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Cellosaurus MCF-12A (CVCL_3744)

[Text version]
Cell line name MCF-12A
Synonyms MCF12A; Michigan Cancer Foundation-12A
Accession CVCL_3744
Resource Identification Initiative To cite this cell line use: MCF-12A (RRID:CVCL_3744)
Comments Group: Patented cell line.
Part of: Cancer Dependency Map project (DepMap) (includes Cancer Cell Line Encyclopedia - CCLE).
Part of: JWGray breast cancer cell line panel.
Part of: ICBP43 breast cancer cell line panel.
Registration: International Depositary Authority, American Type Culture Collection (ATCC); CRL-10782.
Population: Caucasian.
Doubling time: ~19 hours (ATCC=CRL-3598); 35.42 hours (JWGray panel).
Omics: Deep exome analysis.
Omics: DNA methylation analysis.
Omics: Glycoproteome analysis by proteomics.
Omics: Protein expression by reverse-phase protein arrays.
Omics: SNP array analysis.
Omics: Transcriptome analysis by microarray.
Omics: Transcriptome analysis by RNAseq.
Derived from site: In situ; Breast, epithelium; UBERON=UBERON_0008367.
HLA typing Source: PubMed=25960936
Class I
HLA-AA*66:01,02:01
HLA-BB*41:02,18:01/35:08
HLA-CC*07:01,17:01
Class II
HLA-DRDRB1*11:67,11:67
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Originate from same individual CVCL_3745 ! MCF-12F
Sex of cell Female
Age at sampling 63Y
Category Spontaneously immortalized cell line
STR profile Source(s): ATCC=CRL-3598

Markers:
AmelogeninX
CSF1PO10,11
D2S133819
D3S135816,18
D5S81811,13
D7S8208,11
D8S117910,13
D13S3179,11
D16S5399,12
D18S5118
D19S43313,15
D21S1128,30
FGA23,26
Penta D9,12
Penta E7,10
TH017
TPOX8
vWA18

Run an STR similarity search on this cell line
Web pages https://www.atcc.org/en/support/technical-support/faqs/normal-morphology-of-the-crl-10782-mcf-12a-cells
https://lincs.hms.harvard.edu/resources/reagents/icbp43/
https://www.synapse.org/#!Synapse:syn2346643/wiki/62255
Publications

Patent=US5206165
Pauley R.J., Paine T.J., Soule H.D.
Immortal human mammary epithelial cell sublines.
Patent number US5206165, 27-Apr-1993

PubMed=17157791; DOI=10.1016/j.ccr.2006.10.008; PMCID=PMC2730521
Neve R.M., Chin K., Fridlyand J., Yeh J., Baehner F.L., Fevr T., Clark L., Bayani N., Coppe J.-P., Tong F., Speed T., Spellman P.T., DeVries S., Lapuk A., Wang N.J., Kuo W.-L., Stilwell J.L., Pinkel D., Albertson D.G., Waldman F.M., McCormick F., Dickson R.B., Johnson M.D., Lippman M.E., Ethier S.P., Gazdar A.F., Gray J.W.
A collection of breast cancer cell lines for the study of functionally distinct cancer subtypes.
Cancer Cell 10:515-527(2006)

PubMed=18516279; DOI=10.1016/j.molonc.2007.02.004; PMCID=PMC2391005
Kenny P.A., Lee G.Y., Myers C.A., Neve R.M., Semeiks J.R., Spellman P.T., Lorenz K., Lee E.H., Barcellos-Hoff M.H., Petersen O.W., Gray J.W., Bissell M.J.
The morphologies of breast cancer cell lines in three-dimensional assays correlate with their profiles of gene expression.
Mol. Oncol. 1:84-96(2007)

PubMed=24094812; DOI=10.1016/j.ccr.2013.08.020; PMCID=PMC3931310
Timmerman L.A., Holton T., Yuneva M., Louie R.J., Padro M., Daemen A., Hu M., Chan D.A., Ethier S.P., van 't Veer L.J., Polyak K., McCormick F., Gray J.W.
Glutamine sensitivity analysis identifies the xCT antiporter as a common triple-negative breast tumor therapeutic target.
Cancer Cell 24:450-465(2013)

PubMed=24162158; DOI=10.1007/s10549-013-2743-3; PMCID=PMC3832776
Prat A., Karginova O., Parker J.S., Fan C., He X.-P., Bixby L.M., Harrell J.C., Roman E., Adamo B., Troester M.A., Perou C.M.
Characterization of cell lines derived from breast cancers and normal mammary tissues for the study of the intrinsic molecular subtypes.
Breast Cancer Res. Treat. 142:237-255(2013)

PubMed=24176112; DOI=10.1186/gb-2013-14-10-r110; PMCID=PMC3937590
Daemen A., Griffith O.L., Heiser L.M., Wang N.J., Enache O.M., Sanborn Z., Pepin F., Durinck S., Korkola J.E., Griffith M., Hur J.S., Huh N., Chung J., Cope L., Fackler M.J., Umbricht C.B., Sukumar S., Seth P., Sukhatme V.P., Jakkula L.R., Lu Y.-L., Mills G.B., Cho R.J., Collisson E.A., van 't Veer L.J., Spellman P.T., Gray J.W.
Modeling precision treatment of breast cancer.
Genome Biol. 14:R110.1-R110.14(2013)

PubMed=25960936; DOI=10.4161/21624011.2014.954893; PMCID=PMC4355981
Boegel S., Lower M., Bukur T., Sahin U., Castle J.C.
A catalog of HLA type, HLA expression, and neo-epitope candidates in human cancer cell lines.
OncoImmunology 3:e954893.1-e954893.12(2014)

PubMed=28287265; DOI=10.1021/acs.jproteome.6b00470; PMCID=PMC5557415
Yen T.-Y., Bowen S., Yen R., Piryatinska A., Macher B.A., Timpe L.C.
Glycoproteins in claudin-low breast cancer cell lines have a unique expression profile.
J. Proteome Res. 16:1391-1400(2017)

Cross-references
Cell line collections (Providers) ATCC; CRL-3598
ATCC; CRL-10782 - Discontinued
Cell line databases/resources CLO; CLO_0007603
CLO; CLO_0037180
DepMap; ACH-002327
LINCS_HMS; 51089
LINCS_LDP; LCL-2082
TOKU-E; 2381
Anatomy/cell type resources BTO; BTO:0002544
Biological sample resources BioSample; SAMN03472816
Chemistry resources ChEMBL-Cells; CHEMBL3307365
ChEMBL-Targets; CHEMBL614322
PharmacoDB; MCF12A_894_2019
PubChem_Cell_line; CVCL_3744
Encyclopedic resources Wikidata; Q54904301
Experimental variables resources EFO; EFO_0001202
Gene expression databases ArrayExpress; E-TABM-157
ArrayExpress; E-TABM-244
GEO; GSM756372
GEO; GSM845396
GEO; GSM844585
GEO; GSM1053717
GEO; GSM1172975
GEO; GSM1172884
GEO; GSM1238117
Polymorphism and mutation databases Cosmic; 1017163
Cosmic; 1136378
Cosmic; 1176637
Cosmic; 2560249
Proteomic databases PRIDE; PXD005339
Entry history
Entry creation04-Apr-2012
Last entry update10-Sep-2024
Version number31