Disease Amelanotic melanoma (NCIt: C3802)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_B058 (M4A4 GFP)
Sex of cell Male
Age at sampling 33Y
Category Cancer cell line
STR profile Source(s): ATCC=CRL-2916

Markers:
AmelogeninX
CSF1PO11
D2S133819,24
D3S135814
D5S81811,12
D7S8208,10
D8S117913
D13S31712
D16S53913
D18S5113,17
D19S43314
D21S1130
FGA21
Penta D9,11
Penta E10,12
TH016,7
TPOX8,11
vWA16,18

Run an STR similarity search on this cell line
Web pages https://www.atcc.org/en/support/technical-support/faqs/atcc-htb-129-derivative
https://iclac.org/wp-content/uploads/Cross-Contaminations_v13_distribution.xlsx
Cross-references
Cell line collections (Providers) ATCC; CRL-2916
Cell line databases/resources cancercelllines; CVCL_B059
Encyclopedic resources Wikidata; Q54903624
Entry history
Entry creation06-Jun-2012
Last entry update02-May-2024
Version number26