ID   pgsD-677
AC   CVCL_4593
SY   PgsD-677 pgsD677; 677; Mutant 677; CHO-677; CHO 677
DR   CLO; CLO_0008438
DR   ATCC; CRL-2244
DR   Wikidata; Q54947334
RX   PubMed=1532254;
CC   Characteristics: Lacks both N-acetylglucosaminyltransferase and glucuronyltransferase activities that are required for synthesis of heparan sulfate.
CC   Transformant: ChEBI; CHEBI:23994; Ethyl methanesulfonate (Methylsulfonic acid ethyl ester; MSEE).
CC   Derived from site: In situ; Ovary; UBERON=UBERON_0000992.
CC   Cell type: Epithelial cell of ovary; CL=CL_2000064.
OX   NCBI_TaxID=10029; ! Cricetulus griseus (Chinese hamster)
HI   CVCL_0214 ! CHO-K1
SX   Female
AG   Adult
CA   Spontaneously immortalized cell line
DT   Created: 04-04-12; Last updated: 30-01-24; Version: 10
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RX   PubMed=1532254; DOI=10.1073/pnas.89.6.2267;
RA   Lidholt K., Weinke J.L., Kiser C.S., Lugemwa F.N., Bame K.J.,
RA   Cheifetz S., Massague J., Lindahl U., Esko J.D.;
RT   "A single mutation affects both N-acetylglucosaminyltransferase and
RT   glucuronosyltransferase activities in a Chinese hamster ovary cell
RT   mutant defective in heparan sulfate biosynthesis.";
RL   Proc. Natl. Acad. Sci. U.S.A. 89:2267-2271(1992).
//