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Cellosaurus U-CH2 (CVCL_4989)

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Cell line name U-CH2
Synonyms UCH-2; UCH2
Accession CVCL_4989
Resource Identification Initiative To cite this cell line use: U-CH2 (RRID:CVCL_4989)
Comments Part of: Cancer Dependency Map project (DepMap) (includes Cancer Cell Line Encyclopedia - CCLE).
Part of: COSMIC cell lines project.
Population: Caucasian.
Doubling time: 4 days (PubMed=26183925); ~5-7 days (DSMZ=ACC-749); 3 days (Chordoma Foundation).
Microsatellite instability: Stable (MSS) (Sanger).
Omics: CNV analysis.
Omics: Deep exome analysis.
Omics: Deep quantitative proteome analysis.
Omics: DNA methylation analysis.
Omics: Genome sequenced.
Omics: Transcriptome analysis by microarray.
Omics: Transcriptome analysis by RNAseq.
HLA typing Source: Chordoma Foundation
Class I
HLA-AA*11:01
HLA-BB*07
HLA-CC*07:02
Class II
HLA-DQDQA1*02:01
DQB1*03
HLA-DRDRB4*01:03N
Genome ancestry Source: PubMed=30894373

Origin% genome
African0
Native American0.92
East Asian, North0
East Asian, South0.34
South Asian1.36
European, North67.93
European, South29.44
Disease Sacral chordoma (NCIt: C129927)
Chordoma (ORDO: Orphanet_178)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Sex of cell Female
Age at sampling 72Y
Category Cancer cell line
STR profile Source(s): ATCC=CRL-3218; Cosmic-CLP=1503373; DSMZ=ACC-749; PubMed=21253487; PubMed=26183925; PubMed=27102572; PubMed=28835717

Markers:
AmelogeninX
CSF1PO11,12
D1S165614.3,15
D2S44110,11.3
D2S133824
D3S135817
D5S81810,11
D7S8208,12
D8S117913
D10S124813,16
D12S39118,19
D13S31711
D16S53912
D18S5112,18 (PubMed=26183925; PubMed=27102572; PubMed=28835717)
12,17.3 (DSMZ=ACC-749)
D19S43315.2,17 (PubMed=26183925; PubMed=27102572)
16,17 (DSMZ=ACC-749)
D21S1129,30
D22S104515
FGA21,22.2
Penta D12,13
Penta E12,15
TH016 (Cosmic-CLP=1503373)
6,9.3 (PubMed=21253487)
9.3 (ATCC=CRL-3218; DSMZ=ACC-749; PubMed=26183925; PubMed=27102572)
TPOX8
vWA17

Run an STR similarity search on this cell line
Web pages https://www.chordomafoundation.org/researchers/disease-models/u-ch2/
http://genome.ucsc.edu/ENCODE/protocols/cell/human/UCH1_Crawford_protocol.pdf
Publications

PubMed=21253487; DOI=10.1155/2010/630129; PMCID=PMC3022207
Bruderlein S., Sommer J.B., Meltzer P.S., Li S.-F., Osada T., Ng D., Moller P., Alcorta D.A., Kelley M.J.
Molecular characterization of putative chordoma cell lines.
Sarcoma 2010:630129.1-630129.14(2010)

PubMed=26183925; DOI=10.1158/0008-5472.CAN-14-3270
von Witzleben A., Goerttler L.T., Marienfeld R., Barth H., Lechel A., Mellert K., Bohm M., Kornmann M., Mayer-Steinacker R., von Baer A., Schultheiss M., Flanagan A.M., Moller P., Bruderlein S., Barth T.F.E.
Preclinical characterization of novel chordoma cell systems and their targeting by pharmocological inhibitors of the CDK4/6 cell-cycle pathway.
Cancer Res. 75:3823-3831(2015)

PubMed=27102572; DOI=10.1002/path.4729; PMCID=PMC4922416
Scheipl S., Barnard M., Cottone L., Jorgensen M., Drewry D.H., Zuercher W.J., Turlais F., Ye H.-T., Leite A.P., Smith J.A., Leithner A., Moller P., Bruderlein S., Guppy N., Amary F., Tirabosco R., Strauss S.J., Pillay N., Flanagan A.M.
EGFR inhibitors identified as a potential treatment for chordoma in a focused compound screen.
J. Pathol. 239:320-334(2016)

PubMed=27397505; DOI=10.1016/j.cell.2016.06.017; PMCID=PMC4967469
Iorio F., Knijnenburg T.A., Vis D.J., Bignell G.R., Menden M.P., Schubert M., Aben N., Goncalves E., Barthorpe S., Lightfoot H., Cokelaer T., Greninger P., van Dyk E., Chang H., de Silva H., Heyn H., Deng X.-M., Egan R.K., Liu Q.-S., Mironenko T., Mitropoulos X., Richardson L., Wang J.-H., Zhang T.-H., Moran S., Sayols S., Soleimani M., Tamborero D., Lopez-Bigas N., Ross-Macdonald P., Esteller M., Gray N.S., Haber D.A., Stratton M.R., Benes C.H., Wessels L.F.A., Saez-Rodriguez J., McDermott U., Garnett M.J.
A landscape of pharmacogenomic interactions in cancer.
Cell 166:740-754(2016)

PubMed=28515451; DOI=10.1038/s41598-017-02174-5; PMCID=PMC5435709
Jager D., Barth T.F.E., Bruderlein S., Scheuerle A., Rinner B., von Witzleben A., Lechel A., Meyer P., Mayer-Steinacker R., von Baer A., Schultheiss M., Wirtz C.R., Moller P., Mellert K.
HOXA7, HOXA9, and HOXA10 are differentially expressed in clival and sacral chordomas.
Sci. Rep. 7:2032-2032(2017)

PubMed=28835717; DOI=10.1038/s41598-017-10044-3; PMCID=PMC5569021
Bosotti R., Magnaghi P., Di Bella S., Cozzi L., Cusi C., Bozzi F., Beltrami N., Carapezza G., Ballinari D., Amboldi N., Lupi R., Somaschini A., Raddrizzani L., Salom B., Galvani A., Stacchiotti S., Tamborini E., Isacchi A.
Establishment and genomic characterization of the new chordoma cell line Chor-IN-1.
Sci. Rep. 7:9226-9226(2017)

PubMed=30894373; DOI=10.1158/0008-5472.CAN-18-2747; PMCID=PMC6445675
Dutil J., Chen Z.-H., Monteiro A.N.A., Teer J.K., Eschrich S.A.
An interactive resource to probe genetic diversity and estimated ancestry in cancer cell lines.
Cancer Res. 79:1263-1273(2019)

PubMed=35839778; DOI=10.1016/j.ccell.2022.06.010; PMCID=PMC9387775
Goncalves E., Poulos R.C., Cai Z.-X., Barthorpe S., Manda S.S., Lucas N., Beck A., Bucio-Noble D., Dausmann M., Hall C., Hecker M., Koh J., Lightfoot H., Mahboob S., Mali I., Morris J., Richardson L., Seneviratne A.J., Shepherd R., Sykes E., Thomas F., Valentini S., Williams S.G., Wu Y.-X., Xavier D., MacKenzie K.L., Hains P.G., Tully B., Robinson P.J., Zhong Q., Garnett M.J., Reddel R.R.
Pan-cancer proteomic map of 949 human cell lines.
Cancer Cell 40:835-849.e8(2022)

Cross-references
Cell line collections (Providers) ATCC; CRL-3218
DSMZ; ACC-749
Cell line databases/resources Cell_Model_Passport; SIDM01185
Cosmic-CLP; 1503373
DepMap; ACH-002204
DSMZCellDive; ACC-749
Chemistry resources PharmacoDB; UCH2_1627_2019
Encyclopedic resources Wikidata; Q54973617
Gene expression databases GEO; GSM1670565
GEO; GSM1673847
GEO; GSM1673848
Polymorphism and mutation databases Cosmic; 2663858
Proteomic databases PRIDE; PXD030304
Entry history
Entry creation04-Apr-2012
Last entry update02-May-2024
Version number29