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Cellosaurus CHO-DG44 (CVCL_7180)

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Cell line name CHO-DG44
Synonyms CHO DG44; CHO-DG44 (DHFR-); CHO DG-44; DG-44; DG44
Accession CVCL_7180
Resource Identification Initiative To cite this cell line use: CHO-DG44 (RRID:CVCL_7180)
Comments Doubling time: 24.4 +- 0.68 hours (PubMed=21345355).
Selected for resistance to: ChEBI; CHEBI_44185; Methotrexate (MTX).
Transformant: NCIt; C129640; Cobalt-60 gamma radiation.
Omics: Genomics; Whole genome sequencing.
Omics: Proteomics.
Derived from site: In situ; Ovary; UBERON=UBERON_0000992.
Cell type: Epithelial cell of ovary; CL=CL_2000064.
Sequence variations
  • Gene deletion; UniProtKB; Q2MH30; Dhfr; Zygosity=Homozygous (PubMed=6305508).
Species of origin Cricetulus griseus (Chinese hamster) (Cricetulus barabensis griseus) (NCBI Taxonomy: 10029)
Hierarchy Parent: CVCL_7182 (CHO Pro-3)
Children:
CVCL_A4AR (Agarabi CHO)CVCL_VU03 (CHO B8-300)CVCL_VU65 (CHO E1a)
CVCL_E3EJ (CHO TSHR-0)CVCL_E3EN (CHO TSHR-289)CVCL_E3EK (CHO TSHR-WT)
CVCL_KA66 (CHO-DG44 (cGMP banked))CVCL_E3EH (CHO-TPO-2B)CVCL_E3EI (CHO-TPO-C4C,)
CVCL_E3EG (CHO-TPO-M1-K1)CVCL_D3PI (CHO/ASCT2)CVCL_B0VY (CHO/CERT 2.20)
CVCL_B0VZ (CHO/CERT 2.41)CVCL_D3PM (CHO/CLDN3)CVCL_D3PN (CHO/CLDN4)
CVCL_D3PP (CHO/CLDN5)CVCL_D3PQ (CHO/CLDN6)CVCL_D3PR (CHO/CLDN9)
CVCL_YY02 (CHOmt17-0)CVCL_WB19 (CHOpper Discovery)CVCL_WB20 (CHOpper Standard)
CVCL_5A35 (haAQC2)CVCL_5A34 (hAQC2)CVCL_5A36 (hsAQC2)
CVCL_KA70 (Ms704)CVCL_KA71 (MS705)CVCL_KA72 (Ms709)
CVCL_IR07 (NeuCHO)
Sex of cell Female
Age at sampling Adult
Category Transformed cell line
Web pages Info; Thermo Fisher; -; https://www.thermofisher.com/ch/en/home/technical-resources/cell-lines/c/cell-lines-detail-404.html
Info; Wikipedia; -; https://en.wikipedia.org/wiki/Chinese_hamster_ovary_cell
Publications

PubMed=6305508; DOI=10.1016/0092-8674(83)90422-1
Gail Urlaub, Emmanuel Kas, Adelaide M. Carothers, Lawrence A. Chasin;
Deletion of the diploid dihydrofolate reductase locus from cultured mammalian cells.
Cell 33:405-412(1983)

PubMed=16403443; DOI=10.1016/j.bbrc.2005.12.111
Madiha Derouazi, Danielle Martinet, Nathalie Besuchet-Schmutz, Rachel Flaction, Marianne Wicht, Martin Bertschinger, David Lynn Hacker, Jacques S. Beckmann, Florian Maria Wurm;
Genetic characterization of CHO production host DG44 and derivative recombinant cell lines.
Biochem. Biophys. Res. Commun. 340:1069-1077(2006)

DOI=10.1007/978-1-4020-5476-1_59
Danielle Martinet, Madiha Derouazi, Nathalie Besuchet-Schmutz, Marianne Wicht, Jacques S. Beckmann, Florian Maria Wurm;
Karyotype of CHO DG44 cells.
(In book chapter) Cell technology for cell products. Proceedings of the 19th ESACT Meeting; Smith, Rodney (eds.); pp.363-366; Springer; Dordrecht; Netherlands (2007)

PubMed=21345355; DOI=10.1016/j.jbiotec.2011.02.002
Lore Florin, Carolin Lipske, Eric Becker, Hitto Kaufmann;
Supplementation of serum free media with HT is not sufficient to restore growth properties of DHFR-/- cells in fed-batch processes -- implications for designing novel CHO-based expression platforms.
J. Biotechnol. 152:189-193(2011)

DOI=10.3390/pr1030296
Florian Maria Wurm;
CHO quasispecies -- implications for manufacturing processes.
Processes 1:296-311(2013)

PubMed=23873082; DOI=10.1038/nbt.2624
Nathan E. Lewis, Xin Liu, Yu-Xiang Li, Harish Nagarajan, George Yerganian, Edward O'Brien, Aarash Bordbar, Anne M. Roth, Jeffrey Rosenbloom, Chao Bian, Min Xie ...Show all 21 authors... , Wen-Bin Chen, Ning Li, Deniz Baycin-Hizal, Haythem Latif, Jochen Forster, Michael J. Betenbaugh, Iman Famili, Xun Xu, Jun Wang, Bernhard Orn Palsson; Show fewer authors
Genomic landscapes of Chinese hamster ovary cell lines as revealed by the Cricetulus griseus draft genome.
Nat. Biotechnol. 31:759-765(2013)

DOI=10.3390/pr5020020
Florian Maria Wurm, Maria Joao Wurm;
Cloning of CHO cells, productivity and genetic stability -- a discussion.
Processes 5:20.1-20.13(2017)

PubMed=28876938; DOI=10.1021/acs.jproteome.7b00382
Kelley M. Heffner, Deniz Baycin-Hizal, George Yerganian, Amit Kumar, Ozge Can, Robert N. O'Meally, Robert Norman Cole, Raghothama Chaerkady, Herren Wu, Michael A. Bowen, Michael J. Betenbaugh;
Lessons from the hamster: Cricetulus griseus tissue and CHO cell line proteome comparison.
J. Proteome Res. 16:3672-3687(2017)

PubMed=29987698; DOI=10.1007/s10616-018-0233-5; PMCID=PMC6214854
Shuichi Kimura, Takeshi Omasa;
Genome sequence comparison between Chinese hamster ovary (CHO) DG44 cells and mouse using end sequences of CHO BAC clones based on BAC-FISH results.
Cytotechnology 70:1399-1407(2018)

PubMed=32619503; DOI=10.1016/j.ymben.2020.06.002
Diana Szeliova, David Erich Ruckerbauer, Sarah Noel Galleguillos, Lars B. Petersen, Klaus Natter, Michael Hanscho, Christina Troyer, Tim Causon, Harald Schoeny, Hanne Bjerre Christensen, Dong-Yup Lee ...Show all 17 authors... , Nathan E. Lewis, Gunda Koellensperger, Stephan Hann, Lars K. Nielsen, Nicole Borth, Jurgen Zanghellini; Show fewer authors
What CHO is made of: variations in the biomass composition of Chinese hamster ovary cell lines.
Metab. Eng. 61:288-300(2020)

PubMed=32985598; DOI=10.1038/s41598-020-72959-8; PMCID=PMC7522264
Kelley M. Heffner, Deniz Baycin-Hizal, Natalia I. Majewska, Swetha Kumar, Venkata Gayatri Dhara, Jie Zhu, Michael A. Bowen, Diane Hatton, George Yerganian, Athena Yerganian, Robert N. O'Meally ...Show all 13 authors... , Robert Norman Cole, Michael J. Betenbaugh; Show fewer authors
Expanded Chinese hamster organ and cell line proteomics profiling reveals tissue-specific functionalities.
Sci. Rep. 10:15841.1-15841.12(2020)

PubMed=34050613; DOI=10.1002/biot.202100165
Maria Joao Wurm, Florian Maria Wurm;
Naming CHO cells for bio-manufacturing: genome plasticity and variant phenotypes of cell populations in bioreactors question the relevance of old names.
Biotechnol. J. 16:e2100165.1-e2100165.13(2021)

Cross-references
Cell line collections (Providers) NCBI_Iran; C576
Cell line databases/resources Lonza; 467
Lonza; 468
TOKU-E; 3678
Encyclopedic resources Wikidata; Q54812671
Entry history
Entry creation04-Apr-2012
Last entry update10-Apr-2025
Version number28