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Cellosaurus ONS-76 (CVCL_1624)

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Cell line name ONS-76
Synonyms ONS76; Osaka Neurological Surgery-76
Accession CVCL_1624
Resource Identification Initiative To cite this cell line use: ONS-76 (RRID:CVCL_1624)
Comments Part of: Cancer Dependency Map project (DepMap) (includes Cancer Cell Line Encyclopedia - CCLE).
Part of: COSMIC cell lines project.
Population: Japanese.
Doubling time: 18.6 hours (CelloPub=CLPUB00094; PubMed=2504489; PubMed=2818910); 22 +- 0.3 hours (PubMed=31346954); ~24 hours (Note=Lot 08192008), ~32 hours (Note=Lot 12042014) (JCRB=IFO50355).
Microsatellite instability: Stable (MSS) (Sanger).
Omics: Deep exome analysis.
Omics: Deep quantitative proteome analysis.
Omics: DNA methylation analysis.
Omics: SNP array analysis.
Omics: Transcriptome analysis by microarray.
Omics: Transcriptome analysis by RNAseq.
Derived from site: In situ; Brain; UBERON=UBERON_0000955.
Sequence variations
  • Mutation; HGNC; 7989; NRAS; Simple; p.Gln61Arg (c.182A>G); ClinVar=VCV000013900; Zygosity=Heterozygous (Cosmic-CLP=909248; DepMap=ACH-000776).
  • Mutation; HGNC; 11730; TERT; Simple; c.1-124C>T (c.228C>T) (C228T); Zygosity=Unspecified; Note=In promoter (PubMed=31068700).
  • Mutation; HGNC; 11998; TP53; None_reported; -; Zygosity=- (Cosmic-CLP=909248; DepMap=ACH-000776).
HLA typing Source: PubMed=26589293
Class I
HLA-AA*24:02,26:03
HLA-BB*54:01,54:01
HLA-CC*01:02,03:03
Class II
HLA-DRDRB1*07:01,09:07
Genome ancestry Source: PubMed=30894373

Origin% genome
African3.13
Native American0
East Asian, North83.83
East Asian, South8.46
South Asian0
European, North0
European, South4.58
Disease Medulloblastoma, SHH-activated, TP53-wildtype (NCIt: C129443)
Medulloblastoma (ORDO: Orphanet_616)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Children:
CVCL_S734 (ONS-B11)CVCL_S735 (ONS-F11)CVCL_S736 (ONS-F8)
Sex of cell Female
Age at sampling 2Y
Category Cancer cell line
STR profile Source(s): Cosmic-CLP=909248; JCRB=IFO50355; PubMed=25877200

Markers:
AmelogeninX
CSF1PO10,12
D2S133817,19
D3S135815
D5S8189,10
D7S82011,12
D8S117910
D13S3178,13
D16S5399,10
D18S5114,21
D19S43314,15.2
D21S1129,30.3
FGA24
Penta D12,13
Penta E15,16
TH019
TPOX8
vWA14,16

Run an STR similarity search on this cell line
Publications

PubMed=2504489
Tamura K., Shimizu K., Yamada M., Okamoto Y., Matsui Y., Park K.C., Mabuchi E., Moriuchi S., Mogami H.
Expression of major histocompatibility complex on human medulloblastoma cells with neuronal differentiation.
Cancer Res. 49:5380-5384(1989)

PubMed=2818910
Yamada M., Shimizu K., Tamura K., Okamoto Y., Matsui Y., Moriuchi S., Park K.C., Mabuchi E., Yamamoto K., Hayakawa T., Mogami H.
Establishment and biological characterization of human medulloblastoma cell lines.
No To Shinkei 41:695-702(1989)

CLPUB00094
Shimizu K., Yamada M., Moriuchi S., Tamura K., Okamoto Y., Park K.C., Mabuchi E., Miyao Y., Hayakawa T.
Human medulloblastoma cell lines: ONS-76 and ONS-81.
Hum. Cell 3:265-266(1990)

DOI=10.1007/978-4-431-68150-2_63
Shimizu K., Yamada M., Moriuchi S., Tamura K., Mabuchi E., Okamoto Y., Miyao Y., Park K.C., Hayakawa T.
Biological characterization of human medulloblastoma (ONS-76 and ONS-81) cell lines.
(In book chapter) Biological aspects of brain tumors. Proceedings of the 8th Nikko brain tumor conference, Karatsu (Saga) 1990; Tabuchi K. (eds.); pp.442-446; Springer; Tokyo; Japan (1991)

PubMed=1992917
Mabuchi E., Shimizu K., Yamada M., Moriuchi S., Park K., Tamura K., Hayakawa T., Mogami H.
In vitro chemosensitivity test with several antitumor agents against eight malignant brain tumor cell-lines.
Gan To Kagaku Ryoho 18:233-238(1991)

DOI=10.1016/B978-0-12-333530-2.50005-8
Nister M., Westermark B.
Human glioma cell lines.
(In book chapter) Atlas of human tumor cell lines; Hay R.J., Park J.-G., Gazdar A.F. (eds.); pp.17-42; Academic Press; New York; USA (1994)

PubMed=20164919; DOI=10.1038/nature08768; PMCID=PMC3145113
Bignell G.R., Greenman C.D., Davies H.R., Butler A.P., Edkins S., Andrews J.M., Buck G., Chen L., Beare D., Latimer C., Widaa S., Hinton J., Fahey C., Fu B.-Y., Swamy S., Dalgliesh G.L., Teh B.T., Deloukas P., Yang F.-T., Campbell P.J., Futreal P.A., Stratton M.R.
Signatures of mutation and selection in the cancer genome.
Nature 463:893-898(2010)

PubMed=22460905; DOI=10.1038/nature11003; PMCID=PMC3320027
Barretina J.G., Caponigro G., Stransky N., Venkatesan K., Margolin A.A., Kim S., Wilson C.J., Lehar J., Kryukov G.V., Sonkin D., Reddy A., Liu M., Murray L., Berger M.F., Monahan J.E., Morais P., Meltzer J., Korejwa A., Jane-Valbuena J., Mapa F.A., Thibault J., Bric-Furlong E., Raman P., Shipway A., Engels I.H., Cheng J., Yu G.-Y.K., Yu J.-J., Aspesi P. Jr., de Silva M., Jagtap K., Jones M.D., Wang L., Hatton C., Palescandolo E., Gupta S., Mahan S., Sougnez C., Onofrio R.C., Liefeld T., MacConaill L.E., Winckler W., Reich M., Li N.-X., Mesirov J.P., Gabriel S.B., Getz G., Ardlie K., Chan V., Myer V.E., Weber B.L., Porter J., Warmuth M., Finan P., Harris J.L., Meyerson M.L., Golub T.R., Morrissey M.P., Sellers W.R., Schlegel R., Garraway L.A.
The Cancer Cell Line Encyclopedia enables predictive modelling of anticancer drug sensitivity.
Nature 483:603-607(2012)

PubMed=22951319; DOI=10.1093/jrr/rrs078; PMCID=PMC3534279
Sun L., Moritake T., Zheng Y.-W., Suzuki K., Gerelchuluun A., Hong Z.-S., Zenkoh J., Taniguchi H., Tsuboi K.
In vitro stemness characterization of radio-resistant clones isolated from a medulloblastoma cell line ONS-76.
J. Radiat. Res. 54:61-69(2013)

PubMed=25877200; DOI=10.1038/nature14397
Yu M., Selvaraj S.K., Liang-Chu M.M.Y., Aghajani S., Busse M., Yuan J., Lee G., Peale F.V., Klijn C., Bourgon R., Kaminker J.S., Neve R.M.
A resource for cell line authentication, annotation and quality control.
Nature 520:307-311(2015)

PubMed=26589293; DOI=10.1186/s13073-015-0240-5; PMCID=PMC4653878
Scholtalbers J., Boegel S., Bukur T., Byl M., Goerges S., Sorn P., Loewer M., Sahin U., Castle J.C.
TCLP: an online cancer cell line catalogue integrating HLA type, predicted neo-epitopes, virus and gene expression.
Genome Med. 7:118.1-118.7(2015)

PubMed=27397505; DOI=10.1016/j.cell.2016.06.017; PMCID=PMC4967469
Iorio F., Knijnenburg T.A., Vis D.J., Bignell G.R., Menden M.P., Schubert M., Aben N., Goncalves E., Barthorpe S., Lightfoot H., Cokelaer T., Greninger P., van Dyk E., Chang H., de Silva H., Heyn H., Deng X.-M., Egan R.K., Liu Q.-S., Mironenko T., Mitropoulos X., Richardson L., Wang J.-H., Zhang T.-H., Moran S., Sayols S., Soleimani M., Tamborero D., Lopez-Bigas N., Ross-Macdonald P., Esteller M., Gray N.S., Haber D.A., Stratton M.R., Benes C.H., Wessels L.F.A., Saez-Rodriguez J., McDermott U., Garnett M.J.
A landscape of pharmacogenomic interactions in cancer.
Cell 166:740-754(2016)

PubMed=27498314; DOI=10.1016/j.jbiotec.2016.07.028
Ivanov D.P., Coyle B., Walker D.A., Grabowska A.M.
In vitro models of medulloblastoma: choosing the right tool for the job.
J. Biotechnol. 236:10-25(2016)

PubMed=27812533; DOI=10.1016/j.dib.2016.10.004; PMCID=PMC5079239
Ivanov D.P., Walker D.A., Coyle B., Grabowska A.M.
Data on the number and frequency of scientific literature citations for established medulloblastoma cell lines.
Data Brief 9:696-698(2016)

PubMed=30894373; DOI=10.1158/0008-5472.CAN-18-2747; PMCID=PMC6445675
Dutil J., Chen Z.-H., Monteiro A.N.A., Teer J.K., Eschrich S.A.
An interactive resource to probe genetic diversity and estimated ancestry in cancer cell lines.
Cancer Res. 79:1263-1273(2019)

PubMed=31068700; DOI=10.1038/s41586-019-1186-3; PMCID=PMC6697103
Ghandi M., Huang F.W., Jane-Valbuena J., Kryukov G.V., Lo C.C., McDonald E.R. 3rd, Barretina J.G., Gelfand E.T., Bielski C.M., Li H.-X., Hu K., Andreev-Drakhlin A.Y., Kim J., Hess J.M., Haas B.J., Aguet F., Weir B.A., Rothberg M.V., Paolella B.R., Lawrence M.S., Akbani R., Lu Y.-L., Tiv H.L., Gokhale P.C., de Weck A., Mansour A.A., Oh C., Shih J., Hadi K., Rosen Y., Bistline J., Venkatesan K., Reddy A., Sonkin D., Liu M., Lehar J., Korn J.M., Porter D.A., Jones M.D., Golji J., Caponigro G., Taylor J.E., Dunning C.M., Creech A.L., Warren A.C., McFarland J.M., Zamanighomi M., Kauffmann A., Stransky N., Imielinski M., Maruvka Y.E., Cherniack A.D., Tsherniak A., Vazquez F., Jaffe J.D., Lane A.A., Weinstock D.M., Johannessen C.M., Morrissey M.P., Stegmeier F., Schlegel R., Hahn W.C., Getz G., Mills G.B., Boehm J.S., Golub T.R., Garraway L.A., Sellers W.R.
Next-generation characterization of the Cancer Cell Line Encyclopedia.
Nature 569:503-508(2019)

PubMed=31346954; DOI=10.1007/s10616-019-00332-3; PMCID=PMC6787134
Bonfim Silva R., Salomao K.B., de Andrade Pimentel T.V.C., de Oliveira Menezes C.C.B., Palma P.V.B., Fontes A.M.
Biological characterization of the UW402, UW473, ONS-76 and DAOY pediatric medulloblastoma cell lines.
Cytotechnology 71:893-903(2019)

PubMed=35839778; DOI=10.1016/j.ccell.2022.06.010; PMCID=PMC9387775
Goncalves E., Poulos R.C., Cai Z.-X., Barthorpe S., Manda S.S., Lucas N., Beck A., Bucio-Noble D., Dausmann M., Hall C., Hecker M., Koh J., Lightfoot H., Mahboob S., Mali I., Morris J., Richardson L., Seneviratne A.J., Shepherd R., Sykes E., Thomas F., Valentini S., Williams S.G., Wu Y.-X., Xavier D., MacKenzie K.L., Hains P.G., Tully B., Robinson P.J., Zhong Q., Garnett M.J., Reddel R.R.
Pan-cancer proteomic map of 949 human cell lines.
Cancer Cell 40:835-849.e8(2022)

Cross-references
Cell line collections (Providers) BCRJ; 0294
JCRB; IFO50355
Cell line databases/resources CLO; CLO_0009900
cancercelllines; CVCL_1624
Cell_Model_Passport; SIDM00567
Cosmic-CLP; 909248
DepMap; ACH-000776
LINCS_LDP; LCL-1578
Biological sample resources BioSample; SAMN03470886
BioSample; SAMN10988186
CRISP screens repositories BioGRID_ORCS_Cell_line; 513
Chemistry resources ChEMBL-Cells; CHEMBL3308540
ChEMBL-Targets; CHEMBL2366068
GDSC; 909248
PharmacoDB; ONS76_1201_2019
PubChem_Cell_line; CVCL_1624
Encyclopedic resources Wikidata; Q54936426
Experimental variables resources EFO; EFO_0022571
Gene expression databases ArrayExpress; E-MTAB-783
ArrayExpress; E-MTAB-2770
ArrayExpress; E-MTAB-3610
GEO; GSM887477
GEO; GSM888558
GEO; GSM919364
GEO; GSM1670306
Polymorphism and mutation databases Cosmic; 909248
Cosmic; 1995603
IARC_TP53; 21602
LiGeA; CCLE_428
Progenetix; CVCL_1624
Proteomic databases PRIDE; PXD030304
Sequence databases EGA; EGAS00001000978
Entry history
Entry creation04-Apr-2012
Last entry update10-Sep-2024
Version number43