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Cellosaurus EBC-1 (CVCL_2891)

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Cell line name EBC-1
Synonyms EBC-1/original; EBC1
Accession CVCL_2891
Resource Identification Initiative To cite this cell line use: EBC-1 (RRID:CVCL_2891)
Comments Part of: Cancer Dependency Map project (DepMap) (includes Cancer Cell Line Encyclopedia - CCLE).
Part of: COSMIC cell lines project.
Population: Chinese; Taiwan.
Doubling time: 42 hours (DOI=10.4044/joma1947.97.7-8_691); 38.7 hours (PubMed=8286010).
Microsatellite instability: Stable (MSS) (Sanger).
Omics: CRISPR phenotypic screen.
Omics: Deep exome analysis.
Omics: Deep quantitative proteome analysis.
Omics: DNA methylation analysis.
Omics: SNP array analysis.
Omics: Transcriptome analysis by microarray.
Omics: Transcriptome analysis by RNAseq.
Derived from site: Metastatic; Skin; UBERON=UBERON_0002097.
Sequence variations
  • Mutation; HGNC; 2731; DDR2; Simple; p.Thr681Ile (c.2042C>T); ClinVar=VCV001435412; Zygosity=Heterozygous (PubMed=28676216; Cosmic-CLP=753554).
  • Mutation; HGNC; 3236; EGFR; Simple; p.Leu858Arg (c.2573T>G); ClinVar=VCV000016609; Zygosity=Heterozygous; Note=In 10% of cells (PubMed=16105816).
  • Mutation; HGNC; 11998; TP53; Simple; p.Glu171Ter (c.511G>T); ClinVar=VCV000634771; Zygosity=Homozygous (PubMed=10536175; PubMed=20557307; Cosmic-CLP=753554; DepMap=ACH-000563).
HLA typing Source: PubMed=26589293
Class I
HLA-AA*24:02,24:02
HLA-BB*07:02,07:02
HLA-CC*07:02,07:02
Class II
HLA-DQDQA1*01:01,01:01
DQB1*05:01,05:01
HLA-DRDRB1*01:01,15:01
Genome ancestry Source: PubMed=30894373

Origin% genome
African1.01
Native American0
East Asian, North85.35
East Asian, South10.91
South Asian0
European, North0
European, South2.73
Disease Lung squamous cell carcinoma (NCIt: C3493)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Children:
CVCL_LC83 (EBC-1 KRAS (G12D/+/+/+))CVCL_U892 (EBC-1/bone)CVCL_U893 (EBC-1/brain)
Sex of cell Male
Age at sampling 69Y
Category Cancer cell line
STR profile Source(s): Cosmic-CLP=753554; JCRB=JCRB0820; PubMed=25877200; RCB=RCB1965; TKG=TKG 0481

Markers:
AmelogeninX
CSF1PO10
D3S135815
D5S81811
D7S82010,11
D8S117914
D13S31712
D16S5398,9 (RCB=RCB1965)
9 (Cosmic-CLP=753554; JCRB=JCRB0820; PubMed=25877200; TKG=TKG 0481)
D18S5112,17
D21S1129,30
FGA22
Penta D13
Penta E16
TH017
TPOX8
vWA16,17

Run an STR similarity search on this cell line
Web pages https://www.thermofisher.com/ch/en/home/technical-resources/cell-lines/e/cell-lines-detail-570.html
Publications

DOI=10.2482/haigan.22.53
Hiraki S., Miyai M., Seto T., Tamura T., Watanabe Y., Ozawa S., Ikeda H., Nakata Y., Ohnoshi T., Kimura I.
Establishment of human continuous cell lines from squamous cell, adeno- and small cell carcinoma of the lung and the results of heterotransplantation.
Nihon Haigan Gakkai 22:53-58(1982)

DOI=10.4044/joma1947.97.7-8_691
Watanabe Y.
Experimental model of human lung cancer, Part I. Establishment and characterization of new tissue culture cell lines from human squamous cell carcinoma and adenocarcinoma of the lung.
Okayama Igakkai Zasshi 97:691-700(1985)

DOI=10.4044/joma1947.97.7-8_701
Watanabe Y.
Experimental model of human lung cancer, Part II Heterotransplantation of human continuous cell lines from squamous cell carcinoma and adenocarcinoma of the lung.
Okayama Igakkai Zasshi 97:701-712(1985)

PubMed=8286010
Kiura K., Watarai S., Shibayama T., Ohnoshi T., Kimura I., Yasuda T.
Inhibitory effects of cholera toxin on in vitro growth of human lung cancer cell lines.
Anticancer Drug Des. 8:417-428(1993)

PubMed=9290701; DOI=10.1002/(SICI)1098-2744(199708)19:4<243::AID-MC5>3.0.CO;2-D
Jia L.-Q., Osada M., Ishioka C., Gamo M., Ikawa S., Suzuki T., Shimodaira H., Niitani T., Kudo T., Akiyama M., Kimura N., Matsuo M., Mizusawa H., Tanaka N., Koyama H., Namba M., Kanamaru R., Kuroki T.
Screening the p53 status of human cell lines using a yeast functional assay.
Mol. Carcinog. 19:243-253(1997)

PubMed=10358721; DOI=10.18926/AMO/31626
Matsushita A., Tabata M., Ueoka H., Kiura K., Shibayama T., Aoe K., Kohara H., Harada M.
Establishment of a drug sensitivity panel using human lung cancer cell lines.
Acta Med. Okayama 53:67-75(1999)

PubMed=10536175; DOI=10.3892/ijo.15.5.927
Fujita T., Kiyama M., Tomizawa Y., Kohno T., Yokota J.
Comprehensive analysis of p53 gene mutation characteristics in lung carcinoma with special reference to histological subtypes.
Int. J. Oncol. 15:927-934(1999)

PubMed=11005564; DOI=10.1038/sj.neo.7900094; PMCID=PMC1550293
Kohno T., Sato T., Takakura S., Takei K., Inoue K., Nishioka M., Yokota J.
Mutation and expression of the DCC gene in human lung cancer.
Neoplasia 2:300-305(2000)

PubMed=12569360; DOI=10.1038/sj.onc.1206106
Funakoshi T., Tachibana I., Hoshida Y., Kimura H., Takeda Y., Kijima T., Nishino K., Goto H., Yoneda T., Kumagai T., Osaki T., Hayashi S., Aozasa K., Kawase I.
Expression of tetraspanins in human lung cancer cells: frequent downregulation of CD9 and its contribution to cell motility in small cell lung cancer.
Oncogene 22:674-687(2003)

PubMed=14581340
Yokoi S., Yasui K., Iizasa T., Imoto I., Fujisawa T., Inazawa J.
TERC identified as a probable target within the 3q26 amplicon that is detected frequently in non-small cell lung cancers.
Clin. Cancer Res. 9:4705-4713(2003)

PubMed=15185009; DOI=10.1007/s00262-004-0533-9; PMCID=PMC11034254
Yamaji H., Iizasa T., Koh E., Suzuki M., Otsuji M., Chang H., Motohashi S., Yokoi S., Hiroshima K., Tagawa M., Nakayama T., Fujisawa T.
Correlation between interleukin 6 production and tumor proliferation in non-small cell lung cancer.
Cancer Immunol. Immunother. 53:786-792(2004)

PubMed=15746151; DOI=10.1093/hmg/ddi092
Izumi H., Inoue J., Yokoi S., Hosoda H., Shibata T., Sunamori M., Hirohashi S., Inazawa J., Imoto I.
Frequent silencing of DBC1 is by genetic or epigenetic mechanisms in non-small cell lung cancers.
Hum. Mol. Genet. 14:997-1007(2005)

PubMed=16105816; DOI=10.1158/0008-5472.CAN-05-0331
Nagai Y., Miyazawa H., Hu Q., Tanaka T., Udagawa K., Kato M., Fukuyama S., Yokote A., Kobayashi K., Kanazawa M., Hagiwara K.
Genetic heterogeneity of the epidermal growth factor receptor in non-small cell lung cancer cell lines revealed by a rapid and sensitive detection system, the peptide nucleic acid-locked nucleic acid PCR clamp.
Cancer Res. 65:7276-7282(2005)

PubMed=17325662; DOI=10.1038/sj.onc.1210330
Nakaya K., Yamagata H.D., Arita N., Nakashiro K.-i., Nose M., Miki T., Hamakawa H.
Identification of homozygous deletions of tumor suppressor gene FAT in oral cancer using CGH-array.
Oncogene 26:5300-5308(2007)

PubMed=19472407; DOI=10.1002/humu.21028; PMCID=PMC2900846
Blanco R., Iwakawa R., Tang M.-Y., Kohno T., Angulo B., Pio R., Montuenga L.M., Minna J.D., Yokota J., Sanchez-Cespedes M.
A gene-alteration profile of human lung cancer cell lines.
Hum. Mutat. 30:1199-1206(2009)

PubMed=20215515; DOI=10.1158/0008-5472.CAN-09-3458; PMCID=PMC2881662
Rothenberg S.M., Mohapatra G., Rivera M.N., Winokur D., Greninger P., Nitta M., Sadow P.M., Sooriyakumar G., Brannigan B.W., Ulman M.J., Perera R.M., Wang R., Tam A., Ma X.-J., Erlander M., Sgroi D.C., Rocco J.W., Lingen M.W., Cohen E.E.W., Louis D.N., Settleman J., Haber D.A.
A genome-wide screen for microdeletions reveals disruption of polarity complex genes in diverse human cancers.
Cancer Res. 70:2158-2164(2010)

PubMed=20557307; DOI=10.1111/j.1349-7006.2010.01622.x; PMCID=PMC11158680
Iwakawa R., Kohno T., Enari M., Kiyono T., Yokota J.
Prevalence of human papillomavirus 16/18/33 infection and p53 mutation in lung adenocarcinoma.
Cancer Sci. 101:1891-1896(2010)

PubMed=22313637; DOI=10.4161/cbt.19238
Takata M., Chikumi H., Miyake N., Adachi K., Kanamori Y., Yamasaki A., Igishi T., Burioka N., Nanba E., Shimizu E.
Lack of AKT activation in lung cancer cells with EGFR mutation is a novel marker of cetuximab sensitivity.
Cancer Biol. Ther. 13:369-378(2012)

PubMed=22460905; DOI=10.1038/nature11003; PMCID=PMC3320027
Barretina J.G., Caponigro G., Stransky N., Venkatesan K., Margolin A.A., Kim S., Wilson C.J., Lehar J., Kryukov G.V., Sonkin D., Reddy A., Liu M., Murray L., Berger M.F., Monahan J.E., Morais P., Meltzer J., Korejwa A., Jane-Valbuena J., Mapa F.A., Thibault J., Bric-Furlong E., Raman P., Shipway A., Engels I.H., Cheng J., Yu G.-Y.K., Yu J.-J., Aspesi P. Jr., de Silva M., Jagtap K., Jones M.D., Wang L., Hatton C., Palescandolo E., Gupta S., Mahan S., Sougnez C., Onofrio R.C., Liefeld T., MacConaill L.E., Winckler W., Reich M., Li N.-X., Mesirov J.P., Gabriel S.B., Getz G., Ardlie K., Chan V., Myer V.E., Weber B.L., Porter J., Warmuth M., Finan P., Harris J.L., Meyerson M.L., Golub T.R., Morrissey M.P., Sellers W.R., Schlegel R., Garraway L.A.
The Cancer Cell Line Encyclopedia enables predictive modelling of anticancer drug sensitivity.
Nature 483:603-607(2012)

PubMed=24002593; DOI=10.1038/bjc.2013.452; PMCID=PMC3776976
Wu D., Pang Y., Wilkerson M.D., Wang D., Hammerman P.S., Liu J.S.
Gene-expression data integration to squamous cell lung cancer subtypes reveals drug sensitivity.
Br. J. Cancer 109:1599-1608(2013)

PubMed=25485619; DOI=10.1038/nbt.3080
Klijn C., Durinck S., Stawiski E.W., Haverty P.M., Jiang Z.-S., Liu H.-B., Degenhardt J., Mayba O., Gnad F., Liu J.-F., Pau G., Reeder J., Cao Y., Mukhyala K., Selvaraj S.K., Yu M.-M., Zynda G.J., Brauer M.J., Wu T.D., Gentleman R.C., Manning G., Yauch R.L., Bourgon R., Stokoe D., Modrusan Z., Neve R.M., de Sauvage F.J., Settleman J., Seshagiri S., Zhang Z.-M.
A comprehensive transcriptional portrait of human cancer cell lines.
Nat. Biotechnol. 33:306-312(2015)

PubMed=25877200; DOI=10.1038/nature14397
Yu M., Selvaraj S.K., Liang-Chu M.M.Y., Aghajani S., Busse M., Yuan J., Lee G., Peale F.V., Klijn C., Bourgon R., Kaminker J.S., Neve R.M.
A resource for cell line authentication, annotation and quality control.
Nature 520:307-311(2015)

PubMed=26589293; DOI=10.1186/s13073-015-0240-5; PMCID=PMC4653878
Scholtalbers J., Boegel S., Bukur T., Byl M., Goerges S., Sorn P., Loewer M., Sahin U., Castle J.C.
TCLP: an online cancer cell line catalogue integrating HLA type, predicted neo-epitopes, virus and gene expression.
Genome Med. 7:118.1-118.7(2015)

PubMed=27397505; DOI=10.1016/j.cell.2016.06.017; PMCID=PMC4967469
Iorio F., Knijnenburg T.A., Vis D.J., Bignell G.R., Menden M.P., Schubert M., Aben N., Goncalves E., Barthorpe S., Lightfoot H., Cokelaer T., Greninger P., van Dyk E., Chang H., de Silva H., Heyn H., Deng X.-M., Egan R.K., Liu Q.-S., Mironenko T., Mitropoulos X., Richardson L., Wang J.-H., Zhang T.-H., Moran S., Sayols S., Soleimani M., Tamborero D., Lopez-Bigas N., Ross-Macdonald P., Esteller M., Gray N.S., Haber D.A., Stratton M.R., Benes C.H., Wessels L.F.A., Saez-Rodriguez J., McDermott U., Garnett M.J.
A landscape of pharmacogenomic interactions in cancer.
Cell 166:740-754(2016)

PubMed=28676216; DOI=10.1016/j.lungcan.2017.05.017
Kobayashi-Watanabe N., Sato A., Watanabe T., Abe T., Nakashima C., Sueoka E., Kimura S., Sueoka-Aragane N.
Functional analysis of discoidin domain receptor 2 mutation and expression in squamous cell lung cancer.
Lung Cancer 110:35-41(2017)

PubMed=30894373; DOI=10.1158/0008-5472.CAN-18-2747; PMCID=PMC6445675
Dutil J., Chen Z.-H., Monteiro A.N.A., Teer J.K., Eschrich S.A.
An interactive resource to probe genetic diversity and estimated ancestry in cancer cell lines.
Cancer Res. 79:1263-1273(2019)

PubMed=30971826; DOI=10.1038/s41586-019-1103-9
Behan F.M., Iorio F., Picco G., Goncalves E., Beaver C.M., Migliardi G., Santos R., Rao Y., Sassi F., Pinnelli M., Ansari R., Harper S., Jackson D.A., McRae R., Pooley R., Wilkinson P., van der Meer D.J., Dow D., Buser-Doepner C.A., Bertotti A., Trusolino L., Stronach E.A., Saez-Rodriguez J., Yusa K., Garnett M.J.
Prioritization of cancer therapeutic targets using CRISPR-Cas9 screens.
Nature 568:511-516(2019)

PubMed=31068700; DOI=10.1038/s41586-019-1186-3; PMCID=PMC6697103
Ghandi M., Huang F.W., Jane-Valbuena J., Kryukov G.V., Lo C.C., McDonald E.R. 3rd, Barretina J.G., Gelfand E.T., Bielski C.M., Li H.-X., Hu K., Andreev-Drakhlin A.Y., Kim J., Hess J.M., Haas B.J., Aguet F., Weir B.A., Rothberg M.V., Paolella B.R., Lawrence M.S., Akbani R., Lu Y.-L., Tiv H.L., Gokhale P.C., de Weck A., Mansour A.A., Oh C., Shih J., Hadi K., Rosen Y., Bistline J., Venkatesan K., Reddy A., Sonkin D., Liu M., Lehar J., Korn J.M., Porter D.A., Jones M.D., Golji J., Caponigro G., Taylor J.E., Dunning C.M., Creech A.L., Warren A.C., McFarland J.M., Zamanighomi M., Kauffmann A., Stransky N., Imielinski M., Maruvka Y.E., Cherniack A.D., Tsherniak A., Vazquez F., Jaffe J.D., Lane A.A., Weinstock D.M., Johannessen C.M., Morrissey M.P., Stegmeier F., Schlegel R., Hahn W.C., Getz G., Mills G.B., Boehm J.S., Golub T.R., Garraway L.A., Sellers W.R.
Next-generation characterization of the Cancer Cell Line Encyclopedia.
Nature 569:503-508(2019)

PubMed=31978347; DOI=10.1016/j.cell.2019.12.023; PMCID=PMC7339254
Nusinow D.P., Szpyt J., Ghandi M., Rose C.M., McDonald E.R. 3rd, Kalocsay M., Jane-Valbuena J., Gelfand E.T., Schweppe D.K., Jedrychowski M.P., Golji J., Porter D.A., Rejtar T., Wang Y.K., Kryukov G.V., Stegmeier F., Erickson B.K., Garraway L.A., Sellers W.R., Gygi S.P.
Quantitative proteomics of the Cancer Cell Line Encyclopedia.
Cell 180:387-402.e16(2020)

PubMed=35839778; DOI=10.1016/j.ccell.2022.06.010; PMCID=PMC9387775
Goncalves E., Poulos R.C., Cai Z.-X., Barthorpe S., Manda S.S., Lucas N., Beck A., Bucio-Noble D., Dausmann M., Hall C., Hecker M., Koh J., Lightfoot H., Mahboob S., Mali I., Morris J., Richardson L., Seneviratne A.J., Shepherd R., Sykes E., Thomas F., Valentini S., Williams S.G., Wu Y.-X., Xavier D., MacKenzie K.L., Hains P.G., Tully B., Robinson P.J., Zhong Q., Garnett M.J., Reddel R.R.
Pan-cancer proteomic map of 949 human cell lines.
Cancer Cell 40:835-849.e8(2022)

PubMed=38639832; DOI=10.1007/s13577-024-01055-0; PMCID=PMC11194210
Kasai F., Fukushima M., Miyagi Y., Nakamura Y.
Genetic diversity among the present Japanese population: evidence from genotyping of human cell lines established in Japan.
Hum. Cell 37:944-950(2024)

Cross-references
Cell line collections (Providers) JCRB; JCRB0820
RCB; RCB1965
TKG; TKG 0481
Cell line databases/resources CLO; CLO_0050039
cancercelllines; CVCL_2891
Cell_Model_Passport; SIDM00486
CGH-DB; 185-1
CGH-DB; 9172-4
Cosmic-CLP; 753554
DepMap; ACH-000563
LINCS_LDP; LCL-1584
Anatomy/cell type resources BTO; BTO:0003001
Biological sample resources BioSample; SAMN03472045
BioSample; SAMN03472311
BioSample; SAMN10987674
CRISP screens repositories BioGRID_ORCS_Cell_line; 861
Chemistry resources ChEMBL-Cells; CHEMBL4295447
ChEMBL-Targets; CHEMBL4296411
GDSC; 753554
PharmacoDB; EBC1_315_2019
PubChem_Cell_line; CVCL_2891
Encyclopedic resources Wikidata; Q54831756
Experimental variables resources EFO; EFO_0003115
Gene expression databases ArrayExpress; E-MTAB-2706
ArrayExpress; E-MTAB-2770
ArrayExpress; E-MTAB-3610
GEO; GSM274671
GEO; GSM827595
GEO; GSM886993
GEO; GSM888062
GEO; GSM1374471
GEO; GSM1669749
Polymorphism and mutation databases Cosmic; 753554
Cosmic; 801583
Cosmic; 844845
Cosmic; 876170
Cosmic; 889089
Cosmic; 917995
Cosmic; 932929
Cosmic; 946069
Cosmic; 1048220
Cosmic; 1219069
Cosmic; 1239905
Cosmic; 1571731
Cosmic; 1656904
Cosmic; 1694670
Cosmic; 1802303
Cosmic; 2060527
Cosmic; 2125222
Cosmic; 2546867
Cosmic; 2645028
Cosmic; 2668327
IARC_TP53; 11876
IARC_TP53; 21134
LiGeA; CCLE_474
Progenetix; CVCL_2891
Proteomic databases PRIDE; PXD030304
Sequence databases EGA; EGAS00001000610
EGA; EGAS00001000978
Entry history
Entry creation04-Apr-2012
Last entry update10-Sep-2024
Version number45