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Cellosaurus Ras[V12]-H3 (CVCL_1B60)

[Text version]
Cell line name Ras[V12]-H3
Synonyms Ras-H3; Ras H3; R3
Accession CVCL_1B60
Resource Identification Initiative To cite this cell line use: Ras[V12]-H3 (RRID:CVCL_1B60)
Comments Group: Insect cell line.
Doubling time: 45 hours (DGRC=200).
Genetic integration: Method=Transfection/transduction; Gene=FlyBase_Gene; FBgn0003205; Ras85D (Note=With p.Gly12Val).
Genetic integration: Method=Transfection/transduction; Gene=FPbase; 1XF1B; GFP (Note=avGFP; original A.victoria green fluorescent protein).
Omics: Transcriptome analysis by RNAseq.
Derived from site: In situ; Whole embryo; UBERON=UBERON_0000922.
Species of origin Drosophila melanogaster (Fruit fly) (NCBI Taxonomy: 7227)
Sex of cell Male
Age at sampling Embryonic stage
Category Transformed cell line
Publications

PubMed=18670627; DOI=10.1371/journal.pgen.1000142; PMCID=PMC2474701
Simcox A.A., Mitra S., Truesdell S.L., Paul L., Chen T., Butchar J.P., Justiniano S.E.
Efficient genetic method for establishing Drosophila cell lines unlocks the potential to create lines of specific genotypes.
PLoS Genet. 4:E1000142-E1000142(2008)

PubMed=26438832; DOI=10.1073/pnas.1517729112; PMCID=PMC4620889
Dequeant M.-L., Fagegaltier D., Hu Y.-H., Spirohn K., Simcox A.A., Hannon G.J., Perrimon N.
Discovery of progenitor cell signatures by time-series synexpression analysis during Drosophila embryonic cell immortalization.
Proc. Natl. Acad. Sci. U.S.A. 112:12974-12979(2015)

PubMed=26772746; DOI=10.1534/g3.115.023366; PMCID=PMC4777130
Stoiber M., Celniker S.E., Cherbas L., Brown B., Cherbas P.
Diverse hormone response networks in 41 independent Drosophila cell lines.
G3 (Bethesda) 6:683-694(2016)

Cross-references
Cell line collections (Providers) DGRC; 200
Kerafast; EF4004
Cell line databases/resources FlyBase_Cell_line; FBtc0000200
Encyclopedic resources Wikidata; Q54949221
Entry history
Entry creation08-Jul-2015
Last entry update10-Sep-2024
Version number15