ID   MGC-803
AC   CVCL_5334
SY   MGC803; MGc80-3
DR   BTO; BTO:0004953
DR   EFO; EFO_0006286
DR   ChEMBL-Cells; CHEMBL3706567
DR   ChEMBL-Targets; CHEMBL3706566
DR   Cosmic; 2267321
DR   GEO; GSM844588
DR   GEO; GSM1374671
DR   IARC_TP53; 24858
DR   PubChem_Cell_line; CVCL_5334
DR   Ubigene; YC-C081
DR   Wikidata; Q54905425
RX   CelloPub=CLPUB00751;
RX   DOI=10.1007/BF02948354;
RX   PubMed=24743803;
RX   PubMed=28107433;
RX   PubMed=28851942;
RX   PubMed=29683800;
RX   PubMed=29717028;
RX   PubMed=37543987;
RX   PubMed=38079103;
WW   https://iclac.org/wp-content/uploads/Cross-Contaminations_v13_distribution.xlsx
CC   Problematic cell line: Contaminated. Seems to be an hybrid of HeLa and probably of a gastric cancer cell from an Asian individual. The STR profile is highly similar to that of HeLa yet shows some specific differences that suggest it is an hybrid cell line (PubMed=37543987; PubMed=38079103). Was originally thought to originate from a 53 year old male patient suffering from a poorly differentiated gastric mucinous adenocarcinoma.
CC   Registration: International Cell Line Authentication Committee, Register of Misidentified Cell Lines; ICLAC-00588.
CC   Doubling time: ~25 hours (CelloPub=CLPUB00751); 22.5 hours (PubMed=29683800).
CC   Transformant: NCBI_TaxID; 333761; Human papillomavirus type 18 (HPV18).
CC   Omics: Secretome proteome analysis by 2D-DE/MS.
CC   Omics: SNP array analysis.
CC   Omics: Transcriptome analysis by microarray.
CC   Omics: Transcriptome analysis by RNAseq.
CC   Miscellaneous: Formerly the CCRID database had 2 entries describing this cell line (3111C0001CCC000227, 3131C0001000700084). They were one of the sources for the STR profile of this entry.
CC   Misspelling: BGC-803; Note=Occasionally.
CC   Misspelling: BGC803; Note=Occasionally.
CC   Misspelling: MCG803; GEO=GSM844588.
ST   Source(s): CCRID; PubMed=28107433; PubMed=28851942; PubMed=37543987; PubMed=38079103
ST   Amelogenin: X
ST   CSF1PO: 9,10,12
ST   D10S1248: 15
ST   D12S391: 17,20,26
ST   D13S317: 7,13.3
ST   D16S539: 9,11
ST   D18S51: 13
ST   D19S433: 13
ST   D1S1656: 12,15,16
ST   D21S11: 27,28
ST   D22S1045: 15,17
ST   D2S1338: 17,24
ST   D2S441: 10,11
ST   D3S1358: 15,18
ST   D5S818: 10,11,12
ST   D6S1043: 18,19
ST   D7S820: 11,12
ST   D8S1179: 12,14
ST   FGA: 18,21
ST   Penta D: 8,15
ST   Penta E: 7,10,16,17
ST   TH01: 7,9
ST   TPOX: 12
ST   vWA: 16,17,18
OX   NCBI_TaxID=9606; ! Homo sapiens (Human)
HI   CVCL_0030 ! HeLa
CA   Hybrid cell line
DT   Created: 04-04-12; Last updated: 02-05-24; Version: 29
//
RX   CelloPub=CLPUB00751;
RA   Wang K.-H.;
RT   "A in vitro cell (MGc80-3) of a poorly differentiated mucoid
RT   adenocarcinoma of human stomach.";
RL   Shi Yan Sheng Wu Xue Bao 16:257-266(1983).
//
RX   DOI=10.1007/BF02948354;
RA   Xiao H., Dong Z.-W., Shou C.-C.;
RT   "Induction of apoptosis in human gastric carcinoma cell line MGC-803
RT   by monoclonal antibody PD4.";
RL   Chin. J. Cancer Res. 10:169-173(1998).
//
RX   PubMed=24743803;
RA   Huang H., Han Y., Wu J., Tian Z.-H., Qu L.-K., Shou C.-C.;
RT   "Establishment of drug resistant cell line of MGC-803 and analysis of
RT   differential secretome.";
RL   Beijing Da Xue Xue Bao Yi Xue Ban 46:183-189(2014).
//
RX   PubMed=28107433; DOI=10.1371/journal.pone.0170384;
RA   Huang Y.-Q., Liu Y.-H., Zheng C.-Y., Shen C.;
RT   "Investigation of cross-contamination and misidentification of 278
RT   widely used tumor cell lines.";
RL   PLoS ONE 12:E0170384-E0170384(2017).
//
RX   PubMed=28851942; DOI=10.1038/s41598-017-09660-w;
RA   Bian X.-C., Yang Z.-L., Feng H.-L., Sun H., Liu Y.-Q.;
RT   "A combination of species identification and STR profiling identifies
RT   cross-contaminated cells from 482 human tumor cell lines.";
RL   Sci. Rep. 7:9774-9774(2017).
//
RX   PubMed=29683800; DOI=10.1097/CAD.0000000000000601;
RA   Fu L., Yin F., Li X.-R., Han B.-K., Zhang C., Wang J.-W., Wang Y.-Q.,
RA   Bi Y.-F., Liu H.-M.;
RT   "Generation and characterization of a paclitaxel-resistant human
RT   gastric carcinoma cell line.";
RL   Anticancer Drugs 29:491-502(2018).
//
RX   PubMed=29717028; DOI=10.1042/BSR20180277;
RA   Zhang Y.-Q., Yu S.-T., Zhang Z.-Z., Zhao G., Xu J.;
RT   "Long non-coding RNA AK096174 promotes cell proliferation and invasion
RT   in gastric cancer by regulating WDR66 expression.";
RL   Biosci. Rep. 38:BSR20180277.1-BSR20180277.12(2018).
//
RX   PubMed=37543987; DOI=10.1002/ijc.34677;
RA   Cao F., Sun H., Yang Z.-L., Bai Y.-H., Hu X., Hou Y.-H., Bian X.-C.,
RA   Liu Y.-Q.;
RT   "Multiple approaches revealed MGc80-3 as a somatic hybrid with HeLa
RT   cells rather than a gastric cancer cell line.";
RL   Int. J. Cancer 154:155-168(2023).
//
RX   PubMed=38079103; DOI=10.1007/s13577-023-01011-4;
RA   Yang M.-M., He J., Xia S.-X., Wang Y.-D., Xiong J., Liao C., Li N.,
RA   Qu S.-F., Shen C.;
RT   "Investigation of the mixed origins of the MGC-803 cell line reveals
RT   that it is a hybrid cell line derived from HeLa.";
RL   Hum. Cell 37:560-566(2024).
//