Cellosaurus logo
expasy logo

Cellosaurus HCT 8 (CVCL_2478)

[Text version]
Cell line name HCT 8
Synonyms HCT-8; HCT8
Accession CVCL_2478
Resource Identification Initiative To cite this cell line use: HCT 8 (RRID:CVCL_2478)
Comments Part of: Cancer Dependency Map project (DepMap) (includes Cancer Cell Line Encyclopedia - CCLE).
Part of: MD Anderson Cell Lines Project.
Population: Caucasian.
Virology: Not susceptible to infection by SARS coronavirus 2 (SARS-CoV-2) (COVID-19) (PubMed=33389257).
Doubling time: 27.49 hours (PubMed=25944804).
Microsatellite instability: Instable (MSI) (PubMed=10674020; PubMed=24755471; PubMed=25926053).
Omics: N-glycan profiling.
Omics: Protein expression by reverse-phase protein arrays.
Omics: SNP array analysis.
Omics: Transcriptome analysis by microarray.
Omics: Transcriptome analysis by RNAseq.
Misspelling: HCT 18; Note=Occasionally.
Derived from site: In situ; Colon; UBERON=UBERON_0001155.
Cell type: Epithelial cell of colon; CL=CL_0011108.
Sequence variations
  • Mutation; HGNC; 583; APC; Simple; p.Arg727Met (c.2180G>T); Zygosity=Unspecified (PubMed=24755471).
  • Mutation; HGNC; 583; APC; Simple; p.Lys993Asn (c.2979G>T); Zygosity=Unspecified (PubMed=24755471).
  • Mutation; HGNC; 583; APC; Simple; p.Ile1417Leufs*2 (c.4248delC); Zygosity=Unspecified (PubMed=24755471).
  • Mutation; HGNC; 583; APC; Simple; p.Arg2166Ter (c.6496C>T); ClinVar=VCV000438883; Zygosity=Unspecified (PubMed=24755471).
  • Mutation; HGNC; 2514; CTNNB1; Simple; p.Val79Ile (c.235G>A); Zygosity=Unspecified (PubMed=24755471).
  • Mutation; HGNC; 6407; KRAS; Simple; p.Gly13Asp (c.38G>A); ClinVar=VCV000012580; Zygosity=Unspecified (PubMed=20570890; PubMed=24755471).
  • Mutation; HGNC; 8975; PIK3CA; Simple; p.Glu545Lys (c.1633G>A); ClinVar=VCV000013655; Zygosity=Unspecified (PubMed=20570890; PubMed=24755471).
  • Mutation; HGNC; 8975; PIK3CA; Simple; p.Asp549Asn (c.1645G>A); ClinVar=VCV000375900; Zygosity=Unspecified (PubMed=20570890; PubMed=24755471).
  • Mutation; HGNC; 6768; SMAD2; Simple; p.Pro164fs*8 (c.490_491insA); Zygosity=Unspecified (PubMed=24755471).
  • Mutation; HGNC; 6769; SMAD3; Simple; p.Val244Ile (c.730G>A); ClinVar=VCV000919044; Zygosity=Unspecified (PubMed=24755471).
HLA typing Source: PubMed=77569
Class I
HLA-AA*03,09
HLA-BB*08

Source: PubMed=26589293
Class I
HLA-AA*02:01,24:02
HLA-BB*08:01,35:01
HLA-CC*04:01,07:01
Disease Colon adenocarcinoma (NCIt: C4349)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_3449 (__Parent_cell_line_of_DLD-1/HCT 8/HCT 15/HRT-18)
Children:
CVCL_5679 (HCT-8/E11)CVCL_5945 (HCT-8/R1)CVCL_5947 (HCT-8/S10)
CVCL_5682 (HCT-8/S11)CVCL_A9CF (HCT-8/VCR)CVCL_A9CQ (HCT-8-Luc2-tdT)
CVCL_A9CR (HCT-8-mCherry)CVCL_A9CS (HCT-8-tdT)CVCL_S851 (HCT-8R)
Sex of cell Male
Age at sampling 67Y
Category Cancer cell line
STR profile Source(s): AddexBio=C0009017/4907; CCRID; CLS=300210; KCLB=10244; PubMed=25877200; PubMed=25926053

Markers:
AmelogeninX (CCRID; PubMed=25877200)
X,Y (AddexBio=C0009017/4907; CLS=300210; KCLB=10244; PubMed=25926053)
CSF1PO12
D2S133817,25
D3S135817
D5S81813
D7S82010,11.3 (CCRID; PubMed=25877200)
10,11.3,12 (AddexBio=C0009017/4907)
10,12 (CLS=300210; KCLB=10244; PubMed=25926053)
D8S117915
D13S3178,11
D16S53912,13
D18S5111,17
D19S43314,16
D21S1129,32.2
FGA22
Penta D9,14
Penta E7,14
TH017,9.3
TPOX8,11
vWA18,19

Run an STR similarity search on this cell line
Web pages https://tcpaportal.org/mclp/
Publications

PubMed=77569; DOI=10.1111/j.1399-0039.1978.tb01259.x
Espmark J.A., Ahlqvist-Roth L., Sarne L., Persson A.
Tissue typing of cells in culture. III. HLA antigens of established human cell lines. Attempts at typing by the mixed hemadsorption technique.
Tissue Antigens 11:279-286(1978)

PubMed=8422623; DOI=10.1002/1097-0142(19930115)71:2<315::AID-CNCR2820710208>3.0.CO;2-B
Tibbetts L.M., Doremus C.M., Tzanakakis G.N., Vezeridis M.P.
Liver metastases with 10 human colon carcinoma cell lines in nude mice and association with carcinoembryonic antigen production.
Cancer 71:315-321(1993)

PubMed=9809040; DOI=10.1016/S0165-4608(98)00081-8
Vermeulen S.J., Chen T.-R., Speleman F., Nollet F., Van Roy F.M., Mareel M.M.
Did the four human cancer cell lines DLD-1, HCT-15, HCT-8, and HRT-18 originate from one and the same patient?
Cancer Genet. Cytogenet. 107:76-79(1998)

PubMed=10674020; DOI=10.1016/S0959-8049(99)00206-3
Ku J.-L., Yoon K.-A., Kim D.-Y., Park J.-G.
Mutations in hMSH6 alone are not sufficient to cause the microsatellite instability in colorectal cancer cell lines.
Eur. J. Cancer 35:1724-1729(1999)

PubMed=20215515; DOI=10.1158/0008-5472.CAN-09-3458; PMCID=PMC2881662
Rothenberg S.M., Mohapatra G., Rivera M.N., Winokur D., Greninger P., Nitta M., Sadow P.M., Sooriyakumar G., Brannigan B.W., Ulman M.J., Perera R.M., Wang R., Tam A., Ma X.-J., Erlander M., Sgroi D.C., Rocco J.W., Lingen M.W., Cohen E.E.W., Louis D.N., Settleman J., Haber D.A.
A genome-wide screen for microdeletions reveals disruption of polarity complex genes in diverse human cancers.
Cancer Res. 70:2158-2164(2010)

PubMed=20570890; DOI=10.1158/0008-5472.CAN-10-0192; PMCID=PMC2943514
Janakiraman M., Vakiani E., Zeng Z.-S., Pratilas C.A., Taylor B.S., Chitale D., Halilovic E., Wilson M., Huberman K., Ricarte Filho J.C.M., Persaud Y., Levine D.A., Fagin J.A., Jhanwar S.C., Mariadason J.M., Lash A., Ladanyi M., Saltz L.B., Heguy A., Paty P.B., Solit D.B.
Genomic and biological characterization of exon 4 KRAS mutations in human cancer.
Cancer Res. 70:5901-5911(2010)

PubMed=24755471; DOI=10.1158/0008-5472.CAN-14-0013
Mouradov D., Sloggett C., Jorissen R.N., Love C.G., Li S., Burgess A.W., Arango D., Strausberg R.L., Buchanan D., Wormald S., O'Connor L., Wilding J.L., Bicknell D.C., Tomlinson I.P.M., Bodmer W.F., Mariadason J.M., Sieber O.M.
Colorectal cancer cell lines are representative models of the main molecular subtypes of primary cancer.
Cancer Res. 74:3238-3247(2014)

PubMed=25485619; DOI=10.1038/nbt.3080
Klijn C., Durinck S., Stawiski E.W., Haverty P.M., Jiang Z.-S., Liu H.-B., Degenhardt J., Mayba O., Gnad F., Liu J.-F., Pau G., Reeder J., Cao Y., Mukhyala K., Selvaraj S.K., Yu M.-M., Zynda G.J., Brauer M.J., Wu T.D., Gentleman R.C., Manning G., Yauch R.L., Bourgon R., Stokoe D., Modrusan Z., Neve R.M., de Sauvage F.J., Settleman J., Seshagiri S., Zhang Z.-M.
A comprehensive transcriptional portrait of human cancer cell lines.
Nat. Biotechnol. 33:306-312(2015)

PubMed=25877200; DOI=10.1038/nature14397
Yu M., Selvaraj S.K., Liang-Chu M.M.Y., Aghajani S., Busse M., Yuan J., Lee G., Peale F.V., Klijn C., Bourgon R., Kaminker J.S., Neve R.M.
A resource for cell line authentication, annotation and quality control.
Nature 520:307-311(2015)

PubMed=25926053; DOI=10.1038/ncomms8002
Medico E., Russo M., Picco G., Cancelliere C., Valtorta E., Corti G., Buscarino M., Isella C., Lamba S., Martinoglio B., Veronese S., Siena S., Sartore-Bianchi A., Beccuti M., Mottolese M., Linnebacher M., Cordero F., Di Nicolantonio F., Bardelli A.
The molecular landscape of colorectal cancer cell lines unveils clinically actionable kinase targets.
Nat. Commun. 6:7002.1-7002.10(2015)

PubMed=25944804; DOI=10.1158/1078-0432.CCR-14-2457
Bazzocco S., Dopeso H., Carton-Garcia F., Macaya I., Andretta E., Chionh F., Rodrigues P., Garrido M., Alazzouzi H., Nieto R., Sanchez A., Schwartz S. Jr., Bilic J., Mariadason J.M., Arango D.
Highly expressed genes in rapidly proliferating tumor cells as new targets for colorectal cancer treatment.
Clin. Cancer Res. 21:3695-3704(2015)

PubMed=26589293; DOI=10.1186/s13073-015-0240-5; PMCID=PMC4653878
Scholtalbers J., Boegel S., Bukur T., Byl M., Goerges S., Sorn P., Loewer M., Sahin U., Castle J.C.
TCLP: an online cancer cell line catalogue integrating HLA type, predicted neo-epitopes, virus and gene expression.
Genome Med. 7:118.1-118.7(2015)

PubMed=26537799; DOI=10.1074/mcp.M115.051235; PMCID=PMC4762531
Holst S., Deuss A.J.M., van Pelt G.W., van Vliet S.J., Garcia-Vallejo J.J., Koeleman C.A.M., Deelder A.M., Mesker W.E., Tollenaar R.A.E.M., Rombouts Y., Wuhrer M.
N-glycosylation profiling of colorectal cancer cell lines reveals association of fucosylation with differentiation and caudal type homebox 1 (CDX1)/villin mRNA expression.
Mol. Cell. Proteomics 15:124-140(2016)

PubMed=28196595; DOI=10.1016/j.ccell.2017.01.005; PMCID=PMC5501076
Li J., Zhao W., Akbani R., Liu W.-B., Ju Z.-L., Ling S.-Y., Vellano C.P., Roebuck P., Yu Q.-H., Eterovic A.K., Byers L.A., Davies M.A., Deng W.-L., Gopal Y.N.V., Chen G., von Euw E.M., Slamon D.J., Conklin D., Heymach J.V., Gazdar A.F., Minna J.D., Myers J.N., Lu Y.-L., Mills G.B., Liang H.
Characterization of human cancer cell lines by reverse-phase protein arrays.
Cancer Cell 31:225-239(2017)

PubMed=31068700; DOI=10.1038/s41586-019-1186-3; PMCID=PMC6697103
Ghandi M., Huang F.W., Jane-Valbuena J., Kryukov G.V., Lo C.C., McDonald E.R. 3rd, Barretina J.G., Gelfand E.T., Bielski C.M., Li H.-X., Hu K., Andreev-Drakhlin A.Y., Kim J., Hess J.M., Haas B.J., Aguet F., Weir B.A., Rothberg M.V., Paolella B.R., Lawrence M.S., Akbani R., Lu Y.-L., Tiv H.L., Gokhale P.C., de Weck A., Mansour A.A., Oh C., Shih J., Hadi K., Rosen Y., Bistline J., Venkatesan K., Reddy A., Sonkin D., Liu M., Lehar J., Korn J.M., Porter D.A., Jones M.D., Golji J., Caponigro G., Taylor J.E., Dunning C.M., Creech A.L., Warren A.C., McFarland J.M., Zamanighomi M., Kauffmann A., Stransky N., Imielinski M., Maruvka Y.E., Cherniack A.D., Tsherniak A., Vazquez F., Jaffe J.D., Lane A.A., Weinstock D.M., Johannessen C.M., Morrissey M.P., Stegmeier F., Schlegel R., Hahn W.C., Getz G., Mills G.B., Boehm J.S., Golub T.R., Garraway L.A., Sellers W.R.
Next-generation characterization of the Cancer Cell Line Encyclopedia.
Nature 569:503-508(2019)

PubMed=33389257; DOI=10.1007/s10096-020-04106-0; PMCID=PMC7778494
Wurtz N., Penant G., Jardot P., Duclos N., La Scola B.
Culture of SARS-CoV-2 in a panel of laboratory cell lines, permissivity, and differences in growth profile.
Eur. J. Clin. Microbiol. Infect. Dis. 40:477-484(2021)

Cross-references
Cell line collections (Providers) AddexBio; C0009017/4907
BCRC; 60303
CCTCC; GDC0201
CLS; 300210
ECACC; 90032006
ICLC; HTL99005
IZSLER; BS TCL 97
KCB; KCB 2011103YJ
KCLB; 10244
Cell line databases/resources CLO; CLO_0003667
CLDB; cl1577
CLDB; cl1578
CLDB; cl4949
CLDB; cl5197
cancercelllines; CVCL_2478
CCRID; 1101HUM-PUMC000104
CCRID; 3101HUMTCHu18
CCRID; 4201HUM-CCTCC00201
Cell_Model_Passport; SIDM00786
ColonAtlas; HCT8
DepMap; ACH-001084
IGRhCellID; HCT8
LINCS_LDP; LCL-1198
Anatomy/cell type resources BTO; BTO:0002255
Biological sample resources BioSample; SAMN03473138
Chemistry resources ChEMBL-Cells; CHEMBL3308035
ChEMBL-Targets; CHEMBL613510
PharmacoDB; HCT8_519_2019
PubChem_Cell_line; CVCL_2478
Encyclopedic resources Wikidata; Q54882008
Experimental variables resources EFO; EFO_0002189
Gene expression databases ArrayExpress; E-MTAB-38
ArrayExpress; E-MTAB-2706
GEO; GSM206504
GEO; GSM274709
GEO; GSM274710
GEO; GSM274723
GEO; GSM513910
GEO; GSM514294
GEO; GSM827344
GEO; GSM1346876
GEO; GSM1374523
GEO; GSM1374524
GEO; GSM1448162
Metabolomic databases MetaboLights; MTBLS227
Polymorphism and mutation databases Cosmic; 887226
Cosmic; 948853
Cosmic; 983738
Cosmic; 988873
Cosmic; 1043814
Cosmic; 1310946
Cosmic; 1479593
Cosmic; 1609484
Cosmic; 1676735
Cosmic; 2267316
Cosmic; 2301980
Cosmic; 2651867
Progenetix; CVCL_2478
Sequence databases EGA; EGAS00001000610
Entry history
Entry creation04-Apr-2012
Last entry update02-May-2024
Version number39