Cellosaurus logo
expasy logo

Cellosaurus Kc167-attP-25C6 (CVCL_A5US)

[Text version]
Cell line name Kc167-attP-25C6
Synonyms Kc167-attP-25C6-; Kc167-attP-25C6-Clone 35; Kc167-attP-25C6-Clone35
Accession CVCL_A5US
Resource Identification Initiative To cite this cell line use: Kc167-attP-25C6 (RRID:CVCL_A5US)
Comments Group: Insect cell line.
Characteristics: Using CRISPR/Cas9 a Actin5C promoter-dsRed cassette flanked by attP sites was introduced in the 25C6 locus (PubMed=33963853).
Genetic integration: Method=CRISPR/Cas9; Gene=FPbase; PGG5S; tdTomato (Note=Bright red fluorescent protein derived from drFP583 (DsRed)).
Species of origin Drosophila melanogaster (Fruit fly) (NCBI Taxonomy: 7227)
Hierarchy Parent: CVCL_Z834 (Kc167)
Sex of cell Female
Age at sampling Dorsal closure stage
Category Spontaneously immortalized cell line
Publications

PubMed=33963853; DOI=10.1093/g3journal/jkab161; PMCID=PMC8496291
Mariyappa D., Luhur A., Overton D., Zelhof A.C.
Generation of Drosophila attP containing cell lines using CRISPR-Cas9.
G3 (Bethesda) 11:jkab161.1-jkab161.8(2021)

Cross-references
Cell line collections (Providers) DGRC; 317
Cell line databases/resources FlyBase_Cell_line; FBtc0000317
Encyclopedic resources Wikidata; Q107115534
Entry history
Entry creation20-May-2021
Last entry update10-Sep-2024
Version number6